Gökkuşağı Alabalığı (Oncorhynchus mykiss,W., 1792) Solungaç Mukus Hücrelerindeki Glikokonjugatların Histokimyasal Analizi

Year 2014, Volume: 9 Issue: 1, 6 - 11, 15.07.2014

Nurgül Şenol , Özlem Yeşil

Abstract

Bu çalışmada histokimyasal tekniklerle gökkuşağı alabalığı (Oncorhynchus mykiss) solungaç dokusunun farklı bölgelerindeki glikokonjugatların belirlenmesi amaçlanmıştır. Solungaçlardan örnek alımı gerçekleştirildi. Örnekler rutin histolojik doku takibinden geçirilip parafinde bloklandı. Farklı karbonhidrat gruplarının yoğunluğunu belirlemek için histokimyasal boyama yöntemleri uygulandı. Yapılan histokimyasal gözlemlerde solungaç bölgelerinin tümünde nötral (PAS), asidik (AB pH 2.5), sulfatlı (AF), nötral yada asidik (PAS/AB pH 2.5), güçlü sülfatlı (AF/AB pH 2.5) gilikoproteinler belirlendi. 

Keywords

Glikokonjugat, gökkuşağı alabalığı, Oncorhynchus mykiss, solungaç

Histochemical Analysis of Glycoconjugates in the Mucous Cells in the Gill of Rainbow trout (Oncorhynchus mykiss,W., 1792)

Abstract

In the present observation, histochemical methods were utilized to understand the structural organization and to categorize the diverse classes of glycoproteins eleborated by the epithelium in various regions of gills of a Rainbow trout, Oncorhynchus mykiss. Samples were taken from the gills. The samples were routinely processed and embedded in parafin. Histochemical techniques were performed for the density and differentiation of carbohydrate moieties. The presence of neutral (PAS), acidic (AB pH 2.5) and sulphate (AF), neutral and/or acid rich (PAS/AB pH 2.5), strong sulphated (AF/AB pH 2.5) of glycoconjugates were identified by means of conventional histochemistry in all regions

Keywords

Glycoconjugates, gill, Oncorhynchus mykiss, rainbow trout

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