Year 2025,
Volume: 20 Issue: 1, 25 - 40, 25.05.2025
İlknur Albayrak
,
Alper Cessur
,
Tunahan Demirci
,
Nilgün Göktürk Baydar
Project Number
TÜBİTAK (The Scientific and Technological Research Council of Turkey) Project number: TOVAG-119O325
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S. Erken and M. E. Özzambak, “Effect of light, temperature and different pretreatments on seed germination of Gentiana boissieri Schott et Kotschy ex Boiss. (Gentianaceae) an endemic to Turkey”, Pakistan Journal of Botany, 49(1), 41-46, 2017.
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T. Demirci, I. Albayrak, H. Deveci, Ö.A. Asci, N.G. Baydar, “Development of effective micropropagation protocols for endangered Gentiana lutea and Gentiana boissieri (endemic to Türkiye)”, Israel Journal of Plant Sciences, 71(3-4), 127-136, 2024.
-
F. Perez-Garcia, F. Varela and M. E. González-Benito, “Morphological and germination response variability in seeds of wild yellow gentian (Gentiana lutea L.) accessions from northwest Spain”, Botany, 90(8), 731-742, 2012.
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M. Fois, A. Cuena-Lombraña, G. Fenu, D. Cogoni and G. Bacchetta, “The reliability of conservation status assessments at regional level: past, present and future perspectives on Gentiana lutea L. ssp. lutea in Sardinia”, Journal for Nature Conservation, 33, 1-9, 2016.
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P. Özdamar Biçer, T. Demirci, Ö. A. Aşcı and N. G. Baydar, “Effects of methyl jasmonate and caffeic acid applications on secondary metabolite production in madder (Rubia tinctorum) root cultures”, Indian Journal of Pharmaceutical Education and Research, 51, 508-512, 2017.
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N. Göktürk Baydar, Z. Babalık, F. Hallaç Türk and E. S. Çetin, “Phenolic composition and antioxidant activities of wines and extracts of some grape varieties grown in Turkey”, Journal of Agricultural Sciences, 17, 67-76, 2011.
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I. Citová, M. Ganzera, H. Stuppner and P. Solich, “Determination of gentisin, isogentisin, and amarogentin in Gentiana lutea L. by capillary electrophoresis”, Journal of Separation Science, 31(1), 195-200, 2008.
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Effects of Methyl Jasmonate on Phenolic Acid Production in in vitro Root Cultures of Gentiana lutea and Gentiana boissieri
Year 2025,
Volume: 20 Issue: 1, 25 - 40, 25.05.2025
İlknur Albayrak
,
Alper Cessur
,
Tunahan Demirci
,
Nilgün Göktürk Baydar
Abstract
This research examines the impact of varying durations and concentrations of methyl jasmonate (MeJA) treatments on the phenolic acid production in in vitro roots of Gentiana lutea and Gentiana boissieri. These species are highly valued for their bioactive metabolites in the pharmaceutical and food industries, as well as their ornamental appeal in horticulture. Nevertheless, both species face threats from overharvesting and human-induced pressures. The study utilized root cultures grown in liquid media, followed by MeJA elicitation at concentrations of 0.1, 0.5, and 1.0 mM for durations of 2, 4, 6, 8, and 10 days. Phenolic acids, including gallic acid (GA), chlorogenic acid (CGA), caffeic acid (CA), p-coumaric acid (p-QA), o-coumaric acid (o-QA), ferulic acid (FA), cinnamic acid (CNA), and rosmarinic acid (RA), were quantified in the harvested roots using HPLC. The results revealed that phenolic acid levels were influenced by both MeJA concentration and application duration. The findings highlight that MeJA, when optimally applied, can enhance phenolic acid production in in vitro root cultures of G. lutea and G. boissieri effectively.
Ethical Statement
As the authors of this study, we declare that we do not have any ethics committee approval and/or informed consent statement. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Supporting Institution
TÜBİTAK TOVAG-119O325
Project Number
TÜBİTAK (The Scientific and Technological Research Council of Turkey) Project number: TOVAG-119O325
Thanks
This study was funded by TÜBİTAK (The Scientific and Technological Research Council of Turkey) under Project No: TOVAG-119O325
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T., Demirci, Ö. Aras Ascı and N. Göktürk Baydar, “Influence of salicylic acid and L-phenylalanine on the accumulation of anthraquinone and phenolic compounds in adventitious root cultures of madder (Rubia tinctorum L.)”, Plant Cell, Tissue and Organ Culture (PCTOC), 144(2), 313-324, 2021.
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H. Deveci, T. Demirci and N. G. Baydar, “Effect of culture time on secondary metabolite production from MeJA-stimulated in vitro roots of Hyoscyamus niger L.”, International Journal of Secondary Metabolite, 9(2), 178-193, 2022.
-
M. Rahmati, P. Golkar and M. Tarkesh, “Effects of methyl jasmonate elicitation on the carvone and limonene contents, phenolic compounds and antioxidant activity in caraway (Carum carvi L.) callus cultures”, Natural Product Research, 1-6, 2023.
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A. Khojasteh, M.H. Mirjalili, J. Palazon, R. Eibl and R.M. Cusido, “Methyl jasmonate enhanced production of rosmarinic acid in cell cultures of Satureja khuzistanica in a bioreactor”, Engineering in Life Sciences, 16(8):740-749, 2016.
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N. Göktürk Baydar, Z. Babalık, F. Hallaç Türk and E. S. Çetin, “Phenolic composition and antioxidant activities of wines and extracts of some grape varieties grown in Turkey”, Journal of Agricultural Sciences, 17, 67-76, 2011.
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