Dodin fungusitinin Vicia faba L. bitkisinde total protein miktarı ve peroksidaz aktivitesi üzerine etkisi

Year 2016, Volume: 20 Issue: 3, 627 - 633, 11.10.2016

Nurşen Çördük Nihan Akıncı Nergis Kaya Gülru Yücel Cüneyt Akı

https://doi.org/10.16984/saufenbilder.22241

Cited By: 2

Abstract

Bu araştırmada dodin fungusitinin Vicia faba L. fizyolojisi üzerindeki etkisi, total protein miktarı ve peroksidaz (POX) aktivitesi aracılığıyla değerlendirilmiştir. Bu doğrultuda, dört haftalık V. faba fideleri 0.04 mL/L, 0.08 mL/L ve 0.16 mL/L’lik dodin konsantrasyonları ile muamele edilmiştir. Bu işlemlerden sonra bitki örneklerinin yaprakları 24, 48 ve 72 saat ara ile hasat edilmiştir. Yapılan analizler sonucunda, artan dodin konsantrasyonu ve muamele süresiyle total protein miktarı kontrole göre önemli ölçüde azalırken, peroksidaz aktivitesi yükselmiştir. Elde edilen sonuçlar dodinin bitki savunma sistemini uyardığını göstermiştir.

 

Keywords

1-dodesil guanidium asetat, enzim aktivitesi, Vicia faba, pestisit, total protein

Effects of dodine on total protein content and peroxidase activity in Vicia faba L.

Abstract

This research was to evaluate the effects of dodine on the physiology of Vicia faba L. through total protein content and peroxidase (POX) activity. According to this, four weeks old seedlings were treated with 0.04 mL/L, 0.08 mL/L and 0.16 mL/L concentrations of dodine. Then, the leaves of plant samples were harvested with 24, 48 and 72 hours intervals. Our results have showed that while the total protein content were decreased significantly, POX activity was increased with the increased concentration of dodine and exposure time when compared with control seedlings. This research have shown that dodine can stimulate the plant defense system.

Keywords

dodecylguanidinium acetate, enzyme activity, Vicia faba, pesticide, total protein

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