Düşük Doz Doksisiklin ve Kişniş ile Tedavi Edilen Deneysel Periodontitisde Serum Antioksidan Seviyelerinin ve İnflamatuar Hücre Yoğunluğunun Karşılaştırılması

Year 2022, Volume: 12 Issue: 3, 445 - 452, 20.09.2022

Şeyma Bozkurt Doğan Umut Ballı Figen Öngöz Dede Kanat Gülle Bahattin Avcı Hatice Çölgeçen Meryem Akpolat Ferah Mazlum Bülent Kurtiş

https://doi.org/10.33631/sabd.1054420

Abstract

Amaç: Bu çalışmanın amacı deneysel olarak periodontitis oluşturulmuş ratlarda sistemik olarak uygulanmış düşük doz doksisiklinin (LDD) ve kişniş bitkisinin (CSL) serumdaki antioksidan enzim seviyeleri ve inflamatuar hücre miktarı üzerine olan etkilerini değerlendirmektir.
Gereç ve Yöntemler: Çalışmanın başlangıncında 40 adet Wistar Albino erkek rat rastgele beş eşit gruba ayrıldı. Grup 1: periodontal sağlıklı; grup 2: periodontitis; grup 3: periodontitis + CSL (32 mg/kg); grup 4: periodontitis +CSL (200mg/kg); grup 5: periodontitis + doksisiklin hidroklorid (6 mg/kg). Elde edilen serum örneklerinde superoksit dismutanz (SOD), glutatyon peroksidaz (GSH-Px), katalaz (CAT) seviyeleri ELISA metodu uygulanarak analiz edildi. İnflamatuar hücre yoğunluğu histopatolojik olarak değerlendirildi.
Bulgular: Serumdaki SOD seviyesi diğer gruplar ile karşılaştırıldığında istatistiksel olarak en düşük grup 1 ve istatistiksel olarak en yüksek ise grup 2 de bulundu. SOD seviyesi grup 1 ve 2 nin diğer gruplarla olan ikili karşılaştırmalarında istatistiksel olarak önemli bulurken, grup 3, 4 ve 5 arasında istatistiksel olarak önemli bir fark göstermedi. CAT seviyesi diğer gruplar ile karşılaştırıldığında istatistiksel olarak en düşük grup 2'de bulunurken, grup 1, 3, 4 ve 5 arasında önemli bir farklılık göstermedi. GSH-Px seviyesi gruplar arasında istatistiksel olarak önemli farklılık göstermedi. İnflamatuar hücre yoğunluğu grup 2 ve 4 de grup 1’e göre istatistiksel olarak önemli derecede yüksek bulundu. Grup 1, 3 ve 5 arasında inflamatuar hücre yoğunluğu açısından istatistiksel olarak önemli farklılık rapor edilmedi.
Sonuç: Serum SOD, CAT ve inflamatuar hücre yoğunluğu bakımından CSL ve LDD uygulama grupları arasında farklılık gözlenmedi. Bu yüzden, periodontitis tedavisinde farklı CSL dozajlarının uygulandığı çalışmaların yapılması gerektiğini düşünmekteyiz.

Keywords

Periodontitis, kişniş, antioksidan, düşük doz doksisiklin, serum

Supporting Institution

TÜBİTAK

Project Number

11S889

Comparison Effects of Serum Antioxidants Levels and Intensity of Inflammatory Cells in Experimental Periodontitis Treated with Low-Dose Doxycycline and Coriandrum Sativum L

Abstract

Aim: The aim of this study was to evaluate the effects of systemically-administered Coriandrum sativum L (CSL) and low dose doxycycline (LDD) on serum levels of antioxidant enzymes and intensity of inflammatory cells in rats with experimental periodontitis.
Material and Methods: Forty adult male Wistar Albino rats were divided randomly into 5 groups as follows: group 1: periodontally healthy (control); group 2: periodontitis; group 3: periodontitis+CSL (32mg/kg); group 4: periodontitis+CSL (200mg/kg); group 5: periodontitis+LDD (6 mg/kg). Serum gingival superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) levels were evaluated by ELISA. The intensity of inflammatory cells were evaluated by histopathologically.
Results: SOD levels were statistically lowest in group 1 and statistically were highest in group 2 than those of other groups. There was a statistical difference in SOD levels in paired comparisons of groups 1 and 2 with other groups. Its level did not have statistically significant among groups 3, 4 and 5. CAT levels were statistically lowest in group 2 than those of other groups, and no differences were reported among groups 1, 3, 4, and 5. GSH-Px levels did not have statistically significant among groups. Inflammatory cell infiltration was found to be statistically higher in groups 2 and 4 compared to group 1, and no statistical significance was reported among groups 1, 3, and 5.
Conclusion: CSL and LDD application groups did not show differences in terms of serum SOD, serum CAT, and intensity of inflammatory cells. Therefore, we suggest that the different dosages of CSL should be examined in the treatment of periodontitis.

Keywords

Periodontitis, Coriander Sativum L, Low Dose doxcycline, serum, antioxidant

Project Number

11S889

References

• Struch F, Dau M, Schwahn C, Biffar R, Kocher T, Meisel P. Interleukin-1 gene polymorphism, diabetes, and periodontitis: results from the Study of Health in Pomerania (SHIP). J Periodontol. 2008; 79: 501-7.
• Ramfjord SP, Knowles JW, Nissle RR, Shick RA, Burgett FG. Longitudinal study of periodontal therapy. J Periodontol. 1973; 44: 66-77.
• Aslani A, Ghannadi A, Najafi H. Design, formulation and evaluation of a mucoadhesive gel from Quercus brantii L. and coriandrum sativum L. as periodontal drug delivery. Adv Biomed Res. 2013; 2: 21.
• Baehni PC, Takeuchi Y. Anti-plaque agents in the prevention of biofilm-associated oral diseases. Oral Dis. 2003; 9 Suppl 1:23-9.
• Preshaw PM, Hefti AF, Jepsen S, Etienne D, Walker C, Bradshaw MH. Subantimicrobial dose doxycycline as adjunctive treatment for periodontitis. A review. J Clin Periodontol. 2004; 31: 697-707.
• Preshaw PM, Hefti AF, Novak MJ, Michalowicz BS, Pihlstrom BL, Schoor R. et al. Subantimicrobial dose doxycycline enhances the efficacy of scaling and root planing in chronic periodontitis: a multicenter trial. J Periodontol. 2004; 75: 1068-76.
• Golub LM, Ramamurthy NS, McNamara TF, Greenwald RA, Rifkin BR. Tetracyclines inhibit connective tissue breakdown: new therapeutic implications for an old family of drugs. Crit Rev Oral Biol Med. 1991; 2: 297-321.
• Chapple IL, Matthews JB. The role of reactive oxygen and antioxidant species in periodontal tissue destruction. Periodontol 2000. 2007; 43: 160-232.
• Chapple IL. Role of free radicals and antioxidants in the pathogenesis of the inflammatory periodontal diseases. Clin Mol Pathol. 1996; 49:M247-55.
• Yagan A, Kesim S, Liman N. Effect of low-dose doxycycline on serum oxidative status, gingival antioxidant levels, and alveolar bone loss in experimental periodontitis in rats. J Periodontol. 2014; 85: 478-89.
• Oktay S, Chukkapalli SS, Rivera-Kweh MF, Velsko IM, Holliday LS, Kesavalu L. Periodontitis in rats induces systemic oxidative stress that is controlled by bone-targeted antiresorptives. J Periodontol. 2015; 86: 137-45.
• Yaghini J, Shahabooei M, Aslani A, Zadeh MR, Kiani S, Naghsh N. Efficacy of a local-drug delivery gel containing extracts of Quercus brantii and Coriandrum sativum as an adjunct to scaling and root planing in moderate chronic periodontitis patients. J Res Pharm Pract. 2014; 3: 67-71.
• Tang EL, Rajarajeswaran J, Fung SY, Kanthimathi MS. Antioxidant activity of Coriandrum sativum and protection against DNA damage and cancer cell migration. BMC Complement Altern Med. 2013; 13: 347.
• Soares BV, Morais SM, dos Santos Fontenelle RO, Queiroz VA, Vila-Nova NS, Pereira CMC, et al. Antifungal activity, toxicity and chemical composition of the essential oil of Coriandrum sativum L. fruits. Molecules. 2012; 17: 8439-48.
• Chithra V, Leelamma S. Coriandrum sativum changes the levels of lipid peroxides and activity of antioxidant enzymes in experimental animals. Indian J Biochem Biophys. 1999; 36: 59-61.
• Nair V, Singh S, Gupta YK. Evaluation of disease modifying activity of Coriandrum sativum in experimental models. Indian J Med Res. 2012; 135: 240-5.
• Nair V, Singh S, Gupta YK. Anti-granuloma activity of Coriandrum sativum in experimental models. J Ayurveda Integr Med .2013; 4: 13-8.
• Sreelatha S, Inbavalli R. Antioxidant, antihyperglycemic, and antihyperlipidemic effects of Coriandrum sativum leaf and stem in alloxan-induced diabetic rats. J Food Sci. 2012; 77: T119-23.
• Ozdemir SP, Kurtis B, Tuter G, Bozkurt S, Gultekin SE, Senguven B. Effects of low-dose doxycycline and bisphosphonate clodronate on alveolar bone loss and gingival levels of matrix metalloproteinase-9 and interleukin-1beta in rats with diabetes: a histomorphometric and immunohistochemical study. J Periodontol. 2012; 83: 1172-82.
• Ramamurthy NS, Rifkin BR, Greenwald RA, Xu JW, Liu Y, Turner G, et al. Inhibition of matrix metalloproteinase-mediated periodontal bone loss in rats: a comparison of 6 chemically modified tetracyclines. J Periodontol. 2002; 73: 726-34.
• Koseoglu S, Hatipoglu M, Saglam M, Enhos S, Esen HH. Interleukin-33 could play an important role in the pathogenesis of periodontitis. J Periodontal Res. 2015; 50:525-34
• Aslani A, Rafiei S. Design, formulation and evaluation of nicotine chewing gum. Adv Biomed Res. 2012; 1: 57.
• Sachin G. Fulbel NNM, Nilima S. Rajhans, Monali B. Pimple, Neha V. Kumthekar, Gaurav S. Lodha, Vivek R. Devani. Efficacy of herbal mucoadhesive gel with extracts of Coriandrum sativum and curcumin as local drug delivery in the treatment of chronic periodontitis - A clinicomicrobiological trial. Saint Int Dent J. 2020; 4: 30-6.
• Listgarten MA. Similarity of epithelial relationships in the gingiva of rat and man. J Periodontol. 1975; 46: 677-80.
• Bezerra MM, de Lima V, Alencar VB, Vieira IB, Brito GA, Ribeiro RA, al. Selective cyclooxygenase-2 inhibition prevents alveolar bone loss in experimental periodontitis in rats. J Periodontol. 2000; 71: 1009-14.
• Bezerra MM, Brito GA, Ribeiro RA, Rocha FA. Low-dose doxycycline prevents inflammatory bone resorption in rats. Braz J Med Biol Res. 2002; 35: 613-6.
• Alencar VB, Bezerra MM, Lima V, Abreu AL, Brito GA, Rocha FAC, et al. Disodium chlodronate prevents bone resorption in experimental periodontitis in rats. J Periodontol. 2002; 73: 251-6.
• Ramamurthy N, Bain S, Liang CT, Barnes J, Llavaneras A, Liu Y, et al. A combination of subtherapeutic doses of chemically modified doxycycline (CMT-8) and a bisphosphonate (clodronate) inhibits bone loss in the ovariectomized rat: a dynamic histomorphometric and gene expression study. Curr Med Chem. 2001; 8: 295-303.
• Buduneli E, Vardar-Sengul S, Buduneli N, Atilla G, Wahlgren J, Sorsa T. Matrix metalloproteinases, tissue inhibitor of matrix metalloproteinase-1, and laminin-5 gamma2 chain immunolocalization in gingival tissue of endotoxin-induced periodontitis in rats: effects of low-dose doxycycline and alendronate. J Periodontol. 2007; 78: 127-34.
• Llavaneras A, Ramamurthy NS, Heikkila P, Teronen O, Salo T, Ryan ME, et al. A combination of a chemically modified doxycycline and a bisphosphonate synergistically inhibits endotoxin-induced periodontal breakdown in rats. J Periodontol. 2001; 72: 1069-77.
• Buduneli E, Vardar S, Buduneli N, Berdeli AH, Turkoglu O, Başkesen A, et al. Effects of combined systemic administration of low-dose doxycycline and alendronate on endotoxin-induced periodontitis in rats. J Periodontol. 2004; 75: 1516-23.
• Park G, Kim HG, Kim YO, Park SH, Kim SY, Oh MS. Coriandrum sativum L. protects human keratinocytes from oxidative stress by regulating oxidative defense systems. Skin pharmacology and physiology. 2012; 25: 93-9.
• Akalin FA, Toklu E, Renda N. Analysis of superoxide dismutase activity levels in gingiva and gingival crevicular fluid in patients with chronic periodontitis and periodontally healthy controls. J Clin Periodontol. 2005; 32: 238-43.
• Panjamurthy K, Manoharan S, Ramachandran CR. Lipid peroxidation and antioxidant status in patients with periodontitis. Cell Mol Biol Lett. 2005; 10: 255-64.
• Baltacioglu E, Akalin FA, Alver A, Balaban F, Unsal M, Karabulut E. Total antioxidant capacity and superoxide dismutase activity levels in serum and gingival crevicular fluid in post-menopausal women with chronic periodontitis. J Clin Periodontol. 2006; 33: 385-92.
• Canakci V, Yildirim A, Canakci CF, Eltas A, Cicek Y, Canakci H. Total antioxidant capacity and antioxidant enzymes in serum, saliva, and gingival crevicular fluid of preeclamptic women with and without periodontal disease. J Periodontol .2007; 78: 1602-11.
• Aziz AS, Kalekar MG, Suryakar AN, Benjamin T, Prakashan MJ, Ahmed BMN, et al. Assessment of some biochemical oxidative stress markers in male smokers with chronic periodontitis. Indian J Clin Biochem. 2013; 28: 374-80.
• Tsai CC, Chen HS, Chen SL, Ho YP, Ho KY, Wu YM, et al. Lipid peroxidation: a possible role in the induction and progression of chronic periodontitis. J Periodontal Res. 2005; 40: 378-84.
• Ellis SD, Tucci MA, Serio FG, Johnson RB. Factors for progression of periodontal diseases. J Oral Pathol Med. 1998; 27: 101-5.