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The metal effect on the Polyphenol oxidase enzyme from borage(Trachystemon orientalis) plant

Year 2013, Volume: 17 Issue: 3, 399 - 405, 01.06.2013

Abstract

In this study, Polyphenol oxidase was extracted from Borage plant (Trachystemon orientalis) and the crude extract was used for the assays. Its pH and temperature optima for 4-methyl cathecol were 5.0 and 5° C, respectively. Km of this enzyme was 4.55 mM for 4-methyl cathecol. We also found that the enzyme was activated by Fe3+ , Mg 2+ , Zn 2+ , Cu 2+ , Ca 2+ , K + , but inhibited by Hg2+ , Mn 2+ , Ni 2+ , Sn 2+ , Na + . Ba 2+ , Al 3+ and Pb2+ metal ions both activated and inhibited the enzyme at different concentrations.

References

  • (REFERENCES) Michalak A., Phenolic Compounds and Their Antioxidant Activity in Plants Growing under Heavy Metal Stress, Polish J. of Environ. Stud., 15 (4), 523-530, 2006.
  • Pe´rez-de-Moraa A., Burgosa P., Madejo´na E., Cabreraa F., Jaeckelb P., Schloterb M., Microbial Community Structure and Function in a Soil Contaminated by Heavy Metals: Effects of Plant Growth and Different Amendments, Soil Biology & Biochemistry, 38, 327-341, 2006.
  • Bhattacharya P. K., Metal Ions in Biochemistry, Alpha Science, London, 190-194, 2005.
  • Majer B. J., Tscherko D., Paschke A., Wennrich R., Kundi M., Kandeler E., Knasmüller S., Effects of Heavy Metal Contamination of Soils on Micronucleus Induction in Tradescantia and on Microbial Enzyme activities: a Comparative Investigation, Genetic Toxicology and Environmental Mutagenesis, 515, 111–124, 200 Dubovskiy I.M., Grizanova E.V., Ershova N.S., Rantala M.J., Glupov V.V., The Effects of Dietary Nickel on The Detoxification Enzymes, Innate Immunity and Resistance to The Fungus Beauveria bassiana in The Larvae of The Greater Wax Moth Galleria mellonella, Chemosphere, 85, 92-96, 2011.
  • Shen G., Lu Y., Zhou Q., Hong J., Interaction of Polycyclic Aromatic Hydrocarbons and Heavy Metals on Soil Enzyme, Chemosphere, 61, 1175–1182, 2005.
  • Xu H., Song P., Gu W., Yang Z., Effects of Heavy Metals on Production of Thiol Compounds and Antioxidant Enzymes in Agaricus bisporus, Ecotoxicology and Environmental Safety, 74, 1685-1692, 2011.
  • Kazımírova M., Slovák M., Effects of Heavy Metals and Fluorine on Phagocytosis and Phenoloxidase Activity in Mamestra brassicae, Eur. J. Entomol., 93, 467-473, 1996.
  • Gianfreda L., Ruggiero P., Soil Biology, Springer-Verlag Berlin Heidelberg, 257-311, 200 Nayeka S., Guptaa S., Sahab R.N., Metal Accumulation and Its Effects in Relation to Biochemical Response of Vegetables Irrigated with Metal Contaminated Water and Wastewater, Journal of Hazardous Materials, 178, 588–595, 20 Anjum N.A., Ahmad I., Mohmood I., Pacheco M., Duarte A.C., Pereira E., Umar S., Ahmad A., Khan N.A., Iqbal M., Prasad M.N.V., Modulation of Glutathione and Its Related Enzymes in Plants’ Responses to Toxic Metals and Metalloids, Environmental and Experimental Botany, 75, 307–324, 2012.
  • Saffar A., Najjar M.B.B., Mianabadi M., Activity of Antioxidant Enzymes in Response to Cadmium in Arabidopsis thaliana, Journal of Biological Sciences, 9 (1), 44-50, 2009.
  • Israr M., Jewella A., Kumarb D., Sahia S.V., Interactive Effects of Lead, Copper, Nickel and Zinc on Growth, Metal Uptake and Antioxidative Metabolism of Sesbania drummondii, Journal of Hazardous Materials, 186, 1520–1526, 2011.
  • Jaleel C.A., Jayakumar K., Chang-Xing Z., Azooz M.M., Effect of Soil Applied Cobalt on Activities of Antioxidant Enzymes in Arachis hypogaea, Global Journal of Molecular Sciences, 3 (2), 42-45, 2008.
  • Constabel C.P., Barbehenn R., Induced Plant Resistance to Herbivory, Springer, Dordrecht , 253-269, 2008.
  • Karagöz A., Cevahir G., Özcan T., Sadıkoğlu N., Yentür S., Kuru A., Bazı Yüksek Bitkilerden Hazırlanan Sulu Ekstrelerin Antiviral Aktivite Potansiyellerinin Değerlendirilmesi, Bitkisel İlaç Hammaddeleri Toplantısı-Bildiriler, Eskişehir, 318-321, 2002.
  • Akçin Ö.E., Kandemir N., Akçin Y., A Morphological and Anatomical Study on a Medicinal and Edible Plant Trachystemon orientalis (L.) G.Don (Boraginaceae) in the Black Sea Region, Turk J Bot, 28, 435-442, 200 Özen T., Antioxidant Activity of Wild Edible Plants in The Black Sea Region of Turkey, Grasas y Aceıtes, 61 (1), 86-94, 2010.
  • Ceyhun A.B., Şentürk M., Yerlikaya E., Erdoğan O., Küfrevioğlu Ö.İ., Ekinci D., Purification and Characterization of Carbonic Anhydrase from The Teleost Fish Dicentrarchus labrax (European seabass) Liver and Toxicological Effects of Metals on Enzyme Activity, Environmental Toxicology and Pharmacology, 32, 69-74, 2011.
  • Güngör K., Çağla Badem (Prunus dulcis) Bitkisinden Polifenol Oksidaz Enziminin Saflaştırılması ve Karakterizasyonu, Yüksek Lisans Tezi, Sakarya Üniversitesi Fen Bilimleri Enstitüsü, Sakarya, 2008.
  • Gao Z.J., Liu J.B., Xiao X.G., Purification and Characterisation of Polyphenol Oxidase from Leaves of Cleome gynandra L., Food Chemistry, 129, 1012-1018, 2011.
  • Yang C.P., Fujita S., Ashrafuzzaman M.D., Nakamura N., Hayashi N., Purification and Characterization of Polyphenol Oxidase from Banana (Musa sapientum L.) Pulp, J. Agric. Food Chem., 48, 2732-2735, 2000.
  • Aydemir T., Selected Kinetic Properties of Polyphenol Oxidase Extracted from Rosmarinus Officinalis L., International Journal of Food Properties, 13 (3), 475-485, 2010.
  • Gao Z.J., Han X.H., Xiao X.G., Purification and Characterisation of Polyphenol Oxidase from Red Swiss Chard (Beta vulgaris subspecies cicla) Leaves, Food Chemistry, 117, 342-348, 200

Kaldirik(Trachystemon orientalis) bitkisi Polifenol oksidaz enzimi üzerine metallerin etkisi

Year 2013, Volume: 17 Issue: 3, 399 - 405, 01.06.2013

Abstract

In this study, Polyphenol oxidase was extracted from Borage plant (Trachystemon orientalis) and the crude extract was used for the assays. Its pH and temperature optima for 4-methyl cathecol were 5.0 and 5°C, respectively. Km of this enzyme was 4.55 mM for 4-methyl cathecol. We also found that the enzyme was activated by Fe3+, Mg2+, Zn2+, Cu2+, Ca2+, K+, but inhibited by Hg2+, Mn2+, Ni2+, Sn2+, Na+. Ba2+, Al3+ and Pb2+ metal ions both activated and inhibited the enzyme at different concentrations.

References

  • (REFERENCES) Michalak A., Phenolic Compounds and Their Antioxidant Activity in Plants Growing under Heavy Metal Stress, Polish J. of Environ. Stud., 15 (4), 523-530, 2006.
  • Pe´rez-de-Moraa A., Burgosa P., Madejo´na E., Cabreraa F., Jaeckelb P., Schloterb M., Microbial Community Structure and Function in a Soil Contaminated by Heavy Metals: Effects of Plant Growth and Different Amendments, Soil Biology & Biochemistry, 38, 327-341, 2006.
  • Bhattacharya P. K., Metal Ions in Biochemistry, Alpha Science, London, 190-194, 2005.
  • Majer B. J., Tscherko D., Paschke A., Wennrich R., Kundi M., Kandeler E., Knasmüller S., Effects of Heavy Metal Contamination of Soils on Micronucleus Induction in Tradescantia and on Microbial Enzyme activities: a Comparative Investigation, Genetic Toxicology and Environmental Mutagenesis, 515, 111–124, 200 Dubovskiy I.M., Grizanova E.V., Ershova N.S., Rantala M.J., Glupov V.V., The Effects of Dietary Nickel on The Detoxification Enzymes, Innate Immunity and Resistance to The Fungus Beauveria bassiana in The Larvae of The Greater Wax Moth Galleria mellonella, Chemosphere, 85, 92-96, 2011.
  • Shen G., Lu Y., Zhou Q., Hong J., Interaction of Polycyclic Aromatic Hydrocarbons and Heavy Metals on Soil Enzyme, Chemosphere, 61, 1175–1182, 2005.
  • Xu H., Song P., Gu W., Yang Z., Effects of Heavy Metals on Production of Thiol Compounds and Antioxidant Enzymes in Agaricus bisporus, Ecotoxicology and Environmental Safety, 74, 1685-1692, 2011.
  • Kazımírova M., Slovák M., Effects of Heavy Metals and Fluorine on Phagocytosis and Phenoloxidase Activity in Mamestra brassicae, Eur. J. Entomol., 93, 467-473, 1996.
  • Gianfreda L., Ruggiero P., Soil Biology, Springer-Verlag Berlin Heidelberg, 257-311, 200 Nayeka S., Guptaa S., Sahab R.N., Metal Accumulation and Its Effects in Relation to Biochemical Response of Vegetables Irrigated with Metal Contaminated Water and Wastewater, Journal of Hazardous Materials, 178, 588–595, 20 Anjum N.A., Ahmad I., Mohmood I., Pacheco M., Duarte A.C., Pereira E., Umar S., Ahmad A., Khan N.A., Iqbal M., Prasad M.N.V., Modulation of Glutathione and Its Related Enzymes in Plants’ Responses to Toxic Metals and Metalloids, Environmental and Experimental Botany, 75, 307–324, 2012.
  • Saffar A., Najjar M.B.B., Mianabadi M., Activity of Antioxidant Enzymes in Response to Cadmium in Arabidopsis thaliana, Journal of Biological Sciences, 9 (1), 44-50, 2009.
  • Israr M., Jewella A., Kumarb D., Sahia S.V., Interactive Effects of Lead, Copper, Nickel and Zinc on Growth, Metal Uptake and Antioxidative Metabolism of Sesbania drummondii, Journal of Hazardous Materials, 186, 1520–1526, 2011.
  • Jaleel C.A., Jayakumar K., Chang-Xing Z., Azooz M.M., Effect of Soil Applied Cobalt on Activities of Antioxidant Enzymes in Arachis hypogaea, Global Journal of Molecular Sciences, 3 (2), 42-45, 2008.
  • Constabel C.P., Barbehenn R., Induced Plant Resistance to Herbivory, Springer, Dordrecht , 253-269, 2008.
  • Karagöz A., Cevahir G., Özcan T., Sadıkoğlu N., Yentür S., Kuru A., Bazı Yüksek Bitkilerden Hazırlanan Sulu Ekstrelerin Antiviral Aktivite Potansiyellerinin Değerlendirilmesi, Bitkisel İlaç Hammaddeleri Toplantısı-Bildiriler, Eskişehir, 318-321, 2002.
  • Akçin Ö.E., Kandemir N., Akçin Y., A Morphological and Anatomical Study on a Medicinal and Edible Plant Trachystemon orientalis (L.) G.Don (Boraginaceae) in the Black Sea Region, Turk J Bot, 28, 435-442, 200 Özen T., Antioxidant Activity of Wild Edible Plants in The Black Sea Region of Turkey, Grasas y Aceıtes, 61 (1), 86-94, 2010.
  • Ceyhun A.B., Şentürk M., Yerlikaya E., Erdoğan O., Küfrevioğlu Ö.İ., Ekinci D., Purification and Characterization of Carbonic Anhydrase from The Teleost Fish Dicentrarchus labrax (European seabass) Liver and Toxicological Effects of Metals on Enzyme Activity, Environmental Toxicology and Pharmacology, 32, 69-74, 2011.
  • Güngör K., Çağla Badem (Prunus dulcis) Bitkisinden Polifenol Oksidaz Enziminin Saflaştırılması ve Karakterizasyonu, Yüksek Lisans Tezi, Sakarya Üniversitesi Fen Bilimleri Enstitüsü, Sakarya, 2008.
  • Gao Z.J., Liu J.B., Xiao X.G., Purification and Characterisation of Polyphenol Oxidase from Leaves of Cleome gynandra L., Food Chemistry, 129, 1012-1018, 2011.
  • Yang C.P., Fujita S., Ashrafuzzaman M.D., Nakamura N., Hayashi N., Purification and Characterization of Polyphenol Oxidase from Banana (Musa sapientum L.) Pulp, J. Agric. Food Chem., 48, 2732-2735, 2000.
  • Aydemir T., Selected Kinetic Properties of Polyphenol Oxidase Extracted from Rosmarinus Officinalis L., International Journal of Food Properties, 13 (3), 475-485, 2010.
  • Gao Z.J., Han X.H., Xiao X.G., Purification and Characterisation of Polyphenol Oxidase from Red Swiss Chard (Beta vulgaris subspecies cicla) Leaves, Food Chemistry, 117, 342-348, 200
There are 20 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Research Articles
Authors

Esma Hande Alıcı This is me

Gülnur Arabacı This is me

Publication Date June 1, 2013
Submission Date January 18, 2013
Acceptance Date August 1, 2013
Published in Issue Year 2013 Volume: 17 Issue: 3

Cite

APA Alıcı, E. H., & Arabacı, G. (2013). Kaldirik(Trachystemon orientalis) bitkisi Polifenol oksidaz enzimi üzerine metallerin etkisi. Sakarya University Journal of Science, 17(3), 399-405. https://doi.org/10.16984/saufbed.99926
AMA Alıcı EH, Arabacı G. Kaldirik(Trachystemon orientalis) bitkisi Polifenol oksidaz enzimi üzerine metallerin etkisi. SAUJS. December 2013;17(3):399-405. doi:10.16984/saufbed.99926
Chicago Alıcı, Esma Hande, and Gülnur Arabacı. “Kaldirik(Trachystemon Orientalis) Bitkisi Polifenol Oksidaz Enzimi üzerine Metallerin Etkisi”. Sakarya University Journal of Science 17, no. 3 (December 2013): 399-405. https://doi.org/10.16984/saufbed.99926.
EndNote Alıcı EH, Arabacı G (December 1, 2013) Kaldirik(Trachystemon orientalis) bitkisi Polifenol oksidaz enzimi üzerine metallerin etkisi. Sakarya University Journal of Science 17 3 399–405.
IEEE E. H. Alıcı and G. Arabacı, “Kaldirik(Trachystemon orientalis) bitkisi Polifenol oksidaz enzimi üzerine metallerin etkisi”, SAUJS, vol. 17, no. 3, pp. 399–405, 2013, doi: 10.16984/saufbed.99926.
ISNAD Alıcı, Esma Hande - Arabacı, Gülnur. “Kaldirik(Trachystemon Orientalis) Bitkisi Polifenol Oksidaz Enzimi üzerine Metallerin Etkisi”. Sakarya University Journal of Science 17/3 (December 2013), 399-405. https://doi.org/10.16984/saufbed.99926.
JAMA Alıcı EH, Arabacı G. Kaldirik(Trachystemon orientalis) bitkisi Polifenol oksidaz enzimi üzerine metallerin etkisi. SAUJS. 2013;17:399–405.
MLA Alıcı, Esma Hande and Gülnur Arabacı. “Kaldirik(Trachystemon Orientalis) Bitkisi Polifenol Oksidaz Enzimi üzerine Metallerin Etkisi”. Sakarya University Journal of Science, vol. 17, no. 3, 2013, pp. 399-05, doi:10.16984/saufbed.99926.
Vancouver Alıcı EH, Arabacı G. Kaldirik(Trachystemon orientalis) bitkisi Polifenol oksidaz enzimi üzerine metallerin etkisi. SAUJS. 2013;17(3):399-405.