Flow cytometry and its uses

Year 2016, Volume: 20 Issue: 1, 33 - 38, 01.04.2016

Martin Kanev , Fulya Gökalp Muranlı

https://doi.org/10.16984/saufenbilder.45424

Cited By: 3

Abstract

Response of cells to be detected using biochemical and physical properties of the microscope for many years the primary specifications have been used for flow cytometry techniques. Flow cytometry is a technique and apparatus based on a single pass along the flow channel and the basis for classification based on cell size and granularity in this order as a suspension of various cells. The surface and internal proteins of cells with this equipment, organelles and other components analysis and separation, size using laser and electronic technology are performed by the granularity and fluorescence emission as basis. Flow cytometry is the basis of technical know well this technique will increase the diagnostic value of standardization and allowing us to use the results in many pathologies.

Keywords

Flow cytometry, cell analysis, cell sorting, usage areas

Flow sitometri ve kullanım alanları

Abstract

Hücrelerin biyokimyasal ve fiziksel özelliklerinin senelerdir primer mikroskop özellikleri kullanılarak saptanmasına karşılık, günümüzde flow sitometri tekniği kullanılmaya başlanmıştır. Flow sitometri, çeşitli hücrelerin bir süspansiyon halinde bir akış kanalı boyunca tek tek geçmesi ve bu sırada hücre büyüklüğü ve granülaritesine göre sınıflandırılması esasına dayanan bir teknik ve cihazdır. Bu cihaz ile hücrenin yüzey ve iç proteinleri, organelleri ve diğer bileşenleri analiz ve ayrımı, lazer ve elektronik teknolojisi kullanılarak büyüklük, granülarite ve floresans emisyonu esasına göre gerçekleştirilmektedir. Flow sitometri tekniğinin temelini iyi bilmemiz bu tekniği birçok patolojide kullanmamızı sağlayarak sonuçlarının standardizasyonunu ve tanı koydurucu değerini de arttıracaktır.

Keywords

Flow sitometri, hücre analizi, hücre gruplama, kullanım alanları

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