Şarap Örneklerinde Sıvı-Sıvı Ekstraksiyonu İle TransResveratrol Analizi

Year 2013, Volume: 8 Issue: 1, 114 - 121, 10.06.2013

Emine İnal Sevi Öz Arda Atakol Mehmet Akay Orhan Atakol

Abstract

3,5,4-trans-trihidroksistilben (trans-resveratrol)’in CH2Cl2 ve CHCl3 içindeki çözünürlüğü oldukça fazladır. Trans-resveratrolü tam ekstrakte etmek için, şarap örnekleri CHCl3 içinde çalkalandı. Elde edilen CHCl3 çözeltisi HPLC sistemine direk olarak enjekte edildi. Analiz, %60 MeCN: %40 EtOH içinde ve trans-resveratrol standart eklenmesiyle gerçekleştirildi. Kromatogramdaki rezolüsyon yaklaşık 1.0 olarak hesaplandı. Türkiye de üretilmiş olan 9 şarap örneğinde resveratrol konsantrasyonu 1.46-6.53 ppm olarak bulundu. Resveratrolün ekstraksiyon oranı, ardışık ekstraksiyon ile tespit edildi ve örnek miktarı arttırılarak, metodun doğruluğu test edildi. Geri kazanım % 100.42 olarak bulundu.

Keywords

Resveratrol tayini, şarap, HPLC, sıvı-sıvı ekstraksiyonu

Liquid-Liquid Extraction Based Simple Trans-Resveratrol Analysis In Wine Samples

Abstract

3,5,4-trans-trihydroxystilbene (trans-resveratrol) is quite soluble in CH2Cl2 and CHCl3. Wine samples are shaken in and rinsed with CHCl3 to obtain fine extracts of trans-resveratrol. The obtained CHCl3 solutions were directly injected to HPLC system for analysis. Determinations were made in 60% MeCN: 40% EtOH, and by trans-resveratrol standard addition. The resolution is calculated to be about 1.0 in the chromatogram. In 9 wine samples produced in Turkey, resveratrol concentrations were detected in between 1.46 and 6.53 ppm. By consecutive extractions, extraction ratio of resveratrol was determined and by increasing the amount of the samples, accuracy of the method was tested. It was found that the recovery is about 100.42 %

Keywords

Resveratrol determination, wine, HPLC, liquid-liquid extraction

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