Biyolojik Örneklerde Elektrotermal Atomik Absorpsiyon Spektroskopisi ile Kurşun Tayininde Nitrik Asitin ve Bazı Ortam Düzenleyicilerin Etkisinin İncelenmesi

Year 2012, Volume: 7 Issue: 1, 23 - 32, 04.06.2012

Ramazan Duman Selda Doğan , F. Nazlı Dinçer Kaya

Abstract

Özet: Bu çalışmada, elektrotermal atomik absorpsiyon spektroskopisi (ETAAS) ile biyolojik örneklerde kurşun tayininde nitrik asit ve bazı ortam düzenleyicilerin etkileri incelendi. Farklı nitrik asit derişimleri kullanılarak Pt + Pd, Pd + Mg(NO3)2 ve NH4H2PO4 + Mg(NO3)2 ortamlarında piroliz ve atomlaşma sıcaklığı optimizasyon çalışmaları yapıldı. % 10 HNO3 + NH4H2PO4 + Mg(NO3)2 ortamı en uygun ortam olarak belirlendi. Yöntemin doğruluğu CRM BCR-397 insan saçı, NIST 2976 midye dokusu ve HIGH EP-H-1 içme suyu standart referans maddeleri ile kontrol edildi. Ölçülen değerlerin, sertifika değerlerinden sapmasını göstermek için bağıl hata hesaplandı ve belirlenen ortamda üç standart referans madde için bağıl hata % 10'un altında bulundu. Belirlenen koşullarda kurşunun gözlenebilme sınırı (LOD) 0,67 μg/L, tayin sınırı (LOQ) 2,01 μg/L olarak hesaplandı.

Anahtar kelimeler: Kurşun tayini, ortam düzenleyici, elektrotermal atomik absorpsiyon, LOQ

Keywords

Kurşun tayini, ortam düzenleyici, elektrotermal atomik absorpsiyon, LOQ

Investigation of Effect of Nitric Acid and Various Matrix Modifier on Determination of Lead in Biological Samples by Electrothermal Atomic Absorption Spectroscopy

Abstract

Abstract: In this study, determination of lead in biological materials
by electrothermal atomic absorption spectroscopy was investigated.
Pyrolysis and atomization temperatures were optimized in Pt + Pd, Pd +
Mg(NO3)2 and NH4H2PO4 + Mg(NO3)2 mixtures using various nitric acid
concentrations. % 10 HNO3 + NH4H2PO4 + Mg(NO3)2 mixtures was designated
as the best conditions. The accuracy of the method was tested by
certificated reference standard materials, CRM BCR-397 human hair, NIST
2976 mussel tissue and HIGH EP-H-1 drinking water. Relative errors were
less than 10 % for all of the certificated reference standard materials.
Limit of detection (LOD) and limit of quantification (LOQ) values were
calculated as 0,67 μg/L and 2,01.

Keywords

Lead determination, matrix modifier, electrothermal atomic absorption spectroscopy, LOQ

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