The relationship between Thiol/disulfide homeostasis and endometrial hyperplasia in patients with abnormal uterine bleeding/

Abstract

Introduction: The role of oxidative stress and antioxidant capacity in the development of endometrial hyperplasia (EH) is controversial. Aim: The study aimed to evaluate Thiol/disulfide Homeostasis and ischemia modified albumin (IMA) levels in patients with EH without atypia. Materials and Methods: In this prospective case-control study, patients with EH without atypia (HP group) (n=28), patients with nonhyperplasia (proliferative/secretory/irregular proliferative/irregular secretory endometrium) (non-HP group) (n=28), and 28 healthy women (control group) were included. The patient's clinical characteristics, serum Thiol/disulfide parameters, and IMA levels were compared between groups. Results: A total of 84 patients were included in the study. Patients’ mean age, BMI, and mean native thiol (-SH-), total thiol (-SH-+-SS-), disulfide (-SS-), and IMA levels were similar among the three groups. The -SS- /-SH- ratio was higher in the HP group than the non-HP group. -SS- /-SH-+-SS- ratio was higher in the HP group vs. the other two groups. The -SS- /-SH-+-SS ratio was higher in the HP group vs. the non-HP group. -SH-/ -SH-+-SS- ratio was lower in the HP group than in the non-HP group. ET was greater in the HP group than in the non-HP and control groups. ET was also significantly greater in the non-HP group vs. in the control group. -SS-/-SH- ratio was found to be predictive with 64% sensitivity and 68% specificity for EH (area under curve = 0.672, p = 0.01). Conclusion and Suggestions: The dynamic thiol/disulfide balance shifted to the disulfide side in women with endometrial hyperplasia.

Keywords

• Thiol/disulfide homeostasis
• Oxidative stress
• Endometrial hyperplasia without atypia
• Ischemia modified albumin.

Anormal uterin kanamalı kadınlarda endometrial hiperplazi ve thiol disülfat homeostazis ilişkisi

Abstract

Giriş: Endometrial hiperplazi gelişimde oksidatif stres ve antioksidan kapasitenin rolü tartışmalıdır. Giriş: Endometrial hiperplazi gelişimde oksidatif stres ve antioksidan kapasitenin rolü tartışmalıdır. Amaç: Çalışma, Basit atipisiz endometrial hiperplazili hastalarda thiol-disulfat homeostazis ve iskemi-modifiye albumin (IMA) seviyelerini değerlendirmeyi amaçlamıştır. Gereç ve Yöntemler: Prospektif vaka kontrol dizaynlı çalışmada, atipisiz endometrial hiperplazili (HP grup) (n=28) hastalar, nonhiperplazi (proliferatif/sekretuar/düzensiz proliferatif/düzensiz sekretuar endometrium) saptanan hastalar (non-HP grup) (n=28) ve 28 sağlıklı kadın (kontrol grubu) çalışmaya dahil edildi. Hastaların klinik özellikleri, Thiol-disulfat parametreleri ve IMA seviyeleri gruplar arasında karşılaştırıldı. Bulgular: Çalışmaya toplam 84 hasta dahil edildi. Hastaların ortalama yaş, BMI, mean native thiol (-SH-), total thiol (-SH-+-SS-), disulfide (-SS-) ve IMA seviyeleri 3 grup arasında benzerdi. -SS-/-SH- ve -SS-/-SH-+-SS oranı HP grubunda non-HP grubundan anlamlı yüksekti. -SS-/-SH-+-SS- oranı HP grubunda diğer iki gruptan anlamlı yüksekti. -SH-/-SH-+-SS- oranı Hp grubunda non-HP grubuna göre anlamlı düşüktü. Endometrial kalınlık (ET), HP grubunda, non-HP ve kontrol grubuna göre daha kalındı. ET non-HP grubunda, kontrol grubundan anlamlı kalındı. -SS-/-SH- oranı %64 sensitivite, %68 spesifite ile endometrial hiperplaziyi öngörmektedir. Sonuç ve Öneriler: Endometrial hiperplazili kadınlarda Dinamik thiol-disülfat dengesi disülfat tarafına kaymıştır.

Keywords

• Thiol-Disülfat homeostazis
• Oksidatif stres
• Atipisiz endometrial hiperplazi
• İskemi-modifiye albumin

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