An Investigation of Inhibition Effects of Honey, Pollen, Propolis and Royal Jelly Extracts on Thioredoxin Reductase Enzyme Activity

Year 2018, Volume: 22 Issue: 6, 1585 - 1590, 01.12.2018

Ebru Akkemik , Gamze Akbulut

https://doi.org/10.16984/saufenbilder.341372

Cited By: 1

Abstract

The thioredoxin reductase
enzyme is an enzyme that prevents the mechanism of apoptosis from working and
thus triggers the formation of cancer. Therefore, the inhibition of the
thioredoxin reductase enzyme is thought to prevent or inhibit cancer. In this
study, the effects of extracts of plateau honey, pine honey, chestnut honey,
mad or wild honey, pollen, propolis and royal jelly on thioredoxin reductase
enzyme activity were investigated. Enzyme activities were measured at constant
substrate and different inhibitor concentrations to calculate IC₅₀
values. Over five different inhibitor concentrations were used for measuring
the inhibition constant. Total antioxidant activity were investigated in order
to compare the extracts used in the inhibition study. The strongest inhibitory
effect was seen in the pollen methanol extract (IC₅₀ = 2.44μg /mL)

Keywords

bee products, Enzyme Activity, Honey

Tiyoredoksin Redüktaz Enzim Aktivitesi Üzerine Bal, Polen, Propolis ve Arı Sütü Extraktlarının İnhibisyon Etkilerinin Araştırılması

Abstract

Tiyoredoksin redüktaz enzimi,
apoptoz mekanizmasının çalışmasını önleyen ve böylece kanser oluşumunu
tetikleyen bir enzimdir. Bu nedenle tiyoredoksin redüktaz enziminin
inhibisyonunun kanseri önlediği veya durdurduğu düşünülmektedir. Bu çalışmada, yayla
balının, çam balının, kestane balının, deli veya yabani balının, polenin,
propolisin ve arı sütünün ekstraktlarının tioredoksin redüktaz enzim aktivitesi
üzerindeki etkileri araştırılmıştır. Enzim aktiviteleri IC₅₀ değerlerini
hesaplamak için sabit substrat ve farklı inhibitör konsantrasyonlarında ölçülmüştür.
İnhibisyon sabitinin ölçümü için beşten fazla farklı inhibitör
konsantrasyonları kullanılmıştır. İnhibisyon çalışmasında kullanılan
ekstraktları karşılaştırmak için toplam antioksidan aktivitesi araştırılmıştır.
En güçlü inhibisyon etkisi polen metanol ekstraktında (IC₅₀ = 2.44
μg/mL) görülmüştür.

Keywords

kanser, arı ürünleri

References

• [1] Anonymous, “Yeni Dünya Kanser İstatistikleri Yayınlandı,” 2017. [Online]. Available: http://kanser.gov.tr/daire-faaliyetleri/kanser-istatistikleri/860-yeni-dünya-kanser-istatistikleri-yayınlandı.html. [Accessed: 05-Aug-2017].
• [2] B. Tandogan and N. N. Ulusu, “Thioredoxin Reductase,” Hacettepe J. Biol. Chem., vol. 39, no. 1, pp. 87–92, 2011.
• [3] J. Lu, E.-H. Chew, and A. Holmgren, “Targeting thioredoxin reductase is a basis for cancer therapy by arsenic trioxide,” Proc. Natl. Acad. Sci., vol. 104, no. 30, pp. 12288–12293, Jul. 2007.
• [4] D. Mustacich and G. Powis, “Thioredoxin reductase.,” Biochem. J., vol. 346 Pt 1, pp. 1–8, Feb. 2000.
• [5] A. Jordan and P. Reichard, “Ribonucleotide reductases.,” Annu. Rev. Biochem., vol. 67, pp. 71–98, 1998.
• [6] M. Cox and D. L. Nelson, Lehninger Principles of Biochemistry, 5th ed. New York: W.H. Freeman and Company., 2008.
• [7] J. W. White, “Composition of Honey,” in Honey: A Comprehensive Survey, E. Crane, Ed. London: Heinemann, 1975, pp. 157–206.
• [8] A. M. Gómez-Caravaca, M. Gómez-Romero, D. Arráez-Román, A. Segura-Carretero, and A. Fernández-Gutiérrez, “Advances in the analysis of phenolic compounds in products derived from bees,” J. Pharm. Biomed. Anal., vol. 41, no. 4, pp. 1220–1234, Jun. 2006.
• [9] M. Ahn, S. Kumazawa, Y. Usui, J. Nakamura, M. Matsuka, F. Zhu, and T. Nakayama, “Antioxidant Activity and Constituents of Propolis Collected in Various Areas of Korea,” J. Agric. Food Chem., vol. 52, no. 4, pp. 7286–7292, 2004.
• [10] M. Küçük, S. Kolaylı, Ş. Karaoğlu, E. Ulusoy, C. Baltacı, and F. Candan, “Biological activities and chemical composition of three honeys of different types from Anatolia,” Food Chem., vol. 100, no. 2, pp. 526–534, Jan. 2007.
• [11] F. Karadal and Y. Yildirim, “Balın Kalite Nitelikleri, Beslenme ve Sağlık Açısından Önemi,” Erciyes Üniv Vet Fak Derg YILDIRIM Erciyes Üniv Vet Fak Derg Derlemeler J Fac Vet Med Univ Erciyes, vol. 9, no. 93, pp. 197–209, 2012.
• [12] Anonymous, “Bal Çeşitleri,” 2017. [Online]. Available: http://apiterapi.uzerine.com/index.jsp?objid=340,. [Accessed: 05-Aug-2017].
• [13] S. Kolayli, Z. Can, O. Yildiz, H. Sahin, and S. A. Karaoglu, “A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut ( Castanea sativa Mill. ) honeys,” J. Enzyme Inhib. Med. Chem., vol. 6366, no. March 2017, pp. 1–9, 2016.
• [14] N. Sütlüpmar, A. Mat, and Y. Satganoglu, “Poisoning by toxic honey in Turkey,” Arch. Toxicol., vol. 67, no. 2, pp. 148–150, 1993.
• [15] O. Yilmaz, “Honey Bee Products in Turkey,” J. Anim. Sci. Adv., vol. 6, no. 10, p. 1779, 2016.
• [16] M. Marcucci, “Propolis : chemical composition , biological properties and therapeutic activity Mc Marcucci Propolis : chemical composition , biological properties and therapeutic activity,” 1995.
• [17] Z. Selamoglu Talas, “Propolis reduces oxidative stress in l-NAME-induced hypertension rats,” Cell Biochem. Funct., vol. 32, no. 2, pp. 150–154, 2014.
• [18] T. Nagai and R. Inoue, “Preparation and the functional properties of water extract and alkaline extract of royal jelly,” Food Chem., vol. 84, no. 2, pp. 181–186, 2004.
• [19] H. Sahin, R. Aliyazicioglu, O. Yildiz, S. Kolayli, a Innocenti, and C. T. Supuran, “Honey, polen, and propolis extracts show potent inhibitory activity against the zinc metalloenzyme carbonic anhydrase.,” J. Enzyme Inhib. Med. Chem., vol. 26, no. 3, pp. 440–444, 2011.
• [20] A. Holmgren, “Bovine thioredoxin system. Purification of thioredoxin reductase from calf liver and thymus and studies of its function in disulfide reduction.,” J. Biol. Chem., vol. 252, no. 13, pp. 4600–6, Jul. 1977.
• [21] F. Odabasoglu, A. Aslan, A. Cakir, H. Suleyman, Y. Karagoz, M. Halici, and Y. Bayir, “Comparison of antioxidant activity and phenolic content of three lichen species,” Phyther. Res., vol. 18, no. 11, pp. 938–941, Nov. 2004.
• [22] M. Saitoh, H. Nishitoh, M. Fujii, K. Takeda, K. Tobiume, Y. Sawada, M. Kawabata, K. Miyazono, and H. Ichijo, “Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1,” EMBO J., vol. 17, no. 9, pp. 2596–2606, 1998.
• [23] J. Fang, J. Lu, and A. Holmgren, “Thioredoxin reductase is irreversibly modified by curcumin: a novel molecular mechanism for its anticancer activity.,” J. Biol. Chem., vol. 280, no. 26, pp. 25284–90, Jul. 2005.
• [24] P. B. Cassidy, K. Edes, C. C. Nelson, K. Parsawar, F. A. Fitzpatrick, and P. J. Moos, “Thioredoxin reductase is required for the inactivation of tumor suppressor p53 and for apoptosis induced by endogenous electrophiles,” Carcinogenesis, vol. 27, no. 12, pp. 2538–2549, Aug. 2006.
• [25] K. Becker, S. Gromer, R. H. Schirmer, and S. Müller, “Thioredoxin reductase as a pathophysiological factor and drug target.,” Eur. J. Biochem., vol. 267, no. 20, pp. 6118–25, Oct. 2000.
• [26] M. Marcucci, F. Ferreres, C. García-Viguera, V. S. Bankova, S. L. De Castro, A. P. Dantas, P. H. Valente, and N. Paulino, “Phenolic compounds from Brazilian propolis with pharmacological activities.,” J. Ethnopharmacol., vol. 74, no. 2, pp. 105–12, Feb. 2001.
• [27] K. Pyrzynska and M. Biesaga, “Analysis of phenolic acids and flavonoids in honey,” TrAC Trends Anal. Chem., vol. 28, no. 7, pp. 893–902, Aug. 2009.
• [28] B. W. LeBlanc, O. K. Davis, S. Boue, A. DeLucca, and T. Deeby, “Antioxidant activity of Sonoran Desert bee pollen,” Food Chem., vol. 115, no. 4, pp. 1299–1305, Aug. 2009.