Isolation and Identification of Listeria spp. from White Cheese Samples Presented for Consumption in Istanbul

Year 2021, , 1253 - 1262, 31.12.2021

Nil Kaya , Elif Özlem Arslan Aydoğdu , Ayten Kimiran

https://doi.org/10.16984/saufenbilder.985810

Abstract

In this study, 119 feta cheese samples taken from different vendors in Istanbul were examined for the presence of L. monocytogenes. Isolates were confirmed by PCR method using iap and hlyA primers, and the antibiotic susceptibility of the identified strains was performed by the Kirby-Bauer protocol. Seven Listeria spp. was isolated from three (% 2.52) of 119 cheese samples analyzed. The seven Listeria spp. obtained from these three samples were found to contain the iap gene region but not the hlyA gene region. As a result of the sequence analysis using the 16S rRNA gene region, it was determined that these isolates were L. seeligeri. As a result of antibiotic susceptibility tests, it was observed that L. seeligeri isolates were showed ciprofloxacin (% 85.71) and penicillin (% 42.85) resistance. All strains were susceptible to amikacin, amoxicillin/clavulanic acid, chloramphenicol, rifampin, gentamicin, cefaclor, ampicillin, trimethoprim-sulfamethoxazole, tetracycline, vancomycin, and clarithromycin antibiotics. The detection of Listeria bacteria in feta cheese samples made with pasteurized milk revealed that packaging, distribution, and storage practices following the pasteurization process should be followed more strictly. It is recommended to apply controls at each stage to prevent contamination.

Keywords

L. monocytogenes, Feta cheese, iap, hlyA, Antibiotic sensitivity

Project Number

22416

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