Investigation of Inhibition Effect on Pathogen Microorganisms by Adding NPAg to Activated Carbon Obtained by Activating Hazelnut Shell with ZnCl2

Year 2025, Volume: 29 Issue: 3, 318 - 336, 26.06.2025

Birsen Sarıcı , Esra Altıntığ , Şükrü Karataş

https://doi.org/10.16984/saufenbilder.1659291

Abstract

In this study, silver-coated activated carbon (NPAgAC) was obtained by adding nanoparticle silver (NPAg) to activated carbon (AC) produced from hazelnut shells by chemical activation and carbonization. SEM, FT-IR, and BET were evaluated. While the methylene blue (MB) number of the produced ACs was 490-499 mg/g, the MB numbers of NPAgAC were determined to be 421-453 mg/g. The iodine number of ACs was specified in the 1047-1612 mg/g range. The iodine numbers of NPAgACs were recorded in the 934-1022 mg/ g range. Additionally, EDS and XRD analyses were performed on all samples. Well, diffusion and spreading plate methods were used to control the antimicrobial properties of the produced NPAgAC. The study used Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) as pathogenic microorganisms. In addition, the antimicrobial properties of NPAgACs were investigated using the spread plate method at various times and temperatures to determine their effectiveness in inhibiting the growth of E. coli in polluted waters. Studies have shown that hazelnut shells are a suitable starting material for producing activated carbon, and that NPAgAC exhibits high antimicrobial properties with food safety.

Keywords

Activated carbon, Food safety, Hazelnut shell, Antimicrobial, NPAgAC

References

• J. Baruah, B.K. Nath, R. Sharma, S. Kumar, R.C. Deka, D. C. Baruah, E. Kalita, “Recent Trends in the Pretreatment of Lignocellulosic Biomass for Value-Added Products,” Front Energy Res, vol. 6, no. 141, pp. 1-19, 2018.
• T. Q. Tuan, N. Van Son, H. T. K. Dung, H. L. Nguyen, T. T. Bui, T. V. A. Nguyen, D. H. Nguyen, H. H. Nguyen, “Preparation and properties of silver nanoparticles loaded in activated carbon for biological and environmental applications,” Journal Hazard Mater, vol. 192, no. 3, pp. 1321-1329, 2011.
• A. A. Mostafa, A. A. Al-Askar, K. S. Almaary, T. M. Dawoud, E. N. Sholkamy, M. M. Bakri, “Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases,” Saudi Journal of Biological Sciences, vol. 25, no. 2, pp. 361-366, 2018.
• A. Mani, M. Amalanathan, M. S. M. Mary, C. Parvathiraja, A. Alothman, S. M. Wabaidur, M. A. Islam, “Enhanced photocatalytic and biological observations of green synthesized activated carbon, activated carbon doped silver, and activated carbon/silver/titanium dioxide nanocomposites,” Journal of Inorganic Organometallic Polymers Materials, vol. 32, no. 1, pp. 267-279, 2022.
• W. Trisunaryanti, K. Wijaya, T. Riyono, N. Wahyuningtyas, S. P. Utami, L. Larasati, “Characteristics of coconut shell-based activated carbon as Ni and Pt catalyst support for hydrotreating Calophyllum inophyllum oil into hydrocarbon-based biofuel,” Journal of Environmental Chemical Engineering, vol. 10, no. 5, pp. 108-209, 2022.
• A. Sobhan, K. Muthukumarappan, L. Wei, T. Van Den Top, R. Zhou, “Development of an activated carbon-based nanocomposite film with antibacterial property for smart food packaging,” Materials Today Communications, vol. 23, pp. 101-m124, 2020.
• J. C. M. Márquez, A. H. Partida, M. del Carmen, M. Dosta, J. C. Mejía, J. A. B. “Martínez Silver nanoparticles applications (AgNPS) in aquaculture,” International Journal of Fisheries and Aquatic Studies, vol. 6, pp. 5-11, 2018.
• S. Yorgun, D. Yıldız, “Preparation and characterization of activated carbons from Paulownia wood by chemical activation with H3PO4,” Journal of the Taiwan Institute of Chemical Engineers, vol. 53, pp. 122-131, 2015.
• H. Teramura, E. Yasuda, Y. Naisei, “Impact of Spreading Time to Recovery Rate in Suitability Test of Solid Agar Media,” Biocontrol Science and Technology, vol. 26, no. 1, pp. 43-47, 2021.
• N. Söyler, J. L. Goldfarb, S. Ceylan, M. T. Saçan, “Renewable fuels from pyrolysis of Dunaliella tertiolecta: An alternative approach to biochemical conversions of microalgae,” Energy, vol. 120, pp. 907-914, 2017.
• H. Ç. Kazici, V. Yönten, M. R. Kivanç, M. Ertas, F. Salman, M. Yayla, “Antimicrobial properties of multimetallic of silver compounds immobilized on active carbon and nano carbon tubes,” Current Research in Green and Sustainable Chemistry, vol. 4, pp. 100-204, 2021.
• K. S. Ukanwa, K. Patchigolla, R. Sakrabani, E. Anthony, S. Mandavgane, “A review of chemicals to produce activated carbon from agricultural waste biomass,” Sustainability, vol. 11, no. 22, p. 6204, 2019.
• T. V. Duncan, “Applications of nanotechnology in food packaging and food safety: Barrier materials, antimicrobials, and sensors,” Journal of Colloid and Interface Science, vol. 363, no. 1, pp. 1-24, 2011.
• S. Moeinzadeh, E. Jabbari, “Nanoparticles and their applications. In Springer handbook of nanotechnology,” Springer, Berlin, Heidelberg, pp. 335-361, 2017.
• U. Latif, K. Al-Rubeaan, A. T. Saeb, “A review on antimicrobial chitosan-silver nanocomposites: A roadmap toward pathogen targeted synthesis,’’ International Journal of Polymeric Materials and Polymeric Biomaterials, vol. 64, no. 9, pp. 448-458, 2015.
• A. A. El-Sayed, A. M. Khalil, M. El-Shahat, N.Y. Khaireldin, S.T. Rabie, “Antimicrobial activity of PVC-pyrazolone-silver nanocomposites,’’ Journal of Macromolecular Science, Part A, vol. 53, no. 6, pp. 346-353, 2016.
• P. Chingombe, B. Saha, R. J. Wakeman, “Sorption of atrazine on conventional and surface modified activated carbons,” Journal of Colloid and Interface Science, vol. 302, no. 2, pp. 408-416, 2006.
• B. Sarıcı, Ş. Karatas, E. Altintig, “Removal of Methylene blue from aqueous solution with activated carbon produced from hazelnut shells by K2CO3 activation,” Desalination and Water Treatment, vol. 254, pp. 287-301, 2022.
• Y. Zhao, Z.O. Wang, X. Zhao, W. Li, S. X. Liu, “Antibacterial action of silver-doped activated carbon prepared by vacuum impregnation,” Applied Surface Science, vol. 266, pp. 67-72, 2013.
• S. Singh, A. Bharti, V. K. Meena, “Structural thermal zeta potential and electrical properties of disaccharide reduced silver nanoparticles,” Journal of Materials Science: Materials in Electronics, vol. 25, pp. 3747–52, 2014.
• C. G. Giske, J. Turnidge, R. Cantón, G. Kahlmeter, “Update from the European committee on antimicrobial susceptibility testing (EUCAST), Journal of Clinical Microbiology, vol. 60, no. 3, pp. e00276- 21, 2022.
• Y. Sudaryanto, S. B. Hartono, W. Irawaty, H. Hindarso, S. Ismadji, “High Surface area activated carbon prepared from cassava peel by chemical activation,” Bioresource Technology, vol. 97, pp. 734-739, 20076.
• M. Uyttendaele, E. Franz, O. Schlüter, “Food safety, a global challenge,” International Journal of Environmental Research and Public Health, vol. 13, no. 1, p. 67, 2016.
• P. Biswas, R. Bandyopadhyaya, “Water disinfection using silver nanoparticle-impregnated activated carbon: Escherichia coli cell-killing in batch and continuous packed column operation over a long duration,” Water Research, vol. 100, pp. 105-115, 2016.
• S. Thomas, R.A. Gonsalves, J. Jose, S. H. Zyoud, A. R. Prasad, J. Garvasis, “Plant-based synthesis, characterization approaches, applications and toxicity of silver nanoparticles: A comprehensive review,” Journal of Biotechnology, vol. 394, pp. 135-149, 2024.
• Y. Baran, H. S. Gökçe, M. Durmaz, “Physical and mechanical properties of cement containing regional hazelnut shell ash wastes,” Journal of Cleaner Production, vol. 259, p. 120965,2020.
• Ö. Bağ, K. Tekin K. “Atık lignoselülozik biyokütleden hidrotermal karbon üretimi ve karakterizasyonu,” Gazi Üniversitesi Mühendislik Mimarlık Fakültesi Dergisi, vol. 35 no. 2, pp. 1063-1076, 2019.
• X. Jian, X. Zhuang, B. Li, X. Xu, Z. Wei, Y. Song, E. Jiang, “Comparison of characterization and adsorption of biochars produced from hydrothermal carbonization and pyrolysis,” Environmental Technology & Innovation, vol. 10, pp. 27-35, 2018.
• Z. Heidarinejad, M. H. Dehghani, M. Heidari, G. Javedan, L. Ali, M. Sillanpää, “Methods for preparation and activation of activated carbon: A review,” Environmental Chemistry Letters, vol. 18, no. 2, pp. 393-415, 2020.
• Ç. Çuhadar, “Production and characterization of activated carbon from hazelnut shell and hazelnut husk,” (Master's thesis, Middle East Technical University), pp. 23-82, 2005.
• E. Altintig, B. Sarıcı, S. Karataş, “Prepared activated carbon from hazelnut shell where coated nanocomposite with Ag+ used for antibacterial and adsorption properties,” Environmental Science and Pollution Research, vol. 30, no. 5, pp. 13671-13687, 2023.
• D. Angın, E. Altintig, T. E. Köse, “Influence of process parameters on the surface and chemical properties of activated carbon obtained from biochar by chemical activation,” Bioresource Technology, vol. 148, pp. 542-549, 2013.
• C. Tang, Y. W. Sun, “Green and facile fabrication of silver nanoparticles loaded activated carbon fibers with long-lasting antibacterial activity,” RSC advances, vol. 4, no. 2, pp. 523-530, 2014.
• K. Y. Yoon, J. H. Byeon, C. W. Park, J. Hwang, “Antimicrobial effect of silver particles on bacterial contamination of activated carbon fibers,” Environmental Science & Technology, vol. 42, no. 4, pp. 1251-1255, 2008.
• T. C. Chandra, M. M. Mirna, Y. Sudaryanto, S. J. C. E. J. Ismadji, “Adsorption of basic dye onto activated carbon prepared from durian shell: Studies of adsorption equilibrium and kinetics,” Chemical Engineering Journal, vol. 127, no. 1-3, pp. 121-129, 2007.
• A. G. Blanco, J. A. de Oliveira, R. López, J. C. Moreno-Piraján, L. Giraldo, G. Zgrablich, K. Sapag, “A study of the pore size distribution for activated carbon monoliths and their relationship with the storage of methane and hydrogen. Colloids and Surfaces A,” Physicochemical and Engineering, vol. 357, no. 1-3, pp. 74-83, 2010.
• N. Korkmaz, A. Ceylan, Hamid, A. Karadağ, A. S. Bülbül, M. N. Aftab, O. Cevik, F. Sen, “Biogenic silver nanoparticles synthesized via Mimusops elengi fruit extract, a study on antibiofilm, antibacterial, and anticancer activities,” Journal of Drug Delivery Science and Technology, vol. 59, p. 101864, 2010.
• M. A. Islam, M. V. Jacob, E. Antunes, “A critical review on silver nanoparticles: From synthesis and applications to its mitigation through low-cost adsorption by biochar,” Journal of Environmental Management, vol. 28, p. 1111918, 2021.
• Ş. Karadirek, H. Okkay, “Ultrasound assisted green synthesis of silver nanoparticle attached activated carbon for levofloxacin adsorption,” Journal of the Taiwan Institute of Chemical Engineers, vol. 105, pp. 39-49, 2019.
• E. Altintig, S. Kirkil “Preparation and properties of Ag-coated activated carbon nanocomposites produced from wild chestnut shell by ZnCl2 activation,” Journal of Taiwan Institute of Chemical Engineers, vol. 63, pp. 180-188, 2016.
• S. Sharifan, “A comparative optimization study of activated carbon production from hazelnut shells by thermal and microwave heating methods,” (Doctoral dissertation, Imperial College London), pp. 212-231, 2014.
• E. Altintig, O. F. Soydan, “Methylene blue adsorption and preparation silver bound to activated carbon with sol-gel method,” Sakarya University Journal of Science, vol. 22, no. 6, pp. 1812-1819, 2018. L. Y. Le, F. Baron, M. Gautier, “Staphylococcus aureus and food poisoning,” Genetics and Molecular Research: GMR, vol. 2, no. 1, pp. 63-76, 2003.
• H. B. Haberecht, N. J. Nealon, J. R. Gilliland, A. V. Holder, C. Runyan, R. C. Oppel, E. P. Ryan, “Antimicrobial-resistant Escherichia coli from environmental waters in Northern Colorado,” Journal of Environmental and Public Health, vol. 2019, pp. 1-13, 2019.
• M. A. Lim, J. Y. Kim, D. Acharya, B. B. Bajgain, J. H. Park, S. J. Yoo, K. Lee, “A diarrhoeagenic enteropathogenic Escherichia coli (EPEC) infection outbreak that occurred among elementary school children in Gyeongsangbuk-Do province of South Korea was associated with consumption of water-contaminated food items,” International Journal of Environmental Research and Public Health, vol. 17, no. 9, pp. 1-19, 2020.
• A. Yurtay, M. Kılıc, “Fast and effective production of industrial grade activated carbon,” Journal of Porous Materials, vol. 30, pp. 1207-1220, 2023.
• S. Konyalı, M. Demir, “The agricultural policies applied for hazelnut and problems of hazelnut producers in Sakarya Province,” Eurasian Journal of Agricultural Economics, vol. 4, no. 1, pp. 11-21, 2024.
• M. A. Kara, “Investigation of hazelnut producing provinces with cluster analysis methods,” Ordu University Journal of Science and Technology, vol. 14, no. 2, pp. 396-410, 2024.