Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast

Year 2020, , 265 - 271, 01.02.2020

Pinar Buket Atalay Nur Kaluc Elif Ergin Cavusoglu

https://doi.org/10.16984/saufenbilder.559820

Cited By: 2

Abstract

Cancer
cells are defined by abnormal and unrestricted mitotic divisions, therefore
targeting mitosis is a useful strategy for cancer treatment. Two groups of
drugs that are most successfully used in the treatment of several types of
cancer, taxanes and vinca
alkaloids, exhibit their anti-tumor effects by causing a mitotic arrest.
However, not much is known about whether being arrested in mitosis affects the
sensitivity of cells to tumor-related stresses, such as oxidative and osmotic
stresses. In this study, we investigated whether mitosis affects the
sensitivity of Saccharomyces cerevisiae
cells to H₂O₂-induced oxidative stress and
sorbitol-induced osmotic stress. Mitotic and G1-arrests were induced by
nocodazole and alpha factor, respectively. The effects of nocodazole or alpha
factor treatments on the sensitivity of wild type (WT) and MAD3 deletion (mad3Δ) strains to
oxidative stress and osmotic stress were evaluated by the spotting and coloni
forming unit (cfu) assays as well as detection of reactive oxygen species (ROS)
production. Data were
analyzed using Student’s t-test and expressed as standard deviation (std), p<0.05 was considered significant. Our data indicate that mitosis
significantly increases resistance to oxidative stress_, however it
does not have any significant effect on the osmotic stress resistance in yeast.

Keywords

Oxidative stress, Osmotic stress, Mitosis, Saccharomyces cerevisiae

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