Dışsal Glisin Uygulamasıyla Allium cepa L.'da Tuz Teşvikli Stresin Hafifletilmesi

Abstract

Bu çalışmada, tuzluluğa maruz bırakılan Allium cepa L. tohumlarındaki fizyolojik parametreler olarak tohum çimlenmesi, radikula uzunluğu, radikula sayısı, taze ağırlık ve sitogenetik parametreler olarak mitotik aktivite, kromozomal anormallikler ve mikronükleus sıklığı üzerine glisinin rolü incelenmiştir. Tuzluluk A. cepa'nın tohum çimlenmesi ve fide büyümesinde önemli bir inhibe edici etki göstermiştir. Dahası, tuz A. cepa’nın kök ucu hücrelerindeki mitotik indeksi önemli ölçüde düşürmüş ve sitolojik hasarın en basit ve en etkili göstergesi olan mikronükleus sıklığı ve kromozomal anormalliklerin sayısını arttırmıştır. Buna karşılık, tohum çimlenmesi, mitotik aktivite, kromozomal anormallikler ve fide büyümesi üzerine tuzun inhibe edici etkileri glisin uygulamasıyla önemli ölçüde azalmış, fakat mikronükleus sıklığı üzerinde glisin tuz hasarının azaltılmasında yetersiz kalmıştır.

Keywords

• Glisin,Tohum çimlenmesi,Fide büyümesi,Mitotik indeks,Kromozomal anormallikler,Tuz stresi

Alleviation of Salt-Induced Stress in Allium cepa L. by Exogenous Glycine Treatment

Abstract

In this study, the role of glycine on the mitotic index, chromosome aberrations, micronucleus frequency as cytogenetic parameters and on the seed germination, radicle length, radicle number, fresh weight as physiological parameters in Allium cepa L. seeds exposed to saltness were studied. Salinity displayed a significant inhibitory effect on the seedling growth and seed germination of Allium cepa. Furthermore, salinity reduced significantly the mitotic index in A. cepa root tip cells and ascended number of chromosomal aberrations and frequency of micronucleus which is the simplest indicator, the most effective of cytological damage. On the other hand, the restricting effects of salinity on the seed germination, mitotic activity, chromosomal aberrations and seedling growth were alleviated dramatically in varying degrees by glycine application, but glycine was ineffective in reducing of salt damage on the micronucleus frequency.

Keywords

• Glycine,Seed germination,Seedling growth,Mitotic index,Chromosomal aberrations,Salt stress

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