Comparative Evaluation of Alginate-Gelatin Hydrogel, Cryogel, and Aerogel Beads as a Tissue Scaffold

Year 2023, Volume: 27 Issue: 2, 335 - 348, 30.04.2023

Ece Bayır

https://doi.org/10.16984/saufenbilder.1098637

Cited By: 1

Abstract

Hydrogels are frequently used in tissue engineering and regenerative medicine, drug delivery, and environmental remediation. Alginate and gelatin, which are frequently used natural polymers to form hydrogels, were chosen in this study to form a core-shell structured hydrogel. Cryogels and aerogels were obtained by drying hydrogels with different methods, freeze-drying, and the continuous flow of supercritical CO2, respectively. The potential use of hydrogels, aerogels, and cryogels as a tissue scaffold was evaluated comparatively. Characterizations of materials were determined morphologically by scanning electron microscope and computed-micro tomography, chemically by energy dispersive spectroscopy, and mechanically by the dynamic mechanical analyzer. In addition, the cytotoxic effect of all structures was analyzed by the WST-1 method and the localization of the cells in these structures was determined by microscopic methods. All scaffolds show non-cytotoxic effects. Cryogels have the highest porosity (85.21 %) and mean pore size values (62.3±26.8 µm). Additionally, cryogels show high water retention capacity (782±53.5%) than aerogels (389±2.5%) for 24 h. The elastic modulus values were <10 kPa, which is suitable for brain, bone marrow, spleen, pancreas, fat, kidney, and skin tissue engineering, for all types of beads. It has been determined that cryogel and hydrogel beads are more suitable for cell adhesion and migration in this study.

Keywords

Tissue engineering, scaffold, sodium alginate, gelatin

Thanks

I thank Ege-MATAL and Prof. Dr. Suna Timur for laboratory facilities, and Dr. Raif İlktaç for his help.

References

• [1] R. Parhi, "Cross-Linked Hydrogel for Pharmaceutical Applications: A Review," Advanced pharmaceutical bulletin, vol. 7, no. 4, pp. 515-530, 2017.
• [2] M. F. Akhtar, M. Hanif, N. M. Ranjha, "Methods of synthesis of hydrogels … A review," Saudi Pharmaceutical Journal, vol. 24, no. 5, pp. 554-559, 2016.
• [3] I. M. El-Sherbiny, M. H. Yacoub, "Hydrogel scaffolds for tissue engineering: Progress and challenges," Global Cardiology Science and Practice, p. 38, 2013.
• [4] A. C. Daly, L. Riley, T. Segura, J. A. Burdick, "Hydrogel microparticles for biomedical applications," Nature Reviews Materials, vol. 5, no. 1, pp. 20-43, 2020.
• [5] C. A. Dreiss, "Hydrogel design strategies for drug delivery," Current Opinion in Colloid & Interface Science, vol. 48, pp. 1-17, 2020.
• [6] X. Qi, L. Wu, T. Su, J. Zhang, W. Dong, "Polysaccharide-based cationic hydrogels for dye adsorption," Colloids and Surfaces B: Biointerfaces, vol. 170, pp. 364-372, 2018.
• [7] C. D. Spicer, "Hydrogel scaffolds for tissue engineering: the importance of polymer choice," Polymer Chemistry, vol. 11, no. 2, pp. 184-219, 2020.
• [8] V. Van Tran, D. Park, Y. C. Lee, "Hydrogel applications for adsorption of contaminants in water and wastewater treatment," Environmental Science and Pollution Research, vol. 25, no. 25, pp. 24569-24599, 2018.
• [9] W. Jiao, W. Chen, Y Mei, Y. Yun, B. Wang, Q. Zhong, H. Chen, W. Chen "Effects of Molecular Weight and Guluronic Acid/Mannuronic Acid Ratio on the Rheological Behavior and Stabilizing Property of Sodium Alginate," Molecules, vol. 24, no. 23, 2019.
• [10] F. Abasalizadeh, S. V. Moghaddam, E. Alizadeh, E. Akbari, E. Kashani, S. M. B. Fazljou, M. Torbati, A. Akbarzadeh, "Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting," Journal of biological engineering, vol. 14, no. 8, pp. 1-22, 2020.
• [11] P. Jaipan, A. Nguyen, R. J. Narayan, "Gelatin-based hydrogels for biomedical applications," MRS Communications, vol. 7, no. 3, pp. 416-426, 2017.
• [12] S. Afewerki, A. Sheikhi, S. Kannan, S. Ahadian, A. Khademhosseini, "Gelatin-polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics," Bioengineering and Translational Medicine, vol. 4, no. 1, pp. 96-115, 2018.
• [13] D. Chawla, T. Kaur, A. Joshi, N. Singh, “3D bioprinted alginate-gelatin based scaffolds for soft tissue engineering,” International Journal of Biological Macromolecules, vol. 144, pp. 560-567, 2019.
• [14] T. Pan, W. Song, X. Cao, Y. Wang, “3D bioplotting of gelatin/alginate scaffolds for tissue engineering: influence of crosslinking degree and pore architecture on physicochemical properties,” Journal of Materials Science & Technology, vol. 32, no. 9, pp. 889-900, 2016.
• [15] L. Baldino, S. Cardea, E. J. C. E. T. Reverchon, “Natural aerogels production by supercritical gel drying,” Chemical Engineering Transactions, vol. 43, pp. 739-744, 2015.
• [16] R. Rodríguez-Dorado, C. López-Iglesias, C. A. García-González, G. Auriemma, R. P. Aquino, P. Del Gaudio, "Design of aerogels, cryogels and xerogels of alginate: Effect of molecular weight, gelation conditions and drying method on particles’ micromeritics," Molecules, vol. 24, no. 6, p. 1049, 2019.
• [17] L. Baruch, M. Machluf, “Alginate–chitosan complex coacervation for cell encapsulation: Effect on mechanical properties and on long‐term viability,” Biopolymers: Original research on biomolecules, vol. 82, no. 6, pp. 570-579, 2006.
• [18] Biological evaluation of medical devices, 10993-5, ISO, 2009.
• [19] W. Y. Leong, C. F. Soon, S. C. Wong, K. S. Tee, S. C. Cheong, S. H. Gan, M. Youseffi, “In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique,” Bioengineering, vol. 4.2, no. 43, pp. 1-14, 2017.
• [20] C. S. Bento, S. Alarico, N. Empadinhas, H. C. de Sousa, M. E. Braga, "Sequential scCO2 Drying and Sterilisation of Alginate-Gelatine Aerogels for Biomedical Applications," The Journal of Supercritical Fluids, p. 105570, 2022.
• [21] Q. Chen, X. Tian, J. Fan, H. Tong, Q. Ao, X. Wang, "An interpenetrating alginate/gelatin network for three-dimensional (3D) cell cultures and organ bioprinting," Molecules, vol. 25, no. 3, p. 756, 2020.
• [22] C. M. Murphy, A. Matsiko, M. G. Haugh, J. P. Gleeson, F. J. O’Brien, “Mesenchymal stem cell fate is regulated by the composition and mechanical properties of collagen–glycosaminoglycan scaffolds,” Journal of the mechanical behavior of biomedical materials, vol. 11, pp 53-62, 2012.
• [23] A. M. Handorf, Y. Zhou, M. A. Halanski, W.-J. Li, "Tissue stiffness dictates development, homeostasis, and disease progression," Organogenesis, vol. 11, no. 1, pp. 1-15, 2015.
• [24] J. Liu, H. Zheng, P. S. Poh, H.-G. Machens, A. F. Schilling, "Hydrogels for engineering of perfusable vascular networks," International journal of molecular sciences, vol. 16, no. 7, pp. 15997-16016, 2015.
• [25] A. Barros, S. Quraishi, M. Martins, P. Gurikov, R. Subrahmanyam, I. Smirnova, A. R. C. Duarte, R. L. Reis, "Hybrid Alginate‐Based Cryogels for Life Science Applications," Chemie Ingenieur Technik, vol. 88, no. 11, pp. 1770-1778, 2016.
• [26] T. P. Nguyen, B. T. Lee, "Fabrication of oxidized alginate-gelatin-BCP hydrogels and evaluation of the microstructure, material properties and biocompatibility for bone tissue regeneration," Journal of Biomaterials Applications, vol. 27, no. 3, pp. 311-21, 2012.
• [27] L. Yuan, Y. Wu, J. Fang, X. Wei, Q. Gu, H. El-Hamshary, S. S. Al-Deyab, Y. Morsi, X. Mo, "Modified alginate and gelatin cross-linked hydrogels for soft tissue adhesive," Artificial cells, nanomedicine, and biotechnology, vol. 45, no. 1, pp. 76-83, 2017.
• [28] F. Dehghani, N. Annabi, “Engineering porous scaffolds using gas-based techniques,” Current opinion in biotechnology, vol. 22, no. 5, pp. 661-666, 2011.
• [29] W. Aljohani, L. Wenchao, M. Ullah, X. Zhang, G. Yang, "Application of sodium alginate hydrogel,", vol. 3, no. 3, pp. 19-31, 2017.