Enzyme Immobilization and Optimization on Polymeric Nanospheres

Abstract

Enzymes are important materials that are used in many industries and they have a very high cost. Although the conditions for the enzymes in catalytic reactions are appropriate, enzymes can easily lose their activity in the presence of high temperature, high or low pH and organic / inorganic solvents. With the immobilization process, it is possible to ensure that the enzymes are economically usable. For this purpose, the glucose oxidase (GOx) enzyme was immobilized to aminomethyl polystyrene (APS) nanospheres and the optimization parameters (pH, temperature, reusability number, storage stability, substrate concentration) were determined. Michaelis-Menten equation for free and immobilized enzyme and K_m and V_max values were calculated. In particular, it has been found that the substrate of the immobilized enzyme containing Pt(IV) ion shows a high affinity for the substrate. After 12 months, immobilized enzymes were found to maintain about 85% of their activity.

Keywords

• GOx,Immobilization,Nanospheres

Polimerik Nanoküreler Üzerine Enzim İmmobilizasyonu ve Optimizasyonu

Abstract

Enzimler, pek çok endüstride kullanılan, maliyetleri oldukça yüksek olan önemli materyallerdir. Katalitik tepkimelerde enzimler için şartlar uygun olsa bile yüksek sıcaklık, yüksek veya düşük pH ve organik/inorganik çözücülerin varlığında enzimler aktifliklerini kolaylıkla kaybedebilirler. İmmobilizasyon işlemi ile birlikte, enzimlerin ekonomik açıdan kullanılabilirliğini sağlamak mümkündür. Bu amaçla, (aminometil) polistiren (APS) kaynaklı nanokürelere glukoz oksidaz (GOx) enzimi immobilize edildi ve optimizasyon parametreleri (pH, sıcaklık, tekrar kullanım sayısı, depolama kararlılığı, substrat derişimi) belirlendi. Serbest ve immobilize enzim için Michaelis-Menten eşitliği ile K_m ve V_mak değerleri hesaplandı. Özellikle Pt(IV) iyonu içeren immobilize enzimin substratına karşı yüksek ilgi gösterdiği tespit edildi. 12 ay sonunda immobilize enzimlerin yaklaşık % 85 aktifliklerini korudukları görüldü.

Keywords

• GOx,İmmobilizasyon,Nanoküreler

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