Research Article
BibTex RIS Cite

EFFECTS OF 24-EPIBRASSINOLIDE ON SALINITY STRESS INDUCED INHIBITION OF SEED GERMINATION, SEEDLING GROWTH AND LEAF ANATOMY OF BARLEY

Year 2007, Volume: 2 Issue: 1, 41 - 52, 01.06.2007

Abstract

Amelioration of the inhibitory effect of salt stress (0.0, 0.30, 0.35 molal) on seed germination, seedling growth and leaf anatomy of barley (Hordeum vulgare L. cv. Bülbül 89) by infusing of 3 µM 24-epibrassinolide (EBR) to the seeds presowing for 24 hours was investigated. EBR pretreatment was found to be successful in ameliorating of the inhibitory effects of salt stress on final germination percentage, radicle elongation and fresh weight, while it observed to be unsuccessful on final coleoptile percentage and elongation. Besides, EBR pretreatment was mostly determined to have a succesful performance in ameliorating of the inhibitory effects of 0.30 and particularly 0.35 m salinity on leaf anatomy of barley seedlings. 

References

  • AL-KARAKI GN, 2001. Germination, sodium, and potassium concentrations of barley seeds as Influenced by salinity. Journal of Plant Nutrition, 24, 511-512.
  • ANURADHA S, RAO SSR, 2001. Effect of brassinosteroid on salinity stress induced inhibition of seed germination and seedling growth of rice (Oryza sativa L.). Plant Growth Regulation, 33, 151-153.
  • ANURADHA S, RAO SSR, 2003. Application of brassinosteroids to rice seeds (Oryza sativa L.) reduced the impact of salt stress on growth, prevented photosynthetic pigment loss and increased nitrate reductase activity. Plant Growth Regulation, 40, 29-32.
  • ARTECA JM, ARTECA RN, 2001. Brassinosteroid-induced exaggerated growth in hydroponically grown Arabidopsis plants. Physiologia Plantarum, 112, 104-112.
  • BAJGUZ A, 2000. Effect of brassinosteroids on nucleic acids and protein content in cultured cells of Chrorella vulgaris. Plant Physiology and Biochemistry, 38 (3), 209-215.
  • BOHNERT HJ, NELSON DE, JENSEN RG, 1995. Adaptations to enviromental stresses. Plant Cell, 7, 1099-1111.
  • BRAUN JW, KHAN AA, 1976. Alleviation of salinity and high temperature stress by plant growth regulators permeated into lettuce seeds via acetone. Journal of the American Society Horticultural Science, 101, 716-721.
  • BRAY S, REID DM, 2002. The effect of salinity and CO2 enrichment on the growth and anatomy of the second trifoliate leaf of Phaseolus vulgaris. Canadian Journal of Botany, 80, 349-359.
  • CLOUSE SD, HALL AF, LANGFORD M, MCMORRIS TC, BAKER ME, 1993. Physiological and molecular effects of brassinosteroids on Arabidopsis thaliana. Journal of Plant Growth Regulation, 12, 61-66.
  • CRAMER GR, QUARRIE SA, 2002. Abscisic acid is correlated with the leaf growth inhibition of four genotypes of maize differing in their response to salinity. Functional Plant Biology, 29, 111-115.
  • CURTIS PS, LAUCHLI A, 1987. The effect of moderate salt stress on leaf anatomy in Hibiscus cannabinus (Kenaf) and its relation to leaf area. American Journal of Botany, 74 (4), 538-542.
  • CAVUSOGLU K, 2006. Geleneksel hormonlarla son yıllarda bulunan bazı hormonların ve büyüme düzenleyicilerinin yüksek sıcaklık ve tuz (NaCI) stresleri altındaki arpa ve turp tohumlarının çimlenmesi üzerindeki etkilerinin karşılaştırılması. Doktora tezi, Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, 161 sayfa.
  • DASH M, PANDA SK, 2001. Salt stress induced changes in growth and enzyme activities in germinating Phaseolus mungo seeds. Biologia plantarum, 44, 587- 589.
  • EL-MASHAD AA, KAMEL EA, 2001. Amelioration of NaCl stress in Pisum sativum Linn. Indian Journal of Experimental Biology, 39, 469-475.
  • EUN JS, KURAISHI S, SAKURAI N, 1989. Changes in leaves and Suaeda monoica. Physiologia Plantarum, 46, 151-154.
  • FLOWERS TJ, HAJIBAGHERI MA, CLIPSON NTW, 1986. Halophytes. Quarterly Review of Biology, 61, 313-337.
  • GHOULAM C, FORES K, 2001. Effect of salinity on seed germination and early seedling growth of sugar beet (Beta vulgaris L.). Seed Science and Technology, 29, 357-364.
  • GULZAR S, KHAN MA, 2002. Alleviation of salinity-induced dormancy in perennial grasses. Biologia Plantarum, 45, 617-619.
  • HE RY, WANG GJ, WANG XS, 1991. Effect of Brassinolide on Growth and Chilling Resistance of Maize Seedlings. In: CUTLER HG. YOKOTA T. ADAM G. (Eds.) Brassinosteroids. Washington DC, American Chemical Society, pp. 220- 230.
  • HU Y, SCHMIDHALTER U, 2001. Reduced cellular cross-sectional area in the leaf elongation zone of wheat causes a decrease in dry weight deposition under saline conditions. Australian Journal of Plant Physiology, 28, 165-170.
  • HU YX, BAO F, LI JY, 2000. Promotive effects of brassinosteroids on cell division involves a distinct CycD3-induction pathway in Arabidopsis. The Plant Journal, 24, 693-701.
  • HUNTER WJ, 2001. Influence of root-applied epibrassinolide and carbenoxolone on the nodulation and growth of soybean (Glycine max L.) seedlings. Journal Agronomy Crop Science, 186, 217-221.
  • HWANG YH, CHEN SC, 1995. Anatomical responses in Kandelia candel (L.) druce seedlings growing in the presence of different concentrations of NaCI. Botanical Bulletin Academia Sinica, 36, 181-188.
  • JONES-HELD S, VAN DOREN M, LOOKWOOD T, 1996. Brassinolide application to Lepidium sativum seeds and the effects on seedling growth. Journal of Plant Growth Regulation, 15, 63-67.
  • KAMURO Y, TAKATSUTO S, 1991. Capability for Problems of Practical Uses of Brassinosteroids. In: CUTLER HG. YOKOTA T. ADAM G. (Eds.) Brassinosteroids-Chemistry, Bioactivity and Application, ACS Symp. Ser. 474, Washington DC, American Chemical Society, pp. 292-297.
  • KATSUMI M, 1991. Physiological Modes of Brassinolide Action in Cucumber Hypocotyl Growth. In: CUTLER HG. YOKOTA T. ADAM G. (Eds.) Brassinosteroids-Chemistry, Bioactivity and Applications, ACS Symp. Ser. 474, Washington DC, American Chemical Society, pp. 246-254.
  • KEMP PR, CUNNINGHAM GL, 1981. Temperature and salinity effects on growth leaf anatomy and photosynthesis of Distichlis spicata (L.) greene. American Journal of Botany, 68, 507-516.
  • LEUBNER-METZGER G, 2001. Brassinosteroids and gibberellins promote tobacco seed germination by distinct pathways. Planta, 203, 758-763.
  • MEIDNER H, MANSFIELD TA, 1968. Physiology of Stomata. Graw-Hill New York.
  • MITCHELL JW, MANDAVA NB, WORLEY JF, PLIMMER JR, SMITH MV, 1970. Brassins-a new family of plant hormones from rape pollen. Nature, 225, 1065- 1066.
  • MIZRAHI Y, BLUMONFELD A, BITTNER S, RICHMOND AE, 1971. Abscisic acid and cytokinin content of leaves in relation to salinity and relative humidity. Plant Physiology, 48, 752-755.
  • ONO EO, NAKAMURA T, MACHADO SR, RODRIGUES JD, 2000. Application of brassinosteroid to Tabebuia alba (Bignoniaceae) plants. Revista Brasileira de Fisiologia Vegetal, 12, 187-194.
  • OZDEMIR F, BOR, M, DEMIRAL T, TURKAN I, 2004. Effects of 24-epibrassinolid on seed germination, seedling growth, lipid peroxidation, proline content and antioxidative system of rice (Oryza sativa L.) under salinity stress. Plant Growth Regulation, 42, 203-211.
  • RAWSON HM, LONG MJ, MUNNS R, 1988. Growth and development in NaCI- treatedplants. I. leaf Na+ and CI- concentrations do not determine gas exchange of leaf blades in barley. Australian Journal of Plant Physiology, 15, 519-529.
  • ROBINSON SP, DOWNTON WJS, MILLHOUSE JA, 1983. Photosynthesis and ion content of leaves and isolated chloroplasts of salt-stressed spinach. Plant Physiology, 73 (2), 238-242.
  • SAIRAM RK, 1994. Effect of homobrassinolide application on plant metabolism and grain yield under irrigated and moisture-stress conditions of two wheat varieties. Plant Growth Regulation, 14, 173-181.
  • SASSE JM, 1985. The place of brassinolid in the sequential response to plant growth regulators in elongating tissue. Physiologia Plantarum, 63, 303-308.
  • SASSE JM, 1994. Brassinosteroid and roots. Process Plant Growth Regulation Society American, 21, 228-232.
  • SASSE JM, 1997. Recent progress in brassinosteroid research. Physiologia Plantarum, 100, 696-701.
  • SASSE JM, SMITH R, HUDSON I, 1995. Effect of 24-epibrassinolide on germination of seeds of Eucalyptus camaldulensis in saline conditions. Process Plant Growth Regulation Society American, 22, 136-141.
  • SCHILLING G, SCHILLER C, OTTO S, 1991. Influence of Brassinosteroids on Organ Relations and Enzyme Activities of Sugar Beet Plants. In: CUTLER HG. YOKOTA T. ADAM G. (Eds.) Brassinosteroids, Washington DC, American Chemical Society, pp. 208-230.
  • SHENNAN C, HUNT R, MACROBBIE EAC, 1987. Salt tolerance in Aster tripolium L. I. the effect of salinity on growth. Plant Cell and Environment, 10, 59-65.
  • SINGH J, NAKAMURA S, OTA Y, 1993. Effect of epibrassinolide on gram (Cicer arietinum) plants grown under water stress in juvenil stage. Indian Journal of Agricultural Sciences, 63, 395-397.
  • STEBER CM, MCCOURT P, 2001. A role for brassinosteroids in germination in Arabidopsis. Plant Physiology, 125, 763-769.
  • UNGAR IA, 1974. The effect of salinity and temperature on seed germination and growth of Hordeum jubatum. Canadian Journal of Botany, 52, 1357-1362.
  • WALKER MA, DUMBROFF EB, 1981. Effects of salt stress on abscisic acid and cytokinin levels in tomato. Pflanzenphy, 101, 461-470.
  • WILEN RW, SACCO M, GUSTA LW, KRISHNA P, 1995. Effects of 24- epibrassinolide on freezing and thermotolerance of bromegrass (Bromis inermis) cell cultures. Physiologia Plantarum, 95, 195-202.
  • YEO AR, LEE KS, IZARD P, BOURSIER PJ, FLOWERS TJ, 1991. Short-term and long-term effects of salinity on leaf growth in rice (Oryza sativa L.). Journal of Experimental Botany, 42, 881-889.
  • YOKOTA T, TAKAHASHI N, 1985. Chemistry, Physiology and Agricultural Application of Brassinolide and Related Steroids. In: BOPP M. (Ed.) Plant Growth Substances, Berlin, Springer-Verlag, pp. 129-138.

ARPANIN TOHUM ÇİMLENMESİ, FİDE BÜYÜMESİ VE YAPRAK ANATOMİSİNİN TUZ STRESİ TEŞVİKLİ İNHİBİSYONU ÜZERİNE 24- EPİBRASSİNOLİT’İN ETKİLERİ

Year 2007, Volume: 2 Issue: 1, 41 - 52, 01.06.2007

Abstract

Arpanın (Hordeum vulgare L. var. Bülbül 89) tohum çimlenmesi, fide büyümesi ve yaprak anatomisi üzerindeki tuz stresinin (0.0, 0.30, 0.35 molal) engelleyici etkisinin 3 µM 24-epibrassinolit’in (EBR) ekim öncesi tohumlara 24 saat infüzyonu vasıtası ile iyileştirilmesi araştırılmıştır. EBR ön muamelesi nihai çimlenme yüzdesi, radikula uzaması ve taze ağırlık üzerinde tuz stresinin engelleyici etkisini iyileştirmede başarılı olurken, nihai koleoptil yüzdesi ve uzaması üzerinde başarısız olduğu gözlenmiştir. Ayrıca EBR ön muamelesinin arpa fidelerinin yaprak anatomisi üzerinde 0.30 ve özellikle de 0.35 m tuzluluğun engelleyici etkisini iyileştirmede çoğunlukla başarılı bir performansa sahip olduğu tespit edilmiştir.

References

  • AL-KARAKI GN, 2001. Germination, sodium, and potassium concentrations of barley seeds as Influenced by salinity. Journal of Plant Nutrition, 24, 511-512.
  • ANURADHA S, RAO SSR, 2001. Effect of brassinosteroid on salinity stress induced inhibition of seed germination and seedling growth of rice (Oryza sativa L.). Plant Growth Regulation, 33, 151-153.
  • ANURADHA S, RAO SSR, 2003. Application of brassinosteroids to rice seeds (Oryza sativa L.) reduced the impact of salt stress on growth, prevented photosynthetic pigment loss and increased nitrate reductase activity. Plant Growth Regulation, 40, 29-32.
  • ARTECA JM, ARTECA RN, 2001. Brassinosteroid-induced exaggerated growth in hydroponically grown Arabidopsis plants. Physiologia Plantarum, 112, 104-112.
  • BAJGUZ A, 2000. Effect of brassinosteroids on nucleic acids and protein content in cultured cells of Chrorella vulgaris. Plant Physiology and Biochemistry, 38 (3), 209-215.
  • BOHNERT HJ, NELSON DE, JENSEN RG, 1995. Adaptations to enviromental stresses. Plant Cell, 7, 1099-1111.
  • BRAUN JW, KHAN AA, 1976. Alleviation of salinity and high temperature stress by plant growth regulators permeated into lettuce seeds via acetone. Journal of the American Society Horticultural Science, 101, 716-721.
  • BRAY S, REID DM, 2002. The effect of salinity and CO2 enrichment on the growth and anatomy of the second trifoliate leaf of Phaseolus vulgaris. Canadian Journal of Botany, 80, 349-359.
  • CLOUSE SD, HALL AF, LANGFORD M, MCMORRIS TC, BAKER ME, 1993. Physiological and molecular effects of brassinosteroids on Arabidopsis thaliana. Journal of Plant Growth Regulation, 12, 61-66.
  • CRAMER GR, QUARRIE SA, 2002. Abscisic acid is correlated with the leaf growth inhibition of four genotypes of maize differing in their response to salinity. Functional Plant Biology, 29, 111-115.
  • CURTIS PS, LAUCHLI A, 1987. The effect of moderate salt stress on leaf anatomy in Hibiscus cannabinus (Kenaf) and its relation to leaf area. American Journal of Botany, 74 (4), 538-542.
  • CAVUSOGLU K, 2006. Geleneksel hormonlarla son yıllarda bulunan bazı hormonların ve büyüme düzenleyicilerinin yüksek sıcaklık ve tuz (NaCI) stresleri altındaki arpa ve turp tohumlarının çimlenmesi üzerindeki etkilerinin karşılaştırılması. Doktora tezi, Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, 161 sayfa.
  • DASH M, PANDA SK, 2001. Salt stress induced changes in growth and enzyme activities in germinating Phaseolus mungo seeds. Biologia plantarum, 44, 587- 589.
  • EL-MASHAD AA, KAMEL EA, 2001. Amelioration of NaCl stress in Pisum sativum Linn. Indian Journal of Experimental Biology, 39, 469-475.
  • EUN JS, KURAISHI S, SAKURAI N, 1989. Changes in leaves and Suaeda monoica. Physiologia Plantarum, 46, 151-154.
  • FLOWERS TJ, HAJIBAGHERI MA, CLIPSON NTW, 1986. Halophytes. Quarterly Review of Biology, 61, 313-337.
  • GHOULAM C, FORES K, 2001. Effect of salinity on seed germination and early seedling growth of sugar beet (Beta vulgaris L.). Seed Science and Technology, 29, 357-364.
  • GULZAR S, KHAN MA, 2002. Alleviation of salinity-induced dormancy in perennial grasses. Biologia Plantarum, 45, 617-619.
  • HE RY, WANG GJ, WANG XS, 1991. Effect of Brassinolide on Growth and Chilling Resistance of Maize Seedlings. In: CUTLER HG. YOKOTA T. ADAM G. (Eds.) Brassinosteroids. Washington DC, American Chemical Society, pp. 220- 230.
  • HU Y, SCHMIDHALTER U, 2001. Reduced cellular cross-sectional area in the leaf elongation zone of wheat causes a decrease in dry weight deposition under saline conditions. Australian Journal of Plant Physiology, 28, 165-170.
  • HU YX, BAO F, LI JY, 2000. Promotive effects of brassinosteroids on cell division involves a distinct CycD3-induction pathway in Arabidopsis. The Plant Journal, 24, 693-701.
  • HUNTER WJ, 2001. Influence of root-applied epibrassinolide and carbenoxolone on the nodulation and growth of soybean (Glycine max L.) seedlings. Journal Agronomy Crop Science, 186, 217-221.
  • HWANG YH, CHEN SC, 1995. Anatomical responses in Kandelia candel (L.) druce seedlings growing in the presence of different concentrations of NaCI. Botanical Bulletin Academia Sinica, 36, 181-188.
  • JONES-HELD S, VAN DOREN M, LOOKWOOD T, 1996. Brassinolide application to Lepidium sativum seeds and the effects on seedling growth. Journal of Plant Growth Regulation, 15, 63-67.
  • KAMURO Y, TAKATSUTO S, 1991. Capability for Problems of Practical Uses of Brassinosteroids. In: CUTLER HG. YOKOTA T. ADAM G. (Eds.) Brassinosteroids-Chemistry, Bioactivity and Application, ACS Symp. Ser. 474, Washington DC, American Chemical Society, pp. 292-297.
  • KATSUMI M, 1991. Physiological Modes of Brassinolide Action in Cucumber Hypocotyl Growth. In: CUTLER HG. YOKOTA T. ADAM G. (Eds.) Brassinosteroids-Chemistry, Bioactivity and Applications, ACS Symp. Ser. 474, Washington DC, American Chemical Society, pp. 246-254.
  • KEMP PR, CUNNINGHAM GL, 1981. Temperature and salinity effects on growth leaf anatomy and photosynthesis of Distichlis spicata (L.) greene. American Journal of Botany, 68, 507-516.
  • LEUBNER-METZGER G, 2001. Brassinosteroids and gibberellins promote tobacco seed germination by distinct pathways. Planta, 203, 758-763.
  • MEIDNER H, MANSFIELD TA, 1968. Physiology of Stomata. Graw-Hill New York.
  • MITCHELL JW, MANDAVA NB, WORLEY JF, PLIMMER JR, SMITH MV, 1970. Brassins-a new family of plant hormones from rape pollen. Nature, 225, 1065- 1066.
  • MIZRAHI Y, BLUMONFELD A, BITTNER S, RICHMOND AE, 1971. Abscisic acid and cytokinin content of leaves in relation to salinity and relative humidity. Plant Physiology, 48, 752-755.
  • ONO EO, NAKAMURA T, MACHADO SR, RODRIGUES JD, 2000. Application of brassinosteroid to Tabebuia alba (Bignoniaceae) plants. Revista Brasileira de Fisiologia Vegetal, 12, 187-194.
  • OZDEMIR F, BOR, M, DEMIRAL T, TURKAN I, 2004. Effects of 24-epibrassinolid on seed germination, seedling growth, lipid peroxidation, proline content and antioxidative system of rice (Oryza sativa L.) under salinity stress. Plant Growth Regulation, 42, 203-211.
  • RAWSON HM, LONG MJ, MUNNS R, 1988. Growth and development in NaCI- treatedplants. I. leaf Na+ and CI- concentrations do not determine gas exchange of leaf blades in barley. Australian Journal of Plant Physiology, 15, 519-529.
  • ROBINSON SP, DOWNTON WJS, MILLHOUSE JA, 1983. Photosynthesis and ion content of leaves and isolated chloroplasts of salt-stressed spinach. Plant Physiology, 73 (2), 238-242.
  • SAIRAM RK, 1994. Effect of homobrassinolide application on plant metabolism and grain yield under irrigated and moisture-stress conditions of two wheat varieties. Plant Growth Regulation, 14, 173-181.
  • SASSE JM, 1985. The place of brassinolid in the sequential response to plant growth regulators in elongating tissue. Physiologia Plantarum, 63, 303-308.
  • SASSE JM, 1994. Brassinosteroid and roots. Process Plant Growth Regulation Society American, 21, 228-232.
  • SASSE JM, 1997. Recent progress in brassinosteroid research. Physiologia Plantarum, 100, 696-701.
  • SASSE JM, SMITH R, HUDSON I, 1995. Effect of 24-epibrassinolide on germination of seeds of Eucalyptus camaldulensis in saline conditions. Process Plant Growth Regulation Society American, 22, 136-141.
  • SCHILLING G, SCHILLER C, OTTO S, 1991. Influence of Brassinosteroids on Organ Relations and Enzyme Activities of Sugar Beet Plants. In: CUTLER HG. YOKOTA T. ADAM G. (Eds.) Brassinosteroids, Washington DC, American Chemical Society, pp. 208-230.
  • SHENNAN C, HUNT R, MACROBBIE EAC, 1987. Salt tolerance in Aster tripolium L. I. the effect of salinity on growth. Plant Cell and Environment, 10, 59-65.
  • SINGH J, NAKAMURA S, OTA Y, 1993. Effect of epibrassinolide on gram (Cicer arietinum) plants grown under water stress in juvenil stage. Indian Journal of Agricultural Sciences, 63, 395-397.
  • STEBER CM, MCCOURT P, 2001. A role for brassinosteroids in germination in Arabidopsis. Plant Physiology, 125, 763-769.
  • UNGAR IA, 1974. The effect of salinity and temperature on seed germination and growth of Hordeum jubatum. Canadian Journal of Botany, 52, 1357-1362.
  • WALKER MA, DUMBROFF EB, 1981. Effects of salt stress on abscisic acid and cytokinin levels in tomato. Pflanzenphy, 101, 461-470.
  • WILEN RW, SACCO M, GUSTA LW, KRISHNA P, 1995. Effects of 24- epibrassinolide on freezing and thermotolerance of bromegrass (Bromis inermis) cell cultures. Physiologia Plantarum, 95, 195-202.
  • YEO AR, LEE KS, IZARD P, BOURSIER PJ, FLOWERS TJ, 1991. Short-term and long-term effects of salinity on leaf growth in rice (Oryza sativa L.). Journal of Experimental Botany, 42, 881-889.
  • YOKOTA T, TAKAHASHI N, 1985. Chemistry, Physiology and Agricultural Application of Brassinolide and Related Steroids. In: BOPP M. (Ed.) Plant Growth Substances, Berlin, Springer-Verlag, pp. 129-138.
There are 49 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Makaleler
Authors

Semra Kılıç This is me

Kürşat Çavuşoğlu This is me

Kudret Kabar This is me

Publication Date June 1, 2007
Published in Issue Year 2007 Volume: 2 Issue: 1

Cite

IEEE S. Kılıç, K. Çavuşoğlu, and K. Kabar, “EFFECTS OF 24-EPIBRASSINOLIDE ON SALINITY STRESS INDUCED INHIBITION OF SEED GERMINATION, SEEDLING GROWTH AND LEAF ANATOMY OF BARLEY”, Süleyman Demirel University Faculty of Arts and Science Journal of Science, vol. 2, no. 1, pp. 41–52, 2007.