İn Vitro Çinko Uygulamasının DNA Hasarı, Lipid Peroksidasyonu ve Eritrosit Stabilitesi Üzerine Etkileri

Year 2019, , 316 - 326, 30.11.2019

Tuğba Demiral Muhammet Yusuf Tepebaşı , Furkan Calapoğlu Ayşe Bülbül Mustafa Calapoğlu

https://doi.org/10.29233/sdufeffd.436490

Abstract

Çinko; büyüme, hücre
bölünmesi, metabolizma, yara iyileşmesi, bağışıklık, üreme, tat ve görme
fonksiyonlarının işleyişi gibi birçok fizyolojik süreç için gerekli olan iki değerlikli bir geçiş iyondur. Çinko
maruziyetinin insan eritrosit zarının bazı özellikleri üzerindeki etkisi in vitro olarak çalışıldı. Ayrıca, insan
periferal kan lenfositlerinde, çinkonun genotoksik potansiyelini ortaya koymak için
alkali comet analizi yapılmıştır. Farklı derişimdeki tüm Zn²⁺
çözeltilerinin, doza bağımlı bir şekilde lipit oksidasyonunu inhibe etmede
oldukça zayıf bir etki göstermiştir. Eritrositlerin çinko ile inkübasyonunun
ardından, çinkonun hücrelerin hemolitik direncini belirgin bir şekilde azalmaya
yol açtığını da saptadık. Çalışmalarımız, yüksek derişimde çinkonun
insan eritrositleri için toksik olabileceğini ve hemolitik direncin değişmesine
neden olabildiğini göstermektedir. Comet sonuçları, kontrolle
karşılaştırıldığında yüksek dozlardaki çinko, doz-bağımlı olarak anlamlı
düzeyde DNA hasarı oluşturdu (p <0.05). Bu çalışmanın in vitro verileri yüksek dozda çinko alımının faydadan daha fazla
zarara neden olabileceğini düşündürmektedir.

Keywords

Çinko, Mebran stabilizasyonu, Genotoksisite, Lipid peroksidasyonu

Effects of In Vitro Zinc Treatment on the DNA Damage, Lipid Peroxidation and Erythrocyte Stability

Abstract

Zinc
is a divalent transition ion essential for many physiological processes
including: the growth and cell division, metabolism, wound healing, immunity,
reproduction, functioning of taste and eyesight. The effect of zinc exposure on
some properties of the human erythrocyte membrane was studied in vitro. In addition, the alkaline
comet assay was used to investigate genotoxicity potential of the zinc in the
peripheral blood lymphocytes. In this study, all the various concentrations of
Zn²⁺ solutions showed quite weak efficacy to inhibit lipid oxidation
in dose dependent manner. We also detected that incubation of erythrocytes with
zinc lead to the marked decrease of haemolytic resistance of the cells. Our
studies demonstrate that zinc at higher concentrations may be toxic to human
erythrocytes causing changes in the haemolytic resistance. The comet results
indicated a significant DNA damage at higher doses after treatment with zinc
when compared to controls showing a clear dose-dependent response (p<0.05).
The in vitro data of the present
study suggest that high dosage intake of zinc may cause more harm than benefit.

Keywords

Zinc, Membrane Stabilizing, Genotoxicity, Lipid peroxidation

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