Year 2020, Volume 8 , Issue 1, Pages 1 - 6 2020-04-30

Detection of Human Norovirus among Children with Gastroenteritis in Diyala Governorate

Marwa Ghazzay DİRAA [1] , Jalil İbrahim AL-EZZİ [2] , Areej Atiya HUSSEİN [3]


Background: Acute gastroenteritis remains a global public health problem and human noroviruses persist a cause of gastroenteritis. Objectives: To determine the rate of human norovirus infections among children with gastroenteritis in Diyala governorate by enzyme linked immunosorbent assay and immuneochromatography, also to assess genogroup 1(GGI) and genogroup 2(GGII) by nested polymerase chain reaction among study population. Patients and methods: Cross sectional study were carried out on 182 children under five years old with acute gastroenteritis who attended the emergency department of pediatrics in Al-Batool Teaching Hospital in Baqubah city, during the period from 6th September 2018 till 4th of March 2019. Stool samples were collected from each participant and stored as frozen at -70 °C to use for enzyme linked immunosorbent assay and quick qualitative immunochromatographic test, finally positive results for human norovirus was used for RNA extraction and nested polymerase chain reaction. Results: The rate of human norovirus infection was 6.04%, most common among females 6 (54.55%). Age group (1-12) months showed highest frequency 8 (72.73%). The primary education of the mothers was noted in (54.55 %) followed by (18.18 %) for each secondary and higher education. Children with artificial milk feeding constituted (81.82%) and (18.18%) were mixed feeding, while no positive results recorded among children with breast feeding. The highest infection rate was noticed among patients used filtered and boiled water (54.54 %) followed by filtered water (27.28 %). Among clinical features caused by virus infection, abdominal pain constituted 11(100%), vomiting 10(90.09%), and nausea was noted in 7(63.63%). The results of nested polymerase chain reaction demonstrated that only one case was positive for human norovirus genogroup 2(GGII). Conclusion: Human norovirus genogroup II appear to play a major role in acute gastroenteritis among children.
Gastroenteritis, human norovirus, PCR
  • 1. Plants-Paris, K., Bishoff, D., Oyaro, M.O., Mwinyi, B., Chappell, C., Kituyi, A., Nyangao, J., Mbatha, D. and Darkoh, C. (2019). Prevalence of Clostridium difficile infections among kenyan children with diarrhea. Pubmed-NCBI; (19)30035-9. 2. Dennehy, P.H. (2005). Acute diarrheal disease in children: epidemiology, prevention, and treatment. Infect. Dis. Clin. North. Am., 19(3): 585-602. 3. Kabayiza, J.C. (2014). Acute gastroenteritis in Rwandan children under five years of age investigated by real-time PCR, M.Sc. Thesis, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg. 4. Trang, N.V., Tamura, T. and Garcia, C. (2012). Detection and molecular characterization of noroviruses and sapoviruses in children admitted to hospital with acute gastroenteritis in Vietnam, J. Med. Virol., 84: (2) 290-297. 5. Krisztián, B., Mary, K., Vito, M. and Umesh, D. (2018). Viral gastroenteritis, Lancet, 392(10142):175-186. 6. Green, K.Y. (2013). Caliciviridae: the noroviruses. In Knipe, D.M., Howley, P.M., Cohen, J.I., Griffin, D.E., Lamb, R.A., Martin, M.A., Racaniello, V.R. and Roizman, B. (ed), Fields Virology, 6th Ed., Lippincott Williams and Wilkins, Philadelphia, PA; 1: 582-608. 7. Vinjé, J. (2015). Advances in laboratory methods for detection and typing of Human norovirus, J. Clin. Microbiol., 53:373-381. 8. Lias, C., Paul, D., Michael, L., Boyd, Y., Matthew, H., Kari D., Rachel, L., and Ralph ,S. (2019). Human norovirus epitope D plasticity allows escape from antibody immunity without loss of capacity for binding cellular Ligands, J. Virol., 15; 93(2): e01813-18. 9. Centers for Disease Control and Prevention. (2011). Updated norovirus outbreak management and disease prevention guidelines. Morbidity and mortality weekend report. 60(3):1-15. https://www.cdc.gov/mmwr/pdf/rr/rr6003.pdf 10. Sattar, B. and Shashank, S. (2018). Bacterial gastroenteritis. Stat Pearls Publishing - Bookshelf - NCBI, available https://www.ncbi.nlm.nih.gov/books/NBK513295/ 11. Bartsch, S.M., Lopman, B.A., Ozawa, S., Hall, A.J. and Lee, B.Y. (2016). Global economic burden of norovirus gastroenteritis, PLoS ONE, 26;11(4): e0151219. 12. Kitajima, M., Oka, T., Haramoto, E., Phanuwan, C., Takeda, N. and Katayama, K. (2012). Genetic diversity of genogroup IV noroviruses in wastewater in Japan. Letters in Applied Microbiology., 2011;52:181–184. 13. Al-Ali, R.M., Chehadeh, W., Hamze, M., Dabboussi, F., Hlais, S. and Mallat, H. (2011). First description of gastroenteritis viruses in Lebanese children: a pilot study, J. Infect. Public. Health., 4(2):59-64. 14. Thwiny, H.T., Hassan, J.H. (2015). Detection of norovirus in stool samples by RT-PCR from under five years age children hospitalized for acute gastroenteritis in Basrah, Iraq, Journal of Thi-Qar Science, 5(2): 47-53. 15. Ben Salem-Ben Nejma, I., Hassine, Z.M., Hassine, F., Sdiri-Loulizi, K., Ben Said, M. and Aouni, M. (2014). Etiology of acute diarrhea in Tunisian children with emphasis on diarrheagenic Escherichia coli: Prevalence and identification of E. Coli virulence markers. Iran, J. Public Health., 43(7):947-960. 16. Tayeb, H.T., Dela-Cruz, D.M., Al-Qahtani, A., Al-Ahdal, M.N. and Carter, M.J. (2008). Enteric viruses in pediatric diarrhea in Saudi Arabia, J. Med. Virol., 80 (11):1919-1929. 17. Al-Moussawi, D.K., Rahman, L.C. and Basim, A.J. (2018). Genotyping and phylogenic of norovirus as main cause of children gastritis in Thi Qar province, University of Thi - Qar Journal of Science, 6(4) :35-41. 18. Mohammed, N.S., Mohammed, F.H. and Haider, K.A. (2016). Detection of norovirus and rotavirus in children under five years during winter 2012-2013 in Mosul province, I.A.S.J., 19(3): 101-107. 19. Al-Marsome, H.T., Jabbar, S.H., Hala, S.A., and Abdulrhman M.H.H. (2016). Diagnosis of noroviruses in Iraqi children suffering from gastroenteritis by enzyme linked assay and conventional PCR, I.A.S.J., 34(1): 42-49. 20. Al-Mashhadani, M.N., Nakagomi, O., Dove, W., Ahmed, H., Nakagomi T., Hart C.A. and Cunliffe N.A. (2008). Norovirus gastroenteritis among children in Iraqi Kurdistan, J. Med. Virol., 80 (3): 506-9. 21. Al-Khoweledy, A.J.S. (2015). Investigation of norovirus among diarrheal children in Al-Najaf province. IASJ., 8(3): 1-6. 22. Lorrot, M., Bon, F., El Hajje, M.J., Aho, S., Wolfer, M. and Giraudon, H. (2011). Epidemiology and clinical features of gastroenteritis in hospitalised children: prospective survey during a 2-year period in a Parisian hospital, France, Eur. J. Clin. Microbiol. Infect. Dis., 30 (3):361-368. 23. Huynen, P., Mauroy, A., Martin, C., Savadogo, L.GB., Boreux, R., Thiry, E. and De Mol, P. (2019). Molecular epidemiology of norovirus infections in symptomatic and asymptomatic children from Bobo Dioulasso, Burkina Faso, J. Clin. Virol., 58(3), 515-521. 24. Qi, L.M., Xinzhi, X., Ying, X., Hua, L., Huangcheng, S., Wenwen, D., Wenge, T., Tao, S., and Qin, L. (2017). Outbreak of acute gastroenteritis caused by norovirus genogroup II attributed to contaminated cold dishes on a cruise ship in Chongqing, China. Int. J. Environ. Res. Public Health., 15: 2823. 25. Hussein, A.A., Rawaa, A.H. and Mohammed, J.S. (2018). Enteric Viruses co-infection with giardiasis among diarrheal children in Diyala province – Iraq, J. Pure Appl. Microbiol., 12(2): 793-799. 26. Mahmood, D.B., Raghad, A., Faisal, G.A. and Rafah, A.S. (2015). Detection of Adenovirus antigen and its correlation with clinical signs among children with gastroenteritis, IJS, 56(2): 1633-1638. 27. Webb, A. and Starr, M. (2005). Acute gastroenteritis in children. Australian family physician, 34(4): 227-231. 28. Castilho,J.R., Veridiana, M., Hugo, R.R., Ulysses, F., Jan, V. and Maria, L. (2006). Genetic diversity of norovirus among children with gastroenteritis in São Paulo state, Brazil. JCM (Journal of clinical microbiology). 29. Al-Yassin, S.T. (1999). A study of cryptosporidiosis in immunocompromised patients, M.Sc. Thesis, College of Medicine, Tikrit University. 30. Debasism, B. and Ahemed, T. (2013). Study of knowledge, attitude and practice among mothers towards acute respiratory infection in urban and rural communitiesof burdwan district, West Bengal, India. Rev Progr., 1(8):1-6. 31. Ujunwa, F. (2014). Ezeonu C. risk factors for acute respiratory tract infections in under-five children in Enugu Southeast Nigeria. Ann Med Health Sci Res., 4(1):95–9. 32. Makita, K., Hayakawa, Y., Okame, M., Homma, K., Phan, T.G., Okitsu, S., and Ushijima, H. (2006). First detection of IgA against norovirus in breast milk, Clin. lab., 53(3-4): 125-128. 33. Al-Ameedi, R.A and Al-Amar, M.H. (2015). Detection of norovirus and adenovirus type 40/41 in diarrhea among children aged 1-60 months, in Al-Najaf province, Iraq, I.J.A.R., 3(11), 1391-1398. 34. Eftim, S.E., Hong, T., Soller, J., Boehm, A., Warren, I., Ichida, A., and Nappier, S.P. (2017). Occurrence of norovirus in raw sewage - A systematic literature review and meta-analysis, Water Res., 111:366-374. 35. Lopman, B., Paul, G., Geun, W., Aron, J., Umesh, D. and Jan, V. (2012). Environmental transmission of human norovirus gastroenteritis, Science Direct, 2(1): 96-102. 36. Wang, Y., Lipengm H., Lifeng, P., Caoyi, X., Qing, L., Xuetao, Z., and Weiping, Z. (2018). Age, primary symptoms, and genotype characteristics of norovirus outbreaks in Shanghai schools in 2017, Sci. Rep., 8: 15238. 37. Timurkan, M., Aydin, H. and Aktaş, O. (2017). Frequency and molecular characterization of human norovirus in Erzurum- Turkey, Turk. J. Med. Sci., 47(3):960-966. 38. Mathew, J.L., Patwari, A.K., Gupta, P., Shah, D., Gera, T. and Gogia, S. (2019). Acute respiratory infection and pneumonia in India: a systematic review of literature for advocacy and action: UNICEF-PHFI series on newborn and child health, India. Indian Pediatr.,48(3):191–218. 39. Nirwati, H., Donato, C.M, Mawarti, Y., Mulyani, N.S., Ikram, A., Aman, A.T., Peppelenbosch, M.P., Soenarto, Y., Pan, Q. and Hakim, M.S. (2019). Norovirus and rotavirus infections in children less than five years of age hospitalized with acute gastroenteritis in Indonesia, Arch. Virol., 164(6):1515-1525. 40. Lauring, A.S., Frydman, J. and Andino, R. (2013). The role of mutation load robustness in RNA virus evolution. Nature Reviews, Microbiology, 11(5), 327-336.
Primary Language en
Subjects Health Care Sciences and Services
Journal Section Original Articles
Authors

Author: Marwa Ghazzay DİRAA
Institution: College of Medicine , University of Diyala
Country: Iraq


Orcid: 0000-0002-7541-4852
Author: Jalil İbrahim AL-EZZİ (Primary Author)
Institution: University of Diyala /College of Medicine
Country: Iraq


Orcid: 0000-0002-4284-9104
Author: Areej Atiya HUSSEİN
Institution: College of Medicine, University of Diyala
Country: Iraq


Supporting Institution none
Project Number 174
Dates

Publication Date : April 30, 2020

Bibtex @research article { pprjournal699412, journal = {Pediatric Practice and Research}, issn = {2147-6470}, address = {}, publisher = {MediHealth Academy Yayıncılık}, year = {2020}, volume = {8}, pages = {1 - 6}, doi = {}, title = {Detection of Human Norovirus among Children with Gastroenteritis in Diyala Governorate}, key = {cite}, author = {Al-ezzi̇, Jalil İbrahim and Hussei̇n, Areej} }
APA Di̇raa, M , Al-ezzi̇, J , Hussei̇n, A . (2020). Detection of Human Norovirus among Children with Gastroenteritis in Diyala Governorate . Pediatric Practice and Research , 8 (1) , 1-6 . Retrieved from https://dergipark.org.tr/en/pub/pprjournal/issue/56707/699412
MLA Di̇raa, M , Al-ezzi̇, J , Hussei̇n, A . "Detection of Human Norovirus among Children with Gastroenteritis in Diyala Governorate" . Pediatric Practice and Research 8 (2020 ): 1-6 <https://dergipark.org.tr/en/pub/pprjournal/issue/56707/699412>
Chicago Di̇raa, M , Al-ezzi̇, J , Hussei̇n, A . "Detection of Human Norovirus among Children with Gastroenteritis in Diyala Governorate". Pediatric Practice and Research 8 (2020 ): 1-6
RIS TY - JOUR T1 - Detection of Human Norovirus among Children with Gastroenteritis in Diyala Governorate AU - Marwa Di̇raa , Jalil İbrahim Al-ezzi̇ , Areej Hussei̇n Y1 - 2020 PY - 2020 N1 - DO - T2 - Pediatric Practice and Research JF - Journal JO - JOR SP - 1 EP - 6 VL - 8 IS - 1 SN - 2147-6470- M3 - UR - Y2 - 2020 ER -
EndNote %0 Pediatric Practice and Research Detection of Human Norovirus among Children with Gastroenteritis in Diyala Governorate %A Marwa Di̇raa , Jalil İbrahim Al-ezzi̇ , Areej Hussei̇n %T Detection of Human Norovirus among Children with Gastroenteritis in Diyala Governorate %D 2020 %J Pediatric Practice and Research %P 2147-6470- %V 8 %N 1 %R %U
ISNAD Di̇raa, Marwa , Al-ezzi̇, Jalil İbrahim , Hussei̇n, Areej . "Detection of Human Norovirus among Children with Gastroenteritis in Diyala Governorate". Pediatric Practice and Research 8 / 1 (April 2020): 1-6 .
AMA Di̇raa M , Al-ezzi̇ J , Hussei̇n A . Detection of Human Norovirus among Children with Gastroenteritis in Diyala Governorate. pediatr pract res. 2020; 8(1): 1-6.
Vancouver Di̇raa M , Al-ezzi̇ J , Hussei̇n A . Detection of Human Norovirus among Children with Gastroenteritis in Diyala Governorate. Pediatric Practice and Research. 2020; 8(1): 1-6.