Investigation Biocatalysts of Immobilized Enzyme on New Supports with Ferri and Ferro Nuclei

Abstract

Ferri and ferro coordination polymers in sphere structure were synthesized. Scanning Electron Microscopy (SEM) Energy Dispersive X-Ray Spectroscopy (EDX), Gel Permeation Chromatography (GPC), elemental analysis, and Fourier Transform Infrared Spectroscopy (FT-IR) were performed for chemical and structural characterization of the coordination polymers. Glucose oxidase (GOD) enzyme immobilized to compare of kinetic parameters deal with glucano-1,5 lacton formation. Analyses results illustrate that structures coordination of ions Fe2+ and Fe3+ are different to the same support. It was seen that 2 mol of Fe2+ ion ((PS-N-([Fe(CN)4L]K3)2) was bound per unit structure while 1 mol of Fe3+ ion (PS-N-([Fe(CN)2L]K)) is attaching. Km values of were found as 15.32 and 10.93 for (PS-N-Fe2+)-GOD and (PS-N-Fe3+)-GOD, respectively. Km value for (PS-N-Fe3+)-GOD was found to be 0.5 times higher, possible reason of such a case is the larger reduction potential of Fe3+. As the charge on the coordination structure increased, the enzyme's affinity for the substrate increased. After 20 repeated measurements, GOD immobilized on (PS-N-Fe3+) polymer retained 45.47% activity, while GOD immobilized on (PS-N-Fe2+) polymer retained 57.86% activity.

Keywords

• Ferricyanide
• Ferrocyanide
• Enzyme immobilization
• Coordination polymers
• Glucose oxidase
• Sem-EDX

Ferri ve Ferro çekirdekli Yeni Destekler Üzerinde İmmobilize edilen Enzimin Biyokatalizör Özelliğinin Araştırılması

Abstract

Küre yapısındaki ferri ve ferro koordinasyon polimerleri sentezlendi. Koordinasyon polimerlerinin kimyasal ve yapısal karakterizasyonunda Taramalı Elektron Mikroskopisi (SEM), Enerji Dağılımlı X-Işını Spektroskopisi (EDX), Jel Geçirgenlik Kromatografisi (GPC), elemental analiz ve Fourier Dönüşümlü Kızılötesi Spektroskopisi (FT-IR) kullanılmıştır. Glukano-1,5 lakton oluşumu ile ilgili kinetik parametrelerin karşılaştırılmak için glukoz oksidaz (GOD) enzimi koordinasyon polimerlerine immobilize edilmiştir. Analiz sonuçları, Fe2+ ve Fe3+ iyonlarının aynı desteğe farklı yapılarda koordinasyon gösterdiğini ortaya koymuştur. Birim yapı başına 2 mol Fe2+ iyonu ((PS-N-([Fe(CN)4L]K3)2) bağlanırken, 1 mol Fe3+ iyonunun (PS-N-([Fe(CN)2L]K)) bağlandığı görülmüştür. Km değerleri (PS-N-Fe2+)-GOD ve (PS-N-Fe3+)-GOD için sırasıyla 15,32 ve 10,93 olarak bulunmuştur. (PS-N-Fe3+)-GOD için Km değeri 0,5 kat daha yüksek bulunmuştur, böyle bir durumun olası nedeni Fe3+'ün daha büyük indirgenme potansiyelidir. Tekrarlanan 20 ölçümden sonra, (PS-N-Fe3+) polimeri üzerine immobilize edilen GOD'un %45,47 aktivitesi korunurken, (PS-N-Fe2+) polimeri üzerine immobilize edilen GOD'un %57,86 aktivitesi korunmuştur.

Keywords

• Ferrosiyanür
• Ferrisiyanür
• Enzim İmmobilizasyonu
• Koordinasyon Polimerleri
• Glukoz oksidaz
• SEM-EDS

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