Development of a Method for the Analysis of Polycyclic Aromatic Hydrocarbons Using FMS-TTP-SPE Pretreatment System: Application to Water Samples of Gölcük Lake in Isparta Province

Year 2023, Volume: 18 Issue: 2, 170 - 180, 22.06.2023

Murat Kılıç , Ömer Dilek

https://doi.org/10.29233/sdufeffd.1218334

Abstract

It is known that the carcinogenic effects of Polycyclic Aromatic Hydrocarbon (PAH) compounds are high. Therefore, accurate determination of PAH concentrations in water is an important research topic. Since PAHs have low solubility values in water (nanogram/liter), sensitive analytical techniques and sample pretreatment systems are required in order to determine the low levels of PAH compounds in water samples. One of these techniques is the extraction of PAHs from environmental water samples using the Fluid Management Systems- Turbo Trace Parallel-Solid Phase Extraction (FMS-TTP-SPE) pretreatment system. In this study, a chromatographic analysis and extraction method was validated and adopted for the extraction of 11 PAH compounds in water samples using the FMS-TTP-SPE pretreatment system and a gas chromatography equipped with a flame ionization detector (GC-FID). From the results, the LOD values were calculated between 6.5 and 13.7, and the LOQ values between 21.6 and 45.6 µg L-1. Recovery (R) results were changed between 85.0% and 97.0%. Concerning the proposed method, it has been shown that high concentrations of PAHs can be obtained in a single extraction procedure compared to conventional approaches. Using the developed method, twelve surface lake water samples were collected in April 2022 from Gölcük lake which is a volcanic lake. Eleven PAH compounds were investigated in the lake water samples using the proposed method.

Keywords

PAH, FMS Turbo Trace Paralel SPE System, GC-FID, Method Validation

FMS-TTP-SPE Ön Hazırlık Sistemi Kullanılarak Polisiklik Aromatik Hidrokarbonların Analizi için Yöntem Geliştirilmesi: Yöntemin Isparta İli Gölcük Gölünden Alınan Su Numunelerine Uygulanması

Abstract

Polisiklik Aromatik Hidrokarbon (PAH) bileşiklerinin kanserojen etkilerinin yüksek olduğu bilinmektedir. PAH’ların sulardaki çözünürlük değerlerinin düşük (nanogram/litre) olması nedeni ile PAH tayini ön hazırlık işlemlerinin doğru yapılması ve farklı analitik cihazlar ile düşük konsantrasyonlardaki tespitlerinin belirlenmesi için hassas analitik teknikler gereklidir. Bu tekniklerden bir tanesi de Akışkan Yönetim Sistemleri- Turbo Trace Paralel- Katı Faz Ekstraksiyonu (Fluid Management Systems - Turbo Trace Parallel - Solid Phase Extraction, FMS-TTP-SPE) ön hazırlık sistemi kullanılarak çevresel su numunelerinden PAH'ların ekstraksiyonudur. Bu çalışmada, FMS-TTP-SPE ön hazırlık sistemi kullanarak su numunelerinde bulunan 11 adet PAH bileşiğinin ekstraksiyonu ve sonrasında konsantrasyonlarının gaz kromatografi cihazında (GC-FID) belirlenmesi için kromatografik analiz ve ekstraksiyon metodu valide edilmiştir. Gaz kromatografisi ile konsantrasyon belirleme çalışmalarının doğrulama parametreleri için elde edilen sonuçlardan LOD değerleri 6,48 ile 13,69 arasında, LOQ değerleri ise 21,60 ile 45,62 µg L-1 arasında hesaplanmıştır. Geri kazanım (GK) testleri ise %85,0 ile %97,0 arasında tespit edilmiştir. Önerilen yöntem ile, geleneksel yaklaşımlara kıyasla tek bir ekstraksiyon prosedüründe çok düşük PAH konsantrasyonlarının tespit edilebildiği gösterilmiştir. Geliştirilen metot kullanılarak; 2022 yılı Nisan ayı içerisinde, volkanik göl olan Gölcük gölünden on iki adet göl suyu örneği toplanmış ve PAH’ların konsantrasyon tespit çalışmaları yapılmıştır.

Keywords

PAH, FMS Turbo Trace Paralel SPE Sistem, GC-FID, Metot Validasyon

Supporting Institution

ISPARTA UYGULAMALI BİLİMLER ÜNİVERSİTESİ

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