Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors

Dilek Odacı Demırkol; Caglar Ozdemır; Roberto Pılloton; Suna Tımur

doi:10.19113/sdufbed.06618

Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors

Abstract

Here, a novel enzymatic biosensor was developed using multiwalled carbon nanotube including screen printed electrodes (MWCNT-SPE). Pyranose oxidase (PyOx) was immobilized on the electrode surface by way of gelatin membrane and then cross-linked using glutaraldehyde. Glucose was detected at -0.7 V (vs. Ag/AgCl) by watching consumed oxygen in enzymatic reaction after addition substrate. After optimization of pH and enzyme loading, the linearity was found in the range of 0.1–1.0 mM of glucose. After that, the effect of MCNT on the current was tested. Also the enzymatic biosensor including glucose oxidase instead of pyranose oxidase was prepared and the biosensor response followed for glucose. Furthermore, this system was tested for glucose analysis in soft drinks.

Keywords

Pyranose oxidase,Multiwalled carbon nanotube; Screen printed electrode; Glucose analysis; Gelatin

References

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Details

Primary Language

Turkish

Subjects

-

Journal Section

-

Authors

Dilek Odacı Demırkol

Caglar Ozdemır This is me

Roberto Pılloton This is me

Suna Tımur

Publication Date

March 27, 2017

Submission Date

December 11, 2016

Acceptance Date

-

Published in Issue

Year 2017 Volume: 21 Number: 1

DOI

https://doi.org/10.19113/sdufbed.06618

IZ

https://izlik.org/JA52LS28LG

Cite

RIS / Bibtex

APA

Odacı Demırkol, D., Ozdemır, C., Pılloton, R., & Tımur, S. (2017). Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 21(1), 286-291. https://doi.org/10.19113/sdufbed.06618

AMA

1.Odacı Demırkol D, Ozdemır C, Pılloton R, Tımur S. Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors. J. Nat. Appl. Sci. 2017;21(1):286-291. doi:10.19113/sdufbed.06618

Chicago

Odacı Demırkol, Dilek, Caglar Ozdemır, Roberto Pılloton, and Suna Tımur. 2017. “Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 21 (1): 286-91. https://doi.org/10.19113/sdufbed.06618.

EndNote

Odacı Demırkol D, Ozdemır C, Pılloton R, Tımur S (April 1, 2017) Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 21 1 286–291.

IEEE

[1]D. Odacı Demırkol, C. Ozdemır, R. Pılloton, and S. Tımur, “Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors”, J. Nat. Appl. Sci., vol. 21, no. 1, pp. 286–291, Apr. 2017, doi: 10.19113/sdufbed.06618.

ISNAD

Odacı Demırkol, Dilek - Ozdemır, Caglar - Pılloton, Roberto - Tımur, Suna. “Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 21/1 (April 1, 2017): 286-291. https://doi.org/10.19113/sdufbed.06618.

JAMA

1.Odacı Demırkol D, Ozdemır C, Pılloton R, Tımur S. Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors. J. Nat. Appl. Sci. 2017;21:286–291.

MLA

Odacı Demırkol, Dilek, et al. “Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 21, no. 1, Apr. 2017, pp. 286-91, doi:10.19113/sdufbed.06618.

Vancouver

1.Dilek Odacı Demırkol, Caglar Ozdemır, Roberto Pılloton, Suna Tımur. Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors. J. Nat. Appl. Sci. 2017 Apr. 1;21(1):286-91. doi:10.19113/sdufbed.06618

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