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Year 2011, Volume: 3 Issue: 1, 139 - 144, 14.08.2013

Abstract

References

  • Bandell M, Story GM, Hwang SW, Viswanath V, Eid SR, Petrus MJ, Earley TJ and Patapoutian A. 2004. Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin. Neuron 41: 849-857.
  • Bautista DM, Movahed P, Hinman A, Axelsson H E Sterner O, Högestätt ED, Julius D, Jordt SE and Zygmunt P. M. 2005. Pungent products from garlic activate the sensory ion channel TRPA1. Proc. Natl. Acad. Sci. U.S.A., 102: 12248-12252.
  • Bessac BF, Sivula M, von Hehn CA, Escalera J, Cohn L, Jordt SE. 2008. TRPA1 is a major oxidant sensor in murine airway sensory neurons. J Clin Invest 118: 1899-1910.
  • Corey DP, García-Añoveros J, Holt JR, Kwan KY, Lin SY, Vollrath MA, Amalfitano A, Cheung EL, Derfler BH, Duggan A, Géléoc GS, Gray PA, Hoffman MP, Rehm HL, Tamasauskas D, and Zhang DS. 2004. TRPA1 is a candidate for the mechanosensitive transduction channel of vertebrate hair cells. Nature 432: 723-730.
  • Duong Dinh TA, Jüngling E, Strotmann KH, Westhofen M, Lückhoff A. 2006. Ultrasonic bath depth control and regulation in single cell recordings. Pflugers Arch 452: 784-788.
  • Düwel P, Haasler T, Jüngling E, Duong TA, Westhofen M, Lückhoff A. 2005. Effects of cinnarizine on calcium and pressure-dependent potassium currents in guinea pig vestibular hair cells. Naunyn Schmiedebergs Arch Pharmacol 371: 441-448.
  • Fettiplace R. 2009. Defining features of the hair cell mechanoelectrical transducer channel. Pflügers Arch 458: 1115-1123.
  • Fischer MJ, Leffler A, Niedermirtl F, Kistner K, Eberhardt M, Reeh PW, Nau C. 2010. The general anesthetic propofol excites nociceptors by activating TRPV1 and TRPA1 rather than GABAA receptors. J Biol Chem 285: 3478134792.
  • Haasler T, Homann G, Duong Dinh TA, Jüngling E, Westhofen M, Lückhoff A. 2009.Pharmacological modulation of transmitter release by inhibition of pressure-dependent potassium currents in vestibular hair cells. Naunyn Schmiedebergs Arch Pharmacol 380: 531-538.
  • Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ. 1981. Improved patchclamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch 391: 85-100.
  • Hayashi K, Kobayashi R, Kitamura K, Goto F, Ogawa K, Matsumoto T. 2010. A novel model for prognosis of Meniere's disease using oxidative stress susceptibility of lymphoblastoid cell lines. Biosci Trends 4: 72-78.
  • Hill K, Schaefer M. 2008. Ultraviolet light and photosensitising agents activate TRPA1 via generation of oxidative stress. Cell Calcium 45: 155-164.
  • Jordt SE, Bautista DM, Chuang HH, McKemy DD, Zygmunt PM, Högestätt ED, Meng ID and Julius D. 2004. Mustard oils and cannabinoids excite sensory nerve fibres through the TRP channel ANKTM1. Nature 427: 260-265.
  • Kim D, Cavanaugh EJ, Simkin D. 2008. Inhibition of transient receptor potential A1 channel by phosphatidylinositol-4,5-bisphosphate. Am J Physiol Cell Physiol 295: C92-99.
  • Knox GW, McPherson A. 1997. Menière's disease: differential diagnosis and treatment. Am Fam Physician 55: 1185-1190, 1193-1194.
  • Kwan KY, Allchorne AJ, Vollrath MA, Christensen AP, Zhang DS, Woolf CJ and Corey DP. 2006. TRPA1 contributes to cold, mechanical, and chemical nociception but is not essential for hair-cell transduction. Neuron 50: 177180.
  • Labbé D, Teranishi MA, Hess A, Bloch W, Michel O. 2005. Activation of caspase-3 is associated with oxidative stress in the hydropic guinea pig cochlea. Hear Res 202: 21-27.
  • Lee SP, Buber MT, Yang Q, Cerne R, Cortés RY, Sprous DG, Bryant RW. Br J Pharmacol. 2008. Thymol and related alkyl phenols activate the hTRPA1 channel. 153: 1739-1749.
  • Lin CY, Young YH. 2001. Effect of smoking on the treatment of vertigo. Otol Neurotol 22: 369-372.
  • Macpherson LJ, Geierstanger BH, Viswanath V, Bandell M, Eid SR, Hwang S, Patapoutian A. 2005. The pungency of garlic: activation of TRPA1 and TRPV1 in response to allicin. Curr Biol 15: 929-934.
  • Macpherson LJ, Xiao B, Kwan KY, Petrus MJ, Dubin AE, Hwang S, Cravatt B, Corey DP and Patapoutian A. 2007. An ion channel essential for sensing chemical damage. J Neurosci 17;27: 11412-11415.
  • Nagata K, Duggan A, Kumar G. and García-Añoveros J. 2005. Nociceptor and hair cell transducer properties of TRPA1, a channel for pain and hearing. J Neurosci 25: 4052-4061.
  • Niforatos W, Zhang XF, Lake MR, Walter KA, Neelands T, Holzman TF, Scott VE, Faltynek CR, Moreland RB and Chen J. 2007. Activation of TRPA1 channels by the fatty acid amide hydrolase inhibitor 3’-carbamoylbiphenyl-3-yl cyclohexylcarbamate (URB597). Mol. Pharmacol 71: 1209-1216.
  • Patil MJ, Jeske NA, Akopian AN. 2010. Transient receptor potential V1 regulates activation and modulation of transient receptor potential A1 by Ca2+. Neuroscience 171: 1109-1119.
  • Ricci AJ, Cochran SL, Rennie KJ, Correia MJ. 1997. Vestibular type I and type II hair cells. 2: Morphometric comparisons of dissociated pigeon hair cells. J Vestib Res 7: 407-420.
  • Takumida M, Ishibashi T, Hamamoto T, Hirakawa K, and Anniko, M. 2008. Expression of transient receptor potential channel melastin (TRPM) 1-8 and TRPA1 (ankyrin) in mouse inner ear. Acta Otolaryngol. 1-11.
  • Taylor-Clark TE, McAlexander MA, Nassenstein C, Sheardown SA, Wilson S, Thornton J, Carr MJ, Undem BJ. 2008. Relative contributions of TRPA1 and TRPV1 channels in the activation of vagal bronchopulmonary C-fibres by the endogenous autacoid 4-oxononenal. J Physiol 586: 3447-3459.
  • Talavera K, Gees M, Karashima Y, Meseguer VM, Vanoirbeek JA, Damann N, Everaerts W, Benoit M, Janssens A, Vennekens R, Viana F, Nemery B, Nilius B, Voets T. 2009. Nicotine activates the chemosensory cation channel TRPA1. Nat. Neurosci 12: 1293-1299.
  • Vilceanu D, Stucky CL. 2010. TRPA1 mediates mechanical currents in the plasma membrane of mouse sensory neurons. PLoS One 5: 12177.
  • Zurborg S, Yurgionas B, Jira JA, Caspani O, Heppenstall PA. 2007. Direct activation of the ion channel TRPA1 by Ca2+. Nat. Neurosci 10: 277-279.

Functional expression of TRPA1 cation channels in vestibular type II hair cells

Year 2011, Volume: 3 Issue: 1, 139 - 144, 14.08.2013

Abstract

Although the expression of TRPA1 proteins has been demonstrated in hair cells of the inner ear, the role of this Ca2+-permeable cation channel is unclear because TRPA1 knock-out mice have normal transduction currents in hair cells and do not show hearing impairment or vestibular problems. To test whether TRPA1 expression leads to the formation of intact ion channels in the plasma membrane of vestibular type II hair cells in the guinea pig, we measured whole-cell currents before and after stimulation with the specific agonists of TRPA1, allylisothiocyanate (AITC, 200 – 400 µM) and 3'-carbamoylbiphenyl-3-yl cyclohexylcarbamate (URB597, 100 µM). AITC induced currents with the typical current-voltage relation of TRPA1, as found in heterologous expression models. Currents densities reached maxima 168 ± 22 pA/pF (n = 41) at a holding potential of +60 mV and -62 ± 16 pA/pF at -60 mV. Current kinetics were characterized by an initial increase in amplitude over about 60 s, a subsequent plateau, and a complete current decline after wash-out of the drug. Repeated stimulations were possible. In the presence of URB597, similar currents developed but showed rapid desensitization under ongoing stimulation. We conclude that there is functional expression of TRPA1 in vestibular hair cells, at a current density relatively small in comparison to voltage gated currents. Thus, TRPA1 currents may modulate the electrical responses of hair cells. This may be relevant as potential side effects of many drugs and substances known to be activators of the polymodal channels TRPA1.

References

  • Bandell M, Story GM, Hwang SW, Viswanath V, Eid SR, Petrus MJ, Earley TJ and Patapoutian A. 2004. Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin. Neuron 41: 849-857.
  • Bautista DM, Movahed P, Hinman A, Axelsson H E Sterner O, Högestätt ED, Julius D, Jordt SE and Zygmunt P. M. 2005. Pungent products from garlic activate the sensory ion channel TRPA1. Proc. Natl. Acad. Sci. U.S.A., 102: 12248-12252.
  • Bessac BF, Sivula M, von Hehn CA, Escalera J, Cohn L, Jordt SE. 2008. TRPA1 is a major oxidant sensor in murine airway sensory neurons. J Clin Invest 118: 1899-1910.
  • Corey DP, García-Añoveros J, Holt JR, Kwan KY, Lin SY, Vollrath MA, Amalfitano A, Cheung EL, Derfler BH, Duggan A, Géléoc GS, Gray PA, Hoffman MP, Rehm HL, Tamasauskas D, and Zhang DS. 2004. TRPA1 is a candidate for the mechanosensitive transduction channel of vertebrate hair cells. Nature 432: 723-730.
  • Duong Dinh TA, Jüngling E, Strotmann KH, Westhofen M, Lückhoff A. 2006. Ultrasonic bath depth control and regulation in single cell recordings. Pflugers Arch 452: 784-788.
  • Düwel P, Haasler T, Jüngling E, Duong TA, Westhofen M, Lückhoff A. 2005. Effects of cinnarizine on calcium and pressure-dependent potassium currents in guinea pig vestibular hair cells. Naunyn Schmiedebergs Arch Pharmacol 371: 441-448.
  • Fettiplace R. 2009. Defining features of the hair cell mechanoelectrical transducer channel. Pflügers Arch 458: 1115-1123.
  • Fischer MJ, Leffler A, Niedermirtl F, Kistner K, Eberhardt M, Reeh PW, Nau C. 2010. The general anesthetic propofol excites nociceptors by activating TRPV1 and TRPA1 rather than GABAA receptors. J Biol Chem 285: 3478134792.
  • Haasler T, Homann G, Duong Dinh TA, Jüngling E, Westhofen M, Lückhoff A. 2009.Pharmacological modulation of transmitter release by inhibition of pressure-dependent potassium currents in vestibular hair cells. Naunyn Schmiedebergs Arch Pharmacol 380: 531-538.
  • Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ. 1981. Improved patchclamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch 391: 85-100.
  • Hayashi K, Kobayashi R, Kitamura K, Goto F, Ogawa K, Matsumoto T. 2010. A novel model for prognosis of Meniere's disease using oxidative stress susceptibility of lymphoblastoid cell lines. Biosci Trends 4: 72-78.
  • Hill K, Schaefer M. 2008. Ultraviolet light and photosensitising agents activate TRPA1 via generation of oxidative stress. Cell Calcium 45: 155-164.
  • Jordt SE, Bautista DM, Chuang HH, McKemy DD, Zygmunt PM, Högestätt ED, Meng ID and Julius D. 2004. Mustard oils and cannabinoids excite sensory nerve fibres through the TRP channel ANKTM1. Nature 427: 260-265.
  • Kim D, Cavanaugh EJ, Simkin D. 2008. Inhibition of transient receptor potential A1 channel by phosphatidylinositol-4,5-bisphosphate. Am J Physiol Cell Physiol 295: C92-99.
  • Knox GW, McPherson A. 1997. Menière's disease: differential diagnosis and treatment. Am Fam Physician 55: 1185-1190, 1193-1194.
  • Kwan KY, Allchorne AJ, Vollrath MA, Christensen AP, Zhang DS, Woolf CJ and Corey DP. 2006. TRPA1 contributes to cold, mechanical, and chemical nociception but is not essential for hair-cell transduction. Neuron 50: 177180.
  • Labbé D, Teranishi MA, Hess A, Bloch W, Michel O. 2005. Activation of caspase-3 is associated with oxidative stress in the hydropic guinea pig cochlea. Hear Res 202: 21-27.
  • Lee SP, Buber MT, Yang Q, Cerne R, Cortés RY, Sprous DG, Bryant RW. Br J Pharmacol. 2008. Thymol and related alkyl phenols activate the hTRPA1 channel. 153: 1739-1749.
  • Lin CY, Young YH. 2001. Effect of smoking on the treatment of vertigo. Otol Neurotol 22: 369-372.
  • Macpherson LJ, Geierstanger BH, Viswanath V, Bandell M, Eid SR, Hwang S, Patapoutian A. 2005. The pungency of garlic: activation of TRPA1 and TRPV1 in response to allicin. Curr Biol 15: 929-934.
  • Macpherson LJ, Xiao B, Kwan KY, Petrus MJ, Dubin AE, Hwang S, Cravatt B, Corey DP and Patapoutian A. 2007. An ion channel essential for sensing chemical damage. J Neurosci 17;27: 11412-11415.
  • Nagata K, Duggan A, Kumar G. and García-Añoveros J. 2005. Nociceptor and hair cell transducer properties of TRPA1, a channel for pain and hearing. J Neurosci 25: 4052-4061.
  • Niforatos W, Zhang XF, Lake MR, Walter KA, Neelands T, Holzman TF, Scott VE, Faltynek CR, Moreland RB and Chen J. 2007. Activation of TRPA1 channels by the fatty acid amide hydrolase inhibitor 3’-carbamoylbiphenyl-3-yl cyclohexylcarbamate (URB597). Mol. Pharmacol 71: 1209-1216.
  • Patil MJ, Jeske NA, Akopian AN. 2010. Transient receptor potential V1 regulates activation and modulation of transient receptor potential A1 by Ca2+. Neuroscience 171: 1109-1119.
  • Ricci AJ, Cochran SL, Rennie KJ, Correia MJ. 1997. Vestibular type I and type II hair cells. 2: Morphometric comparisons of dissociated pigeon hair cells. J Vestib Res 7: 407-420.
  • Takumida M, Ishibashi T, Hamamoto T, Hirakawa K, and Anniko, M. 2008. Expression of transient receptor potential channel melastin (TRPM) 1-8 and TRPA1 (ankyrin) in mouse inner ear. Acta Otolaryngol. 1-11.
  • Taylor-Clark TE, McAlexander MA, Nassenstein C, Sheardown SA, Wilson S, Thornton J, Carr MJ, Undem BJ. 2008. Relative contributions of TRPA1 and TRPV1 channels in the activation of vagal bronchopulmonary C-fibres by the endogenous autacoid 4-oxononenal. J Physiol 586: 3447-3459.
  • Talavera K, Gees M, Karashima Y, Meseguer VM, Vanoirbeek JA, Damann N, Everaerts W, Benoit M, Janssens A, Vennekens R, Viana F, Nemery B, Nilius B, Voets T. 2009. Nicotine activates the chemosensory cation channel TRPA1. Nat. Neurosci 12: 1293-1299.
  • Vilceanu D, Stucky CL. 2010. TRPA1 mediates mechanical currents in the plasma membrane of mouse sensory neurons. PLoS One 5: 12177.
  • Zurborg S, Yurgionas B, Jira JA, Caspani O, Heppenstall PA. 2007. Direct activation of the ion channel TRPA1 by Ca2+. Nat. Neurosci 10: 277-279.
There are 30 citations in total.

Details

Primary Language English
Journal Section Original Articles
Authors

İngo Sparrer This is me

Thien An Duong Dinh This is me

Eberhard Jüngling This is me

Martin Westhofen This is me

Andreas Lückhoff

Publication Date August 14, 2013
Published in Issue Year 2011 Volume: 3 Issue: 1

Cite

APA Sparrer, İ., Duong Dinh, T. A., Jüngling, E., Westhofen, M., et al. (2013). Functional expression of TRPA1 cation channels in vestibular type II hair cells. Cell Membranes and Free Radical Research, 3(1), 139-144.
AMA Sparrer İ, Duong Dinh TA, Jüngling E, Westhofen M, Lückhoff A. Functional expression of TRPA1 cation channels in vestibular type II hair cells. Cell Membranes and Free Radical Research. August 2013;3(1):139-144.
Chicago Sparrer, İngo, Thien An Duong Dinh, Eberhard Jüngling, Martin Westhofen, and Andreas Lückhoff. “Functional Expression of TRPA1 Cation Channels in Vestibular Type II Hair Cells”. Cell Membranes and Free Radical Research 3, no. 1 (August 2013): 139-44.
EndNote Sparrer İ, Duong Dinh TA, Jüngling E, Westhofen M, Lückhoff A (August 1, 2013) Functional expression of TRPA1 cation channels in vestibular type II hair cells. Cell Membranes and Free Radical Research 3 1 139–144.
IEEE İ. Sparrer, T. A. Duong Dinh, E. Jüngling, M. Westhofen, and A. Lückhoff, “Functional expression of TRPA1 cation channels in vestibular type II hair cells”, Cell Membranes and Free Radical Research, vol. 3, no. 1, pp. 139–144, 2013.
ISNAD Sparrer, İngo et al. “Functional Expression of TRPA1 Cation Channels in Vestibular Type II Hair Cells”. Cell Membranes and Free Radical Research 3/1 (August 2013), 139-144.
JAMA Sparrer İ, Duong Dinh TA, Jüngling E, Westhofen M, Lückhoff A. Functional expression of TRPA1 cation channels in vestibular type II hair cells. Cell Membranes and Free Radical Research. 2013;3:139–144.
MLA Sparrer, İngo et al. “Functional Expression of TRPA1 Cation Channels in Vestibular Type II Hair Cells”. Cell Membranes and Free Radical Research, vol. 3, no. 1, 2013, pp. 139-44.
Vancouver Sparrer İ, Duong Dinh TA, Jüngling E, Westhofen M, Lückhoff A. Functional expression of TRPA1 cation channels in vestibular type II hair cells. Cell Membranes and Free Radical Research. 2013;3(1):139-44.