Topraktan izole edilen Bacillus türlerinin tanımlanması ve biyolojik özelliklerinin araştırılması

Year 2016, Volume: 20 Issue: 2, 281 - 290, 01.08.2016

Hatice Katı Burak Karaca Şebnem Hazal Gülşen

https://doi.org/10.16984/saufenbilder.04840

Cited By: 2

Abstract

Bu çalışmada, Giresun adasından toplanan toprak örneklerinden Bacillus izolasyonu yapılmıştır. Bu izolatlar morfolojik, biyokimyasal ve moleküler olarak tanımlanmıştır. Tanımlanan Bacillus’larda bazı ekstrasellüler enzimlerinin varlığı kalitatif olarak incelenmiştir. Ayrıca Bacillus izolatlarının bazı bakterilere karşı antibakteriyal aktiviteleri agar difüzyon metoduna göre araştırılmıştır. Sonuç olarak 38 izolat B. cereus grubu üyesi, 7 izolat B. thuringiensis, 10 izolat B. megaterium, 6 izolat B. pumilus ve 12 izolat Bacillus sp. olarak tanımlanmıştır. Bacillus izolatlarının ekstrasellüler enzim aktivite sonuçları değerlendirildiğinde 38 izolatın amilaz, 53 izolatın lipaz/esteraz, 16 izolatın kitinaz, 7 izolatın ksilanaz, 2 izolatın pektinaz, 73 izolatın proteaz ve 35 izolatın selülaz enzim aktivitesi pozitif olarak bulunmuştur. Çalışmada kullanılan Bacillus izolatları test edilen mikroorganizmaların gelişmelerini değişik oranlarda engellemiştir. Dokuz izolat yüksek antibakteriyal aktivite göstermiştir. 

Keywords

Antimikrobiyal madde, Bacillus, Bacillus thuringiensis, Enzim

Identification of Bacillus species isolated from soil and investigation of their biological properties

Abstract

In this study, Bacillus isolation were performed from soil samples collected from Giresun Island. These isolates were characterized as morphological, biochemical, and molecular. The presence of some extracellular enzymes in the identified Bacillus isolates were qualitatively investigated. Furthermore the antibacterial activities of the Bacillus isolates against some bacteria were examined by the agar diffusion method. As a result 38 isolates were identified as the members of B. cereus group, 7 as B. thuringiensis, 10 as B. megaterium, 6 as B. pumilus, and 12 as Bacillus sp. When the extracellular enzyme activity results of the identified Bacillus isolates were evaluated, it was found that 38 of these have amylase, 53 have lipase/esterase, 16 have chitinase, 7 have xylanase, 2 have pectinase, 73 have protease, and 35 have cellulase positive enzyme activities. The Bacillus isolates used in this study inhibited the growth of tested microorganisms in varying ratios. Nine isolates show high antibacterial activity.

Keywords

antibacterial activity, Bacillus, Bacillus thuringiensis, extracellular enzyme

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