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VICIA FABA L. (FABACEAE) KÖK UCU HÜCRELERİNDE FENOL TARAFINDAN TEŞVİK EDİLEN SİTOTOKSİSİTENİN BELİRLENMESİ

Year 2008, Volume: 3 Issue: 2, 139 - 148, 01.12.2008

Abstract

Özet: Bu çalışmada, Vicia faba L. kök ucu hücrelerine fenol'ün farklı
konsantrasyonlarının sitotoksik etkileri araştırıldı. Test materyali olarak bakla tohumları
kullanıldı. Çimlenme yüzdesi, kök uzunluğu ve mikronukleus (MN) sıklığı
sitotoksisitenin indikatörleri olarak kullanıldı ve bu veriler istatistiksel parametreler ile
ilişkilendirildi. Sitogenetik analizlere ilaveten, fenol ile muamele edilen bakla
tohumlarının kök ucu meristemlerinde DNA analizleri gerçekleştirildi. Tohumlar
kontrol ve fenol uygulama grupları olarak iki gruba ayrıldı ve 7 gün süresince fenol'ün
üç farklı dozu (25, 50 ve 75 ppm) ile muamele edildi. Sonuçta, fenol'ün tüm uygulama
grubu tohumlarda doza bağlı olarak çimlenme yüzdesi, kök uzunluğu ve ağırlık
kazanımını azalttığı, MN oranını ise attırdığı tespit edildi. Ayrıca, fenol ile muamele
edilen tohumlarda DNA ürünü kontrol grubundakilerden daha düşüktü. Bu nedenle,
fenole maruz kalan DNA ürünleri agaroz jelde kontrol grubuna göre daha ileride bantlar
verdi. Sonuç olarak, elde edilen veriler fenol'ün bakla kök ucu hücreleri üzerinde
sitotoksik etkiye sahip olduğunu gösterdi.
Anahtar kelimeler: Fenol sitotoksisite, mikronukleus testi, tohum çimlenmesi, Vicia
faba L.
DETECTION OF CYTOTOXICITY INDUCED BY PHENOL IN ROOT TIP
CELLS OF VICIA FABA L. (FABACEAE)
Abstract: In this study, cytotoxic effects of different concentrations of phenol on Vicia
faba L. root tips were investigated. The test material was used the seeds of broad bean.
It was used germination percentage, root lenght, weight gain and micronucleus (MN)
frequency as indicators of cytotoxicity, and correlate these data with statistical
parameters. Additionally to the cytogenetic analysis, DNA analyses were performed
from root tip meristem of broad bean treated with phenol. The seeds were divided into
two groups: control, phenol treatment groups, and they were treated with different three
doses (25, 50 and 75 ppm) of phenol during 7 days. As s result, it was determined that
phenol fairly decreased the germination percentage, root length and weight gain
depending on dose in seeds all treatment groups, whereas MN rate was increased.
Besides, it was observed that the yield of DNA in seeds treated with phenol were lower
than recorded in the control. Hence, DNA yields exposed to phenol was run ahead on
agarose gel according to control group. In conclusion, obtained date indicated that
phenol had cytotoxic effects on broad bean root tip cells.
Key words: Phenol toxicity, micronucleus test, seed cytogenetic, Vicia faba L.

References

  • ATİK M, KARAGÜZEL O, ERSOY S, 2007. Sıcaklığın Dalbergia sissoo tohumlarının çimlenme özelliklerine etkisi. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 20 (2), 203–210.
  • BURTON KW, MORGAN E, ROIG A, 1984. The influence of heavy metals on the growth of sitka-spruce in South wales forests. II green house experiments. Plant Soil, 78, 271–282.
  • CANADIAN ENVIRONMENTAL, 2000. Priority substances list assessment report. Phenol Protection Act, pp. 1–73 (erişim:http://www.hc-sc.gc.ca/ewh-semt/alt).
  • DOBBINS DC, THORNTON-MANNING J, JONES DD, FEDERLE TW, 1987. Mineralization potential for phenol in subsurface soils. Journal of Environmental Quality, 16, 54–58
  • ENVIRONMENT CANADA, 1998. Priority Substances List supporting document for the environmental assessment of phenol. Canadian Environmental Protection Act. Commercial Chemicals Evaluation Branch, Hull, Quebec, pp.1–7.
  • FENECH M, CHANG WP, KIRSCH-VOLDERS M, HOLLAND N, BONASSI S, ZEIGER E, 2003. Human MicronNucleus project. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutation Research, 534, 65– 75.
  • İNCEER H, AYAZ S, BEYAZOĞLU O, ŞENTÜRK E, 2003. Cytogenetic effects of copper chloride on the root tip cells of Helianthus annuus L. Turkish Journal of Biology, 27, 43–46.
  • KARK P, 1979. Clinical and neurochemical aspects of inorganic mercury intoxication, In Handbook of Clinical Neurology. Elsevier, pp.147–197.
  • LIU J, REID RJ, SMITH FA, 2000. The mechanism of cobalt toxicity in mung beans. Physiologia Plantarum, 110, 104–110.
  • MCFARLANE JC, PFLEEGER T, FLETCHER J, 1987. Transpiration Effect on the Uptake and Distribution of Bromacil, Nitrobenzene, and Phenol in Soybean Plants. Journal of Environmental Quality, 16, 372–376.
  • MOZDARANI H, KAMALI S, 1998. Antigenotoxic effects of cimetidine against benzene induced micronuclei in mouse bone marrow erythrocytes. Toxicology Letters, 99, 53–61.
  • MUSCOLO A, PANUCCIO MR, SIDARI M, 2001. The effect of phenols on res- piratory enzymes in seed germination. Plant Growth Regulation, 35, 31–35.
  • ÖZYİGİT İİ, KAHRAMANMV, ERCAN Ö, 2007. Relation between explant age, total phenols and regeneration response in tissue cultured cotton (Gossypium hirsutum L.). African Journal of Biotechnology, 6 (1), 3–8.
  • PIETRASZEWSKA TM, 2001. Effect of aluminium on plant growth and metabolism. Acta Biochimica Polanica, 48 (3), 673–686.
  • SHARMA AD, Gill PK, SINGH P, 2002. DNA Isolation From Dry And Fresh Samples of Polysaccharide-Rich Plants. Plant Molecular Biology Reporter, 20, 415–415.
  • SHARMA P, DUBEY S, 2005. Lead toxicity in plants. Brazilian Journal of Plant Physiology, 17, 35–52.
  • STAYKOVA TA, IVANOVA EN, VELCHEVA IG, 2005. Cytogenetic effect of heavy metal and cyanide in contamined waters from the region of southwest Bulgaria. Journal of Cell and Molecular Biology, 4, 41–46.
  • UGREKHELIDZE D, KVESİTADZE G, ARZIANI B, MITHAISHVILI T, PHIRIASHVILI V, 1999. Detoxication of phenol in annual plant seedlings. Ecotoxicology and Environmental Safety, 42 (2), 119–124.
  • WALLSTEDT A, SOMMARIN M, NILSSON MC, MUNSON AD, MARGOLIS HA, 2001. The inhibition of ammonium uptake in excised birch (Betula pendula) roots by batatasin-III. Physiologia Plantarium, 113, 368–376.
  • WEI QX, 2004. Mutagenic effects of chromium trioxide on root tip cells of Vicia faba. Journal of Zhejiang University Science, 5, 1570–1576.
  • WEINBERGER P, VLADUT R, 1981. Comparative toxic effects of some xenebiotics on the germination and early seedling growth of jack pine (Pinus banksiana Lamb.) and white birch (Betula papyrifera March). Canadian Journal of Forestry Research, 11, 796–804.
  • YENER J, AKSU Z, 1999. Atıksulardaki fenol ve klorofenollerin aktif karbon ve kurutulmuş aktif çamura adsorpsiyonu. Turkish Journal of Engineering and Environmental Science, 23, 93–104.
Year 2008, Volume: 3 Issue: 2, 139 - 148, 01.12.2008

Abstract

References

  • ATİK M, KARAGÜZEL O, ERSOY S, 2007. Sıcaklığın Dalbergia sissoo tohumlarının çimlenme özelliklerine etkisi. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 20 (2), 203–210.
  • BURTON KW, MORGAN E, ROIG A, 1984. The influence of heavy metals on the growth of sitka-spruce in South wales forests. II green house experiments. Plant Soil, 78, 271–282.
  • CANADIAN ENVIRONMENTAL, 2000. Priority substances list assessment report. Phenol Protection Act, pp. 1–73 (erişim:http://www.hc-sc.gc.ca/ewh-semt/alt).
  • DOBBINS DC, THORNTON-MANNING J, JONES DD, FEDERLE TW, 1987. Mineralization potential for phenol in subsurface soils. Journal of Environmental Quality, 16, 54–58
  • ENVIRONMENT CANADA, 1998. Priority Substances List supporting document for the environmental assessment of phenol. Canadian Environmental Protection Act. Commercial Chemicals Evaluation Branch, Hull, Quebec, pp.1–7.
  • FENECH M, CHANG WP, KIRSCH-VOLDERS M, HOLLAND N, BONASSI S, ZEIGER E, 2003. Human MicronNucleus project. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutation Research, 534, 65– 75.
  • İNCEER H, AYAZ S, BEYAZOĞLU O, ŞENTÜRK E, 2003. Cytogenetic effects of copper chloride on the root tip cells of Helianthus annuus L. Turkish Journal of Biology, 27, 43–46.
  • KARK P, 1979. Clinical and neurochemical aspects of inorganic mercury intoxication, In Handbook of Clinical Neurology. Elsevier, pp.147–197.
  • LIU J, REID RJ, SMITH FA, 2000. The mechanism of cobalt toxicity in mung beans. Physiologia Plantarum, 110, 104–110.
  • MCFARLANE JC, PFLEEGER T, FLETCHER J, 1987. Transpiration Effect on the Uptake and Distribution of Bromacil, Nitrobenzene, and Phenol in Soybean Plants. Journal of Environmental Quality, 16, 372–376.
  • MOZDARANI H, KAMALI S, 1998. Antigenotoxic effects of cimetidine against benzene induced micronuclei in mouse bone marrow erythrocytes. Toxicology Letters, 99, 53–61.
  • MUSCOLO A, PANUCCIO MR, SIDARI M, 2001. The effect of phenols on res- piratory enzymes in seed germination. Plant Growth Regulation, 35, 31–35.
  • ÖZYİGİT İİ, KAHRAMANMV, ERCAN Ö, 2007. Relation between explant age, total phenols and regeneration response in tissue cultured cotton (Gossypium hirsutum L.). African Journal of Biotechnology, 6 (1), 3–8.
  • PIETRASZEWSKA TM, 2001. Effect of aluminium on plant growth and metabolism. Acta Biochimica Polanica, 48 (3), 673–686.
  • SHARMA AD, Gill PK, SINGH P, 2002. DNA Isolation From Dry And Fresh Samples of Polysaccharide-Rich Plants. Plant Molecular Biology Reporter, 20, 415–415.
  • SHARMA P, DUBEY S, 2005. Lead toxicity in plants. Brazilian Journal of Plant Physiology, 17, 35–52.
  • STAYKOVA TA, IVANOVA EN, VELCHEVA IG, 2005. Cytogenetic effect of heavy metal and cyanide in contamined waters from the region of southwest Bulgaria. Journal of Cell and Molecular Biology, 4, 41–46.
  • UGREKHELIDZE D, KVESİTADZE G, ARZIANI B, MITHAISHVILI T, PHIRIASHVILI V, 1999. Detoxication of phenol in annual plant seedlings. Ecotoxicology and Environmental Safety, 42 (2), 119–124.
  • WALLSTEDT A, SOMMARIN M, NILSSON MC, MUNSON AD, MARGOLIS HA, 2001. The inhibition of ammonium uptake in excised birch (Betula pendula) roots by batatasin-III. Physiologia Plantarium, 113, 368–376.
  • WEI QX, 2004. Mutagenic effects of chromium trioxide on root tip cells of Vicia faba. Journal of Zhejiang University Science, 5, 1570–1576.
  • WEINBERGER P, VLADUT R, 1981. Comparative toxic effects of some xenebiotics on the germination and early seedling growth of jack pine (Pinus banksiana Lamb.) and white birch (Betula papyrifera March). Canadian Journal of Forestry Research, 11, 796–804.
  • YENER J, AKSU Z, 1999. Atıksulardaki fenol ve klorofenollerin aktif karbon ve kurutulmuş aktif çamura adsorpsiyonu. Turkish Journal of Engineering and Environmental Science, 23, 93–104.
There are 22 citations in total.

Details

Primary Language Turkish
Journal Section Makaleler
Authors

Kültiğin Çavuşoğlu

Publication Date December 1, 2008
Published in Issue Year 2008 Volume: 3 Issue: 2

Cite

IEEE K. Çavuşoğlu, “VICIA FABA L. (FABACEAE) KÖK UCU HÜCRELERİNDE FENOL TARAFINDAN TEŞVİK EDİLEN SİTOTOKSİSİTENİN BELİRLENMESİ”, Süleyman Demirel University Faculty of Arts and Science Journal of Science, vol. 3, no. 2, pp. 139–148, 2008, doi: 10.29233/sdufeffd.134650.