ISPARTA BÖLGESİNDEKİ GÜL YAĞININ KİMYASAL İÇERİĞİNİN GC-MS VE FTIR SPEKTROSKOPİSİ TEKNİĞİ İLE İNCELENMESİ

Abstract

Özet: Bu çalışmada, Isparta'dan toplanan gül yapraklarından özütlenen örnekler GC-MS cihazında kimyasal kompozisyonu belirlendi. Titreşim Spektroskopisi için FTIR Spektrometresi kullanılarak gül yağına ait karakteristik frekanslar belirlenmiştir. 3332 cm-1 de görülen band gül yağı bileşenlerinden fenole ait olan hidroksil grubudur. Gül yağı bileşenlerine ait diğer alt gruplar metil ve metilen grupları başat olan frekans bandları olarak kırmızı altı spektrumda gözlendi. Anahtar Kelimeler: Gül, FTIR, GC-MS INVESTIGATION OF CHEMICAL COMPOSITION OF ROSE OIL FROM ISPARTA REGION BY GC-MS AND FTIR SPECTROSCOPY TECHNIQUE Abstract: In this study, collected leaves of roses from Isparta were extracted and then Chemical composition of rose oil obtained by GC-MS. Vibrational spectroscopy is used to identify characteristic frequencies of rose oil by FTIR Spectrometer. The band at 3322 cm-1 assigned to hydroxyl group which belongs to phenol compounds of essential rose oil and methyl and methylene groups which are other functional groups of components in essential rose oil are observed as dominant frequencies by IR spectrometer. Keywords: Rose, FTIR, GC-MS

Keywords

• Gül,FTIR,GC-MS

ISPARTA BÖLGESİNDEKİ GÜL YAĞININ KİMYASAL İÇERİĞİNİN GC-MS VE FTIR SPEKTROSKOPİSİ TEKNİĞİ İLE İNCELENMESİ

Abstract

Özet: Bu çalışmada, Isparta'dan toplanan gül yapraklarından özütlenen örnekler GC-MS cihazında kimyasal kompozisyonu belirlendi. Titreşim Spektroskopisi için FTIR Spektrometresi kullanılarak gül yağına ait karakteristik frekanslar belirlenmiştir. 3332 cm-1 de görülen band gül yağı bileşenlerinden fenole ait olan hidroksil grubudur. Gül yağı bileşenlerine ait diğer alt gruplar metil ve metilen grupları başat olan frekans bandları olarak kırmızı altı spektrumda gözlendi. Anahtar Kelimeler: Gül, FTIR, GC-MS INVESTIGATION OF CHEMICAL COMPOSITION OF ROSE OIL FROM ISPARTA REGION BY GC-MS AND FTIR SPECTROSCOPY TECHNIQUE Abstract: In this study, collected leaves of roses from Isparta were extracted and then Chemical composition of rose oil obtained by GC-MS. Vibrational spectroscopy is used to identify characteristic frequencies of rose oil by FTIR Spectrometer. The band at 3322 cm-1 assigned to hydroxyl group which belongs to phenol compounds of essential rose oil and methyl and methylene groups which are other functional groups of components in essential rose oil are observed as dominant frequencies by IR spectrometer. Keywords: Rose, FTIR, GC-MS

Keywords

• Gül,FTIR,GC-MS

References

1. AĞAOGLU YS, 2000. Rose oil industry and the production of oil rose (Rosa damascena Mill.) in Turkey, Biotechnology & Biotechnological Equipment ,14 (2), 8-15
2. BAYDAR H, BAYDAR NG, 2005. The effects of harvest date, fermentation duration and Tween 20 treatment on essential oil content and composition of industrial oil rose (Rosa damascena Mill.), Industrial Crops and Products, 21, 251–255.
3. BAYRAK A, AKGÜL A, 1994. Volatile Oil Composition of Turkish Rose (Rosa damascena). J. Sci. Food Agric. 64, 441-448
4. COLTHUP NB, DALY LH, WIBERLEY SE, 1964. Introduction to Infrared and Raman Spectroscopy, Academic Press, London. EIKANI MH, GOLMOHAMMAD F, ROWSHANZAMIR S, MIRZA M, 2005. Recovery of water-soluble constituents of rose oil using simultaneous distillation–extraction, Flavour Fragr. J., 20, 555–558.
5. ERCİSLİ S, 2005. Rose (Rosa spp.) germplasm resources of Turkey, Genetic Resources and Crop Evolution) 52, 787–795.
6. JIROVETZ L, BUCHBAUER, STOYANOVA GA, BALINOVA A, GUANGJIUN Z, XIHAN M, 2005. Solid phase microextraction/gas chromatographic and olfactory analysis of the scent and fixative properties of the essential oil of Rosa damascena L. from China, Flavour Fragr. J. 20, 7–12.
7. KAMEI J, SAITOH A, KARITA T, 2005. Anxiolytic- and antistress-like effects of rose oil contained aroma tape in the elevated plus maze test and the holeboard test in mice, Meeting Abstract of Journal Of Pharmacological Sciences, 97, 160P-160P Suppl.
8. ÖZEL MZ, GÖĞÜS F, LEWIS AC, 2006. Comparison of direct thermal desorption with water distillation and superheated water extraction for the analysis of volatile components of Rosa damascena Mill. using GCxGC-TOF/MS, Analytica Chimica Acta, 566, 172–177.

9. RUSANOV K, KOVACHEVA N, VOSMAN B, ZHANG L, RAJAPAKSE S, ATANASSOV A, ATANASSOV I, 2005. Microsatellite analysis of Rosa damascena Mill. accessions reveals genetic similarity between genotypes used for rose oil production and old Damask rose varieties, Theor Appl Genet, 111, 804–809.
10. SILVERSTEIN RM, BASSLER GC, MORRILL TC, 1991. Spectrometric Identification of Organic Compounds, John Wiley and Sons. Inc., New York, SMITH B, 1999. Infrared Spectral Interpretation, A Systematic Approach, CRC Press, New York.
11. TUCKER AO, MACIARELLO M, 1988. A World Perspective, Flavors and Fragrances, Elsevier, Amsterdam
12. ZARGHAMI M, FARIN D, KHALAJ L, 2005, Evaluation of antidepressant activities of rose oil and geranium oil in the mouse forced-swim test, Meeting Abstract of Behavioural Pharmacology, 16, S42-S42 Suppl.