The metal effect on the Polyphenol oxidase enzyme from borage(Trachystemon orientalis) plant

Year 2013, Volume: 17 Issue: 3, 399 - 405, 01.06.2013

Esma Hande Alıcı Gülnur Arabacı

Abstract

In this study, Polyphenol oxidase was extracted from Borage plant (Trachystemon orientalis) and the crude extract was used for the assays. Its pH and temperature optima for 4-methyl cathecol were 5.0 and 5° C, respectively. Km of this enzyme was 4.55 mM for 4-methyl cathecol. We also found that the enzyme was activated by Fe3+ , Mg 2+ , Zn 2+ , Cu 2+ , Ca 2+ , K + , but inhibited by Hg2+ , Mn 2+ , Ni 2+ , Sn 2+ , Na + . Ba 2+ , Al 3+ and Pb2+ metal ions both activated and inhibited the enzyme at different concentrations.

Keywords

Trachystemon orientalis, Polyphenol oxidase, metal effect, enzyme kinetics

Kaldirik(Trachystemon orientalis) bitkisi Polifenol oksidaz enzimi üzerine metallerin etkisi

Abstract

In this study, Polyphenol oxidase was extracted from Borage plant (Trachystemon orientalis) and the crude extract was used for the assays. Its pH and temperature optima for 4-methyl cathecol were 5.0 and 5°C, respectively. Km of this enzyme was 4.55 mM for 4-methyl cathecol. We also found that the enzyme was activated by Fe3+, Mg2+, Zn2+, Cu2+, Ca2+, K+, but inhibited by Hg2+, Mn2+, Ni2+, Sn2+, Na+. Ba2+, Al3+ and Pb2+ metal ions both activated and inhibited the enzyme at different concentrations.

Keywords

Trachystemon orientalis, Polifenol oksidaz, metal etkisi, enzim kinetiği

References

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