GLUKOZ 6-FOSFAT DEHIDROGENAZ ÜZERİNE BAZI SİTOTOKSİK KİMYASALLARIN ETKİSİ

İsmail Özmen

doi:10.29233/sdufeffd.134671

GLUKOZ 6-FOSFAT DEHIDROGENAZ ÜZERİNE BAZI SİTOTOKSİK KİMYASALLARIN ETKİSİ

Abstract

Özet: Bu çalışmanın amacı, glukoz 6-fosfat dehidrogenaz (G6PD) üzerine bazı sitotoksik kimyasalların in vitro etkilerini araştırmaktır. Başlangıçta, G6PD enzimi insan eritrositlerinden amonyum sülfat çöktürmesi ile kısmi olarak ve bunu takiben afinite kromatografisi ile saflaştırıldı. Inhibisyon etkisi gösteren ilaçların I50 değerleri %aktivite - ilaç konsantrasyonu grafiği ile belirlendi. Saflaştırılan enzim üzerinde araştırılan iki ilacın (2,4-dihidroksi-5-floropirimidin ve sodyum 2-sulfaniletansulfonat) enzimi inhibe ettiği belirlendi. Anahtar kelimeler: Glukoz 6-fosfat dehidrogenaz, afinite kromatografisi, saflaştırma, enzim inhibisyonu EFFECT OF SOME CYTOTOXIC CHEMICALS ON GLUCOSE 6-PHOSPHATE DEHYDROGENASE Abstract: The objective of this study was to determinate in vitro effects of some cytotoxic chemicals (2,4-dihydroxide-5-fluoropirimidine and sodium 2-sulfanylethanesulfonate) on glucose–6-phosphate dehydrogenase (G6PD). Initially, G6PD was purified from human erythrocyte by using ammonium sulphate precipitation and affinity chromatography. I50 values of the drugs exhibiting inhibition effects were determined by means of [drug]-activity% graph. The drugs investigated on the purified enzyme inhibited the enzyme activity. Key words: Glucose-6-phosphate dehydrogenase, affinity chromatography, purification, enzyme inhibition

Keywords

Glukoz 6-fosfat dehidrogenaz, afinite kromatografisi, saflaştırma, enzim inhibisyonu

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Details

Primary Language

Turkish

Subjects

-

Journal Section

-

Authors

İsmail Özmen This is me

Publication Date

June 8, 2009

Submission Date

June 8, 2009

Acceptance Date

-

Published in Issue

Year 2009 Volume: 4 Number: 1

DOI

https://doi.org/10.29233/sdufeffd.134671

IZ

https://izlik.org/JA68JG87BX

Cite

RIS / Bibtex

APA

Özmen, İ. (2009). GLUKOZ 6-FOSFAT DEHIDROGENAZ ÜZERİNE BAZI SİTOTOKSİK KİMYASALLARIN ETKİSİ. Süleyman Demirel University Faculty of Arts and Science Journal of Science, 4(1), 112-119. https://doi.org/10.29233/sdufeffd.134671

AMA

1.Özmen İ. GLUKOZ 6-FOSFAT DEHIDROGENAZ ÜZERİNE BAZI SİTOTOKSİK KİMYASALLARIN ETKİSİ. Süleyman Demirel University Faculty of Arts and Science Journal of Science. 2009;4(1):112-119. doi:10.29233/sdufeffd.134671

Chicago

Özmen, İsmail. 2009. “GLUKOZ 6-FOSFAT DEHIDROGENAZ ÜZERİNE BAZI SİTOTOKSİK KİMYASALLARIN ETKİSİ”. Süleyman Demirel University Faculty of Arts and Science Journal of Science 4 (1): 112-19. https://doi.org/10.29233/sdufeffd.134671.

EndNote

Özmen İ (June 1, 2009) GLUKOZ 6-FOSFAT DEHIDROGENAZ ÜZERİNE BAZI SİTOTOKSİK KİMYASALLARIN ETKİSİ. Süleyman Demirel University Faculty of Arts and Science Journal of Science 4 1 112–119.

IEEE

[1]İ. Özmen, “GLUKOZ 6-FOSFAT DEHIDROGENAZ ÜZERİNE BAZI SİTOTOKSİK KİMYASALLARIN ETKİSİ”, Süleyman Demirel University Faculty of Arts and Science Journal of Science, vol. 4, no. 1, pp. 112–119, June 2009, doi: 10.29233/sdufeffd.134671.

ISNAD

Özmen, İsmail. “GLUKOZ 6-FOSFAT DEHIDROGENAZ ÜZERİNE BAZI SİTOTOKSİK KİMYASALLARIN ETKİSİ”. Süleyman Demirel University Faculty of Arts and Science Journal of Science 4/1 (June 1, 2009): 112-119. https://doi.org/10.29233/sdufeffd.134671.

JAMA

1.Özmen İ. GLUKOZ 6-FOSFAT DEHIDROGENAZ ÜZERİNE BAZI SİTOTOKSİK KİMYASALLARIN ETKİSİ. Süleyman Demirel University Faculty of Arts and Science Journal of Science. 2009;4:112–119.

MLA

Özmen, İsmail. “GLUKOZ 6-FOSFAT DEHIDROGENAZ ÜZERİNE BAZI SİTOTOKSİK KİMYASALLARIN ETKİSİ”. Süleyman Demirel University Faculty of Arts and Science Journal of Science, vol. 4, no. 1, June 2009, pp. 112-9, doi:10.29233/sdufeffd.134671.

Vancouver

1.İsmail Özmen. GLUKOZ 6-FOSFAT DEHIDROGENAZ ÜZERİNE BAZI SİTOTOKSİK KİMYASALLARIN ETKİSİ. Süleyman Demirel University Faculty of Arts and Science Journal of Science. 2009 Jun. 1;4(1):112-9. doi:10.29233/sdufeffd.134671