İn Vitro Çinko Uygulamasının DNA Hasarı, Lipid Peroksidasyonu ve Eritrosit Stabilitesi Üzerine Etkileri

Abstract

Çinko; büyüme, hücre bölünmesi, metabolizma, yara iyileşmesi, bağışıklık, üreme, tat ve görme fonksiyonlarının işleyişi gibi birçok fizyolojik süreç için gerekli olan iki değerlikli bir geçiş iyondur. Çinko maruziyetinin insan eritrosit zarının bazı özellikleri üzerindeki etkisi in vitro olarak çalışıldı. Ayrıca, insan periferal kan lenfositlerinde, çinkonun genotoksik potansiyelini ortaya koymak için alkali comet analizi yapılmıştır. Farklı derişimdeki tüm Zn²⁺ çözeltilerinin, doza bağımlı bir şekilde lipit oksidasyonunu inhibe etmede oldukça zayıf bir etki göstermiştir. Eritrositlerin çinko ile inkübasyonunun ardından, çinkonun hücrelerin hemolitik direncini belirgin bir şekilde azalmaya yol açtığını da saptadık. Çalışmalarımız, yüksek derişimde çinkonun insan eritrositleri için toksik olabileceğini ve hemolitik direncin değişmesine neden olabildiğini göstermektedir. Comet sonuçları, kontrolle karşılaştırıldığında yüksek dozlardaki çinko, doz-bağımlı olarak anlamlı düzeyde DNA hasarı oluşturdu (p <0.05). Bu çalışmanın in vitro verileri yüksek dozda çinko alımının faydadan daha fazla zarara neden olabileceğini düşündürmektedir.

Keywords

• Çinko,Mebran stabilizasyonu,Genotoksisite,Lipid peroksidasyonu

Effects of In Vitro Zinc Treatment on the DNA Damage, Lipid Peroxidation and Erythrocyte Stability

Abstract

Zinc is a divalent transition ion essential for many physiological processes including: the growth and cell division, metabolism, wound healing, immunity, reproduction, functioning of taste and eyesight. The effect of zinc exposure on some properties of the human erythrocyte membrane was studied in vitro. In addition, the alkaline comet assay was used to investigate genotoxicity potential of the zinc in the peripheral blood lymphocytes. In this study, all the various concentrations of Zn²⁺ solutions showed quite weak efficacy to inhibit lipid oxidation in dose dependent manner. We also detected that incubation of erythrocytes with zinc lead to the marked decrease of haemolytic resistance of the cells. Our studies demonstrate that zinc at higher concentrations may be toxic to human erythrocytes causing changes in the haemolytic resistance. The comet results indicated a significant DNA damage at higher doses after treatment with zinc when compared to controls showing a clear dose-dependent response (p<0.05). The in vitro data of the present study suggest that high dosage intake of zinc may cause more harm than benefit.

Keywords

• Zinc,Membrane Stabilizing,Genotoxicity,Lipid peroxidation

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