The Effects of Exogenous Application of Ascorbate and Glutathione on Antioxidant System in Cultivated Cicer arietinum and Wild Type C. reticulatum under Drought Stress

Abstract

The roles of ascorbate and glutathione as key antioxidant molecules involves in environmental stress responses have already been well indicated. We conducted experiments in order to study the effects of exogenous ascorbate (ASC) and glutathione (GSH) treatments on antioxidant enzyme activities and ASC and GSH levels of cultivated and wild type chickpea plants under drought stress. We determined that ASC and GSH accumulation, antioxidant enzyme activities increased due to drought stress, except for CAT activity, in both species. ASC treatment increased ASC level and APX activity in C. arietinum under drought stress, except high concentration of ASC treatment. Antioxidant treatments increased antioxidant accumulation of C. reticulatum under drought stress. Antioxidant treatments did not lead to significantly changes in GR activity, in both species. However, exogenous ASC and GSH via eliminating of reactive oxygen species decreased SOD, CAT and APX activities in C. reticulatum. The results of present study indicate that ASC and GSH may contribute to the improvement of tolerance against drought stress in chickpea. Also, drought tolerant chickpea C. reticulatum showed a better protection mechanism against oxidative damage than the sensitive chickpea C. arietinum

Keywords

• Drought stress
• ascorbate
• glutathione
• antioxidants
• chickpea

Kuraklığa Maruz Bırakılan Kültür Nohut Cicer arietinum ve Yabani Nohut C. reticulatum’un Antioksidan Sistemi Üzerine Dışsal Askorbat ve Glutatyon Uygulamalarının Etkisi

Abstract

Askorbat ve glutatyonun çevresel streste anahtar moleküller oldukları iyi bilinmektedir. Kültür nohut Cicer arietinum ve yabani Cicer reticulatum’un antioksidan enzimler, askorbat (ASC) ve glutatyon (GSH) birikimi üzerine kuraklık, ASC ve GSH’ın etkisini çalışmak üzere bir deney hazırlandı. Her iki türde kuraklığa bağlı olarak ASC ve GSH birikiminin ve antioksidan enzim aktivitelerinin katalaz (CAT) aktivitesi hariç arttığını belirledik. ASC uygulaması kuraklık stresi altındaki C. arietinum’da ASC seviyesini ve askorbat peroksidaz (APX) aktivitesini (askorbatın yüksek konsantrasyonu hariç) arttırdı. C. reticulatum’da kuraklık stresi altında antioksidan uygulamaları antioksidan içeriklerini arttırdı. Antioksidan uygulamaları her iki türde de, GR aktivitesinde önemli bir değişikliğe yol açmamıştır. Bununla birlikte, her iki türde ASC ve GSH uygulamaları reaktif oksijen türlerini azaltarak superoxide dismutase (SOD), katalaz (CAT) ve askorbat peroksidaz (APX) aktivitelerini azalttı. Çalışmamız, yapraklara ASC ve GSH uygulamalarının kuraklık stresinin zararlı etkisini azalttığı ve nohutun kuraklığa direnci arttığı hipotezini desteklemektedir. Ayrıca sonuçlarımızdan yola çıkarak C. reticulatum türünün C. arietinum türüne göre oksidatif stres altında daha iyi bir koruma mekanizmasına sahip olduğunu söyleyebiliriz.

Keywords

• Kuraklık stresi
• askorbat
• glutatyon
• antioksidan
• nohut

References

1. Aebi, H.E., Bergmayer, J., Grabl, M. 1983. Catalase. In: Verlag Chemie (Ed) Methods of enzymatic analysis, pp. 273-286. Weinheim.
2. Asada, K. 1999. The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. Annual Review of Plant Physiology and Plant Molecular Biology, 50, 601-639.
3. Aqıl, K., Afmad, I., Z. Mehmood. 2006. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turkish Journal of Biology, 30, 177-183.
4. Basu, S., Aryadeep, R., Progya, P.S., Dibyendu, N.S. 2010. Differential antioxidative responses of indica rice cultivars to drought stress. Journal of Plant Growth Regulation, 60, 51–59.
5. Beyer, W.F., Fridovich, I. 1987. Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions. Analytical Biochemistry, 161, 559-566.
6. Blokhina, O., Virolainen, E., Faferstedt, K.V. 2003. Antioxidants, Oxidative Damage and Oxygen Deprivation Stress. Annals of Botany, 91, 179- 194.
7. Bonnet, M., Camares, O., Veisserie, P. 2000. Effects of zinc and influence of Acremonium lolii on growth parameters, chlorophyll a florescence and antioxidant enzyme activities of ryegrass (Lolium perene L. Cv Apollo). Journal Experimental Botany, 51, 945-953.
8. Carlberg, I., Mannervik, B. 1985. Glutathione Reductase. Method in Enzymology, 113, 484- 490.

9. Çevik, S., Yıldızlı, A., Yandım, G., Göksu, H. Gültekin, M,S,, Güzel Değer, A., Çelik, A., Şimşek Kuş, N., Unyayar. S., (2014). Some synthetic cyclitol derivatives alleviate the effect of water deficit in cultivated and wild-type chickpea species. Journal of Plant Physiology, 171: 807-816.
10. Dolatabadian, A., Sanavy, A.S.M.M., Chasmi, N.A. 2008. The effects of foliar application of ascorbic acid (Vitamin C) on antioxidant enzymes activities, lipid peroxidation and proline accumulation of Canola (Brassica napus L.) under conditions of salt stress. Journal of Agronomy and Crop Science, 194, 206-213.
11. Dolatabadian, A., Jouneghani, S.R. 2009. Impact of Exogenous Ascorbic Acid on Antioxidant Activity and Some Physiological Traits of Common Bean Subjected to Salinity Stres. Notulae Botanicae Horti-Agrobotanici Cluj- Napoca, 37(2), 165-172.
12. Fazeli F., Ghorbanli, M., Niknam, V. 2007. Effect of drought on biomass, protein content, lipid peroxidation and antioxidant enzymes in two sesame cultivars. Biologia Plantarum, 51, 98- 103.
13. Günes, A., İnal, A., Adak, M.S., Bağcı, E.G., Çiçek, N., Eraslan, F. 2008. Effect of drought stress implemented at pre- or post-anthesis stage on some physiological parameters as screening criteria in Chickpea cultivars. Russian Journal of Plant Physiology, 55, 59-67.
14. Hartree, E.F. 1972. Determination of protein: A modification of Lowry method that gives a linear photometric response. Analytical Biochemistry, 48, 422-427.
15. Hawrylak, B., Szymanska, M. 2004. Selebium as a sulphydrylic group inductor in plants. Cellular & Molecular Biology Letters, 9, 329-336.
16. Kalefetoğlu, T., Ekmekçi, Y. 2005. The effects of drought on plants and tolerance mechanisms. Gazi University Journal of Science, 18, 723-740.
17. Morán, J.F., Becana, M, Iturbe I., S. Frechilla, Klucas, R.V., Aparicio, P. 1994. Drought induces oxidative stress in pea plants. Planta, 194, 346- 352.
18. Nayyar. N., Singh, S., Kaur, S., Kumar, S., Upadhyaya, H. 2006. Differential sensitivity of Macrocarpa and Microcarpa types of Chickpea (Cicer arietinum L.) to water stress: Association of contrasting stress response with oxidative injury. Journal of Integrative Plant Biology, 48, 1318-1329.
19. Noctor, G., Foyer, C.H. 1998. Ascorbate and Glutathione: Keeping Active Oxygen Under Control. Annual Review of Plant Physiology and Plant Moleculer Biology, 49, 249-279.
20. Sairam R.K., Deshmukh, P.S., Saxena, D.C. 1998. Role of antioxidant system in wheat genotypes tolerance to water stress. Biologia Plantarum, 41, 387-394.
21. Schmieden, U., Wild, A. 1994. Changes in levels of α- tocopherol and ascorbate in spruce needles at three low mountain sites exposed to Mg+2 deficiency and ozone, Z. Naturforsch 49, 171– 180.
22. Selote, D.S., Khanna-Chopra, R. 2004. Drought- induced spikelet sterility is associated with an inefficient antioxidant defense in rice panicles. Physiologia Plantarum 121, 462-471.
23. Singh, K.B. 1997. Chickpea (Cicer arietinum L.). Field Crops Research, 53,161-170.
24. Unyayar, S., Keleş, Y., Çekiç, F.Ö. 2005. The antioxidative response of two tomato species with different drought tolerances as a result of drought and cadmium stress combinations. Plant Soil Environment 51, 57-64.
25. Vanaker, H., Carver, T.L., Foyer, C.H. 1998. Pathogen- induced changes in the antioxidant status of the apoplast in barley leaves. Plant Physiology, 117, 1103–1114.
26. Wang, C.Q., Xu, H.J., Liu, T. 2011. Effect of
27. selenium on ascorbate-glutathione
28. metabolism during PEG-induced water
29. deficit in Trifolium repens. Journal of Plant
30. Growth Regulation. 30, 436–44.