NAA-BAP Effects on Callus Induction and Morphology in Gypsophila arrostii

Cahit Burak Küçükiğci; Rağbet Ezgi Duran

doi:10.29233/sdufeffd.1901262

NAA-BAP Effects on Callus Induction and Morphology in Gypsophila arrostii

Abstract

Plant tissue culture is a key tool for propagation, biotechnological production, and ex situ conservation because dedifferentiation can be directed under controlled conditions. Here, we quantitatively assessed the effects of 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) combinations on callus induction and morphological outputs (color and size) in Gypsophila arrostii leaf explants. Callus formation was obtained in 208 out of 273 explants, with induction rates ranging from 65.57% to 93.33% across media. The highest induction occurred on 2.0 mg/L NAA + 1.0 mg/L BAP (93.33%) and 2.0 mg/L NAA + 0.5 mg/L BAP (90.38%). Callus color composition varied among treatments: a pronounced yellow-dominant profile emerged under 1.5 mg/L NAA + 0.5 mg/L BAP, whereas the remaining treatments generally showed white-dominant or near white–yellow distributions. Most calli fell within the 0.3-0.6 cm size class, and size-class distributions did not differ significantly among hormone combinations. Overall, our findings indicate that auxin-cytokinin balance is likely a major determinant of callus induction performance in G. arrostii, providing a data-driven baseline for protocol optimization and subsequent regeneration-oriented studies.

Keywords

Supporting Institution

Süleyman Demirel University Scientific Research Projects Coordination

Project Number

FDK-2023-9195

Thanks

This study is part of the PhD thesis of Cahit Burak Küçükiğci conducted in the Plant Biotechnology Laboratory, Department of Biology, Süleyman Demirel University. The authors gratefully acknowledge the financial support provided by the Scientific Research Projects Coordination Unit of Süleyman Demirel University.

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Details

Primary Language

English

Subjects

Plant Biochemistry

Journal Section

Research Article

Authors

Cahit Burak Küçükiğci ^*
0000-0001-9317-7013
Türkiye

Rağbet Ezgi Duran
0000-0003-0853-3680
Türkiye

Publication Date

May 28, 2026

Submission Date

March 2, 2026

Acceptance Date

April 17, 2026

Published in Issue

Year 2026 Volume: 21 Number: 1

DOI

https://doi.org/10.29233/sdufeffd.1901262

IZ

https://izlik.org/JA74KK64JW

Cite

RIS / Bibtex

APA

Küçükiğci, C. B., & Duran, R. E. (2026). NAA-BAP Effects on Callus Induction and Morphology in Gypsophila arrostii. Süleyman Demirel University Faculty of Arts and Science Journal of Science, 21(1), 78-91. https://doi.org/10.29233/sdufeffd.1901262

AMA

1.Küçükiğci CB, Duran RE. NAA-BAP Effects on Callus Induction and Morphology in Gypsophila arrostii. Süleyman Demirel University Faculty of Arts and Science Journal of Science. 2026;21(1):78-91. doi:10.29233/sdufeffd.1901262

Chicago

Küçükiğci, Cahit Burak, and Rağbet Ezgi Duran. 2026. “NAA-BAP Effects on Callus Induction and Morphology in Gypsophila Arrostii”. Süleyman Demirel University Faculty of Arts and Science Journal of Science 21 (1): 78-91. https://doi.org/10.29233/sdufeffd.1901262.

EndNote

Küçükiğci CB, Duran RE (May 1, 2026) NAA-BAP Effects on Callus Induction and Morphology in Gypsophila arrostii. Süleyman Demirel University Faculty of Arts and Science Journal of Science 21 1 78–91.

IEEE

[1]C. B. Küçükiğci and R. E. Duran, “NAA-BAP Effects on Callus Induction and Morphology in Gypsophila arrostii”, Süleyman Demirel University Faculty of Arts and Science Journal of Science, vol. 21, no. 1, pp. 78–91, May 2026, doi: 10.29233/sdufeffd.1901262.

ISNAD

Küçükiğci, Cahit Burak - Duran, Rağbet Ezgi. “NAA-BAP Effects on Callus Induction and Morphology in Gypsophila Arrostii”. Süleyman Demirel University Faculty of Arts and Science Journal of Science 21/1 (May 1, 2026): 78-91. https://doi.org/10.29233/sdufeffd.1901262.

JAMA

1.Küçükiğci CB, Duran RE. NAA-BAP Effects on Callus Induction and Morphology in Gypsophila arrostii. Süleyman Demirel University Faculty of Arts and Science Journal of Science. 2026;21:78–91.

MLA

Küçükiğci, Cahit Burak, and Rağbet Ezgi Duran. “NAA-BAP Effects on Callus Induction and Morphology in Gypsophila Arrostii”. Süleyman Demirel University Faculty of Arts and Science Journal of Science, vol. 21, no. 1, May 2026, pp. 78-91, doi:10.29233/sdufeffd.1901262.

Vancouver

1.Cahit Burak Küçükiğci, Rağbet Ezgi Duran. NAA-BAP Effects on Callus Induction and Morphology in Gypsophila arrostii. Süleyman Demirel University Faculty of Arts and Science Journal of Science. 2026 May 1;21(1):78-91. doi:10.29233/sdufeffd.1901262