The Effect of Rosmarinic Acid Against Ovarian and Lung Injuries Induced by Ovarian Torsion Detorsion in Rats

Year 2023, , 47 - 52, 31.07.2023

Ayhan Tanyeli , Fazile Nur Ekinci Akdemir , Derya Güzel , Kardelen Erdoğan , Ersen Eraslan , Gökhan Bilgin , Mustafa Can Güler

https://doi.org/10.21765/pprjournal.1304994

Abstract

Background: Here, we purposed to find out the effects of two different Rosmarinic acid (RA) doses against lung injury caused by ovarian ischemia-reperfusion. Materials and Methods: We planned the groups as sham, ovarian torsion detorsion (O/TD; 3hours torsion/3hours detorsion), RA 40 mg/kg (40 mg/kg RA+O/TD), and RA 80 mg/kg (80 mg/kg RA+O/TD) groups. Following the experimental procedure, we sacrificed the rats and then, collected the lung and ovarian tissues for biochemical evaluations. Result: Total oxidant status (TOS), myeloperoxidase (MPO) activity, malondialdehyde (MDA) levels, and oxidative stress index (OSI) were elevated in the O/TD group compared to the sham group. These parameters declined due to low and high doses of RA administration. Total antioxidant status (TAS) level and superoxide dismutase (SOD) activity diminished in the O/TD group while increasing in RA treatment groups. However, the high dose of RA treatment group enhanced the antioxidant activity further and reduced the oxidant parameters compared to the low dose RA treatment group. Conclusion: In this study, RA treatment reduced O/TD-induced ovarian and lung injuries in the experimental animals.

Keywords

Rosmarinic acid, torsion detorsion, ovary, rat

Over Torsiyon Detorsiyon Kaynaklı Over ve Akciğer Hasarına Karşı Rosmarinik Asidin Etkisi

Abstract

Amaç: Bu çalışmada over torsiyon detorsiyonunun neden olduğu akciğer hasarına karşı iki farklı Rosmarinic asit (RA) dozunun etkilerini bulmayı amaçladık. Gereç ve Yöntem: Grupları sham, over torsiyon detorsiyon (O/TD; 3 saat torsiyon/3 saat detorsiyon), RA 40 mg/kg (40 mg/kg RA+O/TD) ve RA 80 mg/kg (80 mg/kg RA+O/TD) olarak planladık. Deneyin ardından sıçanları sakrifiye edip biyokimyasal değerlendirmeler için akciğer ve over dokularını aldık. Bulgular: Total oksidan statüsü (TOS), myeloperoksidaz (MPO) aktivitesi, malondialdehit (MDA) seviyeleri ve oksidatif stres indeksi (OSI), O/TD grubunda sham gruba kıyasla yükselmişti. Bu parametreler, düşük ve yüksek doz RA uygulaması sonucunda azalma gösterdi. Total antioksidan statüsü (TAS) düzeyi ve süperoksit dismutaz (SOD) aktivitesi, RA tedavi gruplarında artarken O/TD grubunda azaldı. Ancak, yüksek doz RA tedavi grubu, düşük doz RA tedavi grubuna kıyasla antioksidan aktiviteyi daha da arttırıp oksidan parametreleri azaltmıştır. Sonuç: Mevcut çalışmada RA tedavisi sonucu deney hayvanlarında O/TD'nin neden olduğu over ve akciğer hasarı azalmıştır.

Keywords

Rosmarinik asit, torsiyon detorsiyon, over, rat

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