Bioaccumulation of Nickel Ions by Rhizopus delemar

Year 2025, Volume: 29 Issue: 2, 208 - 217

Gülşah Başkan , Unsal Açıkel

https://doi.org/10.16984/saufenbilder.1356825

Abstract

The current research investigated the bioaccumulation of Ni(II) ions by Rhizopus delemar in molasses-containing fluids in a batch reactor. Due to the low pH requirements that R. delemar requires, it may grow in wastewater, which has an acidic pH. In the absence of Ni(II) ions, the influence of pH and molasses concentration on the growth rate and concentration of R. delemar were examined. The highest level of microbial growth occurred at a pH of 4.0. Up to 20 g/L of sucrose content increased the maximum R. delemar concentration and the specific growth rate. While the substrate content in each growing medium including molasses was kept constant at 10 g/L, initial concentrations of metal ions were changed between 50 and 250 mg/L to evaluate the bioaccumulation of Ni(II) ions. It was discovered that when metal ions existed, the rate of microorganism growth slowed down as the metal ion concentration increased. The maximum growth rates were discovered to be 0.257 h-1 in the presence of 50 mg/L Ni(II). When media containing 50 mg/L Ni(II) ions, the efficiency of Ni(II) bioaccumulation was found to be 51.8%.

Keywords

Rhizopus delemar, Bioaccumulation, Nickel, Microbial growth, Molasses sucrose

References

• N. A. A. Qasem, R. H. Mohammed, D. U. Lawal, “Removal of heavy metal ions from wastewater: A comprehensive and critical review”, NPJ Clean Water, vol. 4, no. 36, 2021.
• Renu, M. Agarwal, K. Singh, “Heavy metal removal from wastewater using various adsorbents: A review”, Journal of Water Reuse and Desalination, vol. 7, no. 4, pp. 387-419, 2017.
• E. Priyadarshini, S. S. Priyadarshini, B. G. Cousins, N. Pradhan, “Metal-Fungus interaction: Review on cellular processes underlying heavy metal detoxification and synthesis of metal nanoparticles”, Chemosphere, vol. 274, 2021.
• R. Shrestha, S. Ban, S. Devkota, S. Sharma, R. Joshi, A. P. Tiwari, H. Y. Kim, M. K. Joshi, “Technological trends in heavy metals removal from industrial wastewater: A review”, Journal of Environmental Chemical Engineering, vol. 9, no. 4, 2021.
• P. Diep, R. Mahadevan, A. F. Yakunin, “Heavy metal removal by bioaccumulation using genetically engineered microorganisms”, Frontiers in Bioengineering and Biotechnology, vol. 6, 2018.
• A. Mishra, A. Malik, “Recent advances in microbial metal bioaccumulation”, Critical Reviews in Environmental Science and Technology, vol. 43, no. 11, pp. 1162-1222, 2013.
• N. D. Nnaji, H. Onyeaka, T. Miri, C. Ugwa, “Bioaccumulation for heavy metal removal: A review”, SN Applied Sciences, vol. 5, no. 125, 2023.
• M. Zabochnicka-Światek, M. Krzywonos, “Potentials of biosorption and bioaccumulation processes for heavy metal removal”, Polish Journal of Environmental Studies, vol. 23, no. 2, pp. 551-561, 2014.
• A. Y. Dursun, G. Uslu, O. Tepe, Y. Cuci, H. I. Ekiz, “A comparative investigation on the bioaccumulation of heavy metal ions by growing Rhizopus arrhizus and Aspergillus niger” Biochemical Engineering Journal, vol. 15, no. 2, pp. 87-92, 2003.
• F. Aslam, A. Yasmin, S. Sohail, “Bioaccumulation of lead, chromium, and nickel by bacteria from three different genera isolated from industrial effluent”, International Microbiology, vol. 23, no. 2, pp. 253-261, 2020.
• J. A. Mir-Tutusaus, R. Baccar, G. Caminal, M. Sarrà, “Can white-rot fungi be a real wastewater treatment alternative for organic micropollutants removal? A review”, Water Research, vol. 138, pp. 137-151, 2018.
• A. Singh, D. B. Pal, A. Mohammad, A. Alhazmi, S. Haque, T. Yoon, N. Srivastava, V. K. Gupta, “Biological remediation technologies for dyes and heavy metals in wastewater treatment: New insight”, Bioresource Technology, vol. 343, 2022.
• Y. Li, G. Zou, S. Yang, Z. Wang, T. Chen, X. Yu, Q. Guo, R. He, T. Duan, W. Zhu, “Integration of bio-inspired adsorption and photodegradation for the treatment of organics-containing radioactive wastewater”, Chemical Engineering Journal, vol. 364, pp. 139-145, 2019.
• Q. J. Choudhury, S. Ambati, Z. A. Lewis, R. B. Meagher, “Targeted delivery of antifungal liposomes to Rhizopus delemar”, Journal of Fungi, vol. 8, no. 4, 2022.
• O. A. Evirgen, Y. S. Acikel, “Simultaneous copper bioaccumulation, growth and lipase production of Rhizopus delemar in molasses medium: Optimisation of environmental conditions using RSM”, Chemistry and Ecology, vol. 30, no. 1, pp. 39-51, 2014.
• O. A. Evirgen, Y. S. Acikel, “Investigation of mutual interactions of physicochemical parameters on simultaneous Zn(II) bioaccumulation and lipase production of R. delemar”, Desalination and Water Treatment”, vol. 53, no. 13, pp. 3543-3556, 2015.
• F. Jamil Emon, M. F. Rohani, N. Sumaiya, M. F. Tuj Jannat, Y. Akter, M. Shahjahan, Z. Abdul Kari, A. B. Tahiluddin, K. W. Goh, “Bioaccumulation and bioremediation of heavy metals in fishes-A review”, Toxics, vol. 11, no. 6, 2023.
• A. Muhammed, A. Hussen, T. Kaneta, “Trace nickel analysis in water samples via paper-based devices coupled with co-precipitation”, South African Journal of Chemistry, vol. 77, pp. 01-07, 2023.
• D. F. Boltz, Colorimetric Methods of Analysis., Including Photometric Methods vol. 2A, Princeton: N.J, 1959.
• L. Rosso, J. R. Lobry, S. Bajard, J. P. Flandrois, “Convenient model to describe the combined effects of temperature and pH on microbial growth”, Applied and Environmental Microbiology, vol. 61, no. 2, pp. 610-616, 1995.
• J. M. Gonzalez, B. Aranda, “Microbial growth under limiting conditions-future perspectives”, Microorganisms, vol. 11, no.7, 2023.
• Ü. Açıkel, M. Erşan, “Investigation of inhibition kinetics of Zn(II) Ions on the acid phosphatase activity and growth of R. delemar and Zn(II) bioaccumulation”, Desalination and Water Treatment, vol. 57, no. 8, pp. 3689-3699, 2016.
• F. Huang, C.L. Guo, G. N. Lu, X. Y. Yi, L. D. Zhu, Z. Dang, “Bioaccumulation characterization of cadmium by growing Bacillus cereus RC-1 and its mechanism”, Chemosphere, vol. 109, pp. 134-142, 2014.
• A. Paul, T. R. Stösser, A. Zehl, E. Zwirnmann, R. D. Vogt, C. E. W. Steinberg, “Nature and abundance of organic radicals in natural organic matter: Effect of pH and irradiation”, Environmental Science & Technology, vol. 40, no. 19, pp. 5897-5903, 2006.
• A. Matin, “pH homeostasis in acidophiles”, Novartis Found Symp, vol. 221, pp. 152-166, 1999.
• C. M. Bethke, R. A. Sanford, M. F. Kirk, Q. Jin, T. M. Flynn, “The thermodynamic ladder in geomicrobiology”, American Journal of Science, vol. 311, no. 3, pp. 183-210, 2011.
• C. Abourached, T. Catal, H. Liu,” Efficacy of single-chamber microbial fuel cells for removal of cadmium and zinc with simultaneous electricity production”, Water Research, vol. 51, pp. 228-233, 2014.
• V. Kumar, S. K. Dwivedi, “Mycoremediation of heavy metals: processes, mechanisms, and affecting factors”, Environmental Science and Pollution Research, vol. 28, pp. 10375–10412, 2021.
• S. Zhang, H. Jiang, S. Xue, N. Ge, Y. Sun, Z. Chi, G. Liu, Z. Chi, “Efficient conversion of cane molasses into fructooligosaccharides by a glucose derepression mutant of aureobasidium melanogenum with high β-fructofuranosidase activity”, Journal of Agricultural and Food Chemistry, vol. 67, no. 49, pp. 13665-13672, 2019.
• S. Pattanakittivorakul, N. Lertwattanasakul, M. Yamada, S. Limtong, “Selection of thermotolerant Saccharomyces cerevisiae for high temperature ethanol production from molasses and increasing ethanol production by strain improvement”, International Journal of General and Molecular Microbiology, vol. 112, no. 7, pp. 975-990, 2019.
• G. Y. Yew, B. K. Puah, K. W. Chew, S. Y. Teng, P. L. Show, T. H. P. Nguyen, “Chlorella vulgaris FSP-E cultivation in waste molasses: Photo-to-property estimation by artificial intelligence”, Chemical Engineering Journal, vol. 402, 2020.
• G. Mersin, Ü. Açıkel, “Production of Candida biomasses for heavy metal removal from wastewaters”, Trakya University Journal of Natural Sciences, vol. 22, no. 1, pp. 67-76, 2020.
• X. Zi, Y. Liu, T. Chen, M. Li, H. Zhou, J. Tang, “Effects of sucrose, glucose and molasses on fermentation quality and bacterial community of stylo silage”, Fermentation, vol. 8, no. 5, 2022.
• Z. Aksu, G. Dönmez, “The use of molasses in copper(II) containing wastewaters: Effects on growth and copper(II) bioaccumulation properties of Kluyveromyces marxianus”, Process Biochemistry, vol. 36, no.5, pp. 451-458, 2000.
• Z. Aksu, G. Dönmez, “Combined effects of molasses sucrose and reactive dye on the growth and dye bioaccumulation properties of Candida tropicalis”, Process Biochemistry, vol. 40, no.7, pp. 2443-2454, 2005.
• W. R. Sadler, P. A. Trudinger, “The inhibition of microorganisms by heavy metals”, Mineralium Deposita, vol. 2, pp. 158-168, 1967.
• Ü. Açikel, T. Alp, “A study on the inhibition kinetics of bioaccumulation of Cu(II) and Ni(II) ions using Rhizopus delemar”, Journal of Hazardous Materials, vol. 168, no. 2–3, pp.1449-1458, 2009.
• A. Y. Dursun, G. Uslu, Y. Cuci, Z. Aksu, “Bioaccumulation of copper(II), lead(II) and chromium(VI) by growing Aspergillus niger”, Process Biochemistry, vol. 38, no. 12, pp. 1647-1651, 2003.
• Ü. Açikel, M. Erşan, “Acid phosphatase production by Rhizopus delemar: A role played in the Ni(II) bioaccumulation process”, Journal of Hazardous Materials, vol. 184, no. 1-3, pp. 632-639, 2010.
• A. Naskar, R. Majumder, M. Goswami, “Bioaccumulation of Ni(II) on growing cells of Bacillus sp.: Response surface modeling and mechanistic insight”, Environmental Technology & Innovation, vol. 20, 2020.
• M. Zhu, X. Feng, G. Qiu, J. Feng, L. Zhang, P. C. Brookes, J. Xu, Y. He, “Synchronous response in methanogenesis and anaerobic degradation of pentachlorophenol in flooded soil”, Journal of Hazardous Materials, vol. 374, pp. 258-266, 2019.
• T. M. Roane, I. L. Pepper, T. J. Gentry, Microorganisms and Metal Pollutants, in: Environmental Microbiology, 3rd. ed. Academic Press, Elsevier, 2015.
• T. R. Sandrin, R. M. Maier, “Impact of metals on the biodegradation of organic pollutants”, Environmental Health Perspectives, vol. 111, no. 8, pp. 1093- 1101, 2003.
• Q. Lu, X. Zou, J. Liu, Z. Liang, H. Shim, R. Qiu, S. Wang, “Inhibitory effects of metal ions on reductive dechlorination of polychlorinated biphenyls and perchloroethene in distinct organohalide-respiring bacteria”, Environment International, vol. 135, 2020.
• C. Li, W. Jiang, N. Ma, Y. Zhu, X. Dong, D. Wang, X. Meng, Y. Xu, “Bioaccumulation of cadmium by growing Zygosaccharomyces rouxii and Saccharomyces cerevisiae”, Bioresource Technology, vol. 155, pp.116-121, 2014.
• A. M. Stanescu, L. Stoica, C. Constantin, I. Lacatusu, O. Oprea, F. Miculescu, “Physicochemical characterization and use of heat pretreated commercial instant dry baker’s yeast as a potential biosorbent for Cu(II) removal”, Clean-Soil, Air, Water, vol. 42, no. 11, pp. 1632-1641, 2014.
• F. Mohamadhasani, M. Rahimi, “Growth response and mycoremediation of heavy metals by fungus Pleurotus sp.”, Scientific Reports, vol. 12, 2022.