Detection Of Genotoxicity Induced By Heavy Metal Ions And Gamma Radiation Using Micronucleus Assay In Mice: Pathological Evaluation

Year 2010, Volume: 14 Issue: 1, 1 - 8, 01.06.2010

Kültiğin Çavuşoğlu Emine Yalçın Oğuz Kul Abdullah Yılmaz Kürşad Yapar

Abstract

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Keywords

Detection Of Genotoxicity Induced By Heavy Metal Ions And Gamma Radiation Using Micronucleus Assay In Mice: Pathological EvaluationKültiğin Çavuşoğlu1, Emine Yalçın1, Oğuz Kul2, Abdullah Yılmaz3, Kürşad Yapar41Department Of Biology, Faculty Of Science A

Farelerde Mikronukleus Testi Kullanılarak Gama Radyasyonu Ve Ağır Metal İyonları Tarafindan Teşvik Edilen Genotoksisitenin Belirlenmesi: Patolojik Değerlendirmeli

Abstract

Micronuclei (MN) test is used as markers of radiosensitivity or chemosensitivity. In present study, it was investigated the frequency of MN in erythrocytes and body weight gain in 80 Mus musculus var. albinos exposed to 10 Gy gamma (γ) radiation and heavy metal ions. For this aim, it was used MN assay as an indicator of genotoxicity induced by γradiation and heavy metal toxication. The animals were divided into four groups: control, radiation, Hg and Pb treatment groups. They were treated with three dose levels (10, 15 and 20 µg/mL) of Hg and Pb metal ions and 10 Gy γradiaton was applied twice during 14 days. The initial and final weights of all mice were determined by sensitive balance in order to investigate the effect of heavy metal ions and radiation on the weight gain of mice. As a result, the frequency of MN was higher in the Hg, Pb and γradiation treated animals than animals in control group. Besides, MN frequency was higher in mice exposed to γradiation than in Hg and Pb treated mice, and differences was statistically significant (p

Keywords

Farelerde Mikronukleus Testi Kullanılarak Gama Radyasyonu Ve Ağır Metal İyonları Tarafindan Teşvik Edilen Genotoksisitenin Belirlenmesi: Patolojik DeğerlendirmeliKültiğin Çavuşoğlu1, Emine Yalçın1, Oğuz Kul2, Abdullah Yılmaz3, Kürşad Yapar41Giresun Üniv

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