Analysis of Autoimmune Diseases-Human Gut Microbiota Association Using Transcriptome Data and Omic Tools

Abstract

Recent advances in omic analyses and the accurate determination of DNA sequencing of genomes of human-related microbial communities have helped increase the knowledge of the function and structure of human microbiota in diseased and healthy states. However there is still not much work done in this regard. In this study,  three important autoimmune diseases, Psoriasis, Rheumatoid Arthritis and Atopic Dermatitis were selected. Firstly the transcriptional regulation for these diseases were integrated with metabolic pathways using omic tools. Secondly the link between human intestinal microbial species and these diseases were revealed. The results have shown that there is a mutual group of microbial species among the selected diseases. These are intensively species of Firmicutes, Deltaproteobacteria, Bacteriodetes and Actinobacteria phyla. Another noteworthy aspect of this study is that the microbial species of the Tenericutes phyla are intensely present in atopic dermatitis and rheumatoid arthritis but not in psoriasis. These results display that human gut microbiota has a role in complex autoimmune diseases. This study showed that not only genes and proteins, but also metabolites, microbiota and pathogen groups should be examined and analyzed in detail in order to comprehend the mechanism of a disease.    

Keywords

• Autoimmunity,Psoriasis,Atopic dermatitis,Rheumatoid arthritis,Metabolite,Human gut microbiota

Transkriptom Verisi ve Omik Araçları Kullanılarak Otoimmün Hastalıklar ile İnsan Bağırsak Mikrobiyotası Arasındaki İlişkinin Analizi

Abstract

İnsan bağırsak mikrobiyotası ile insan hastalıkları arasındaki ilişkinin anlamlandırılabilmesi konusunda yapılan çalışmalar yakın zamanda ivme kazanmıştır. Bunun sebebi insan mikrobiyomunun hastalıklı ve sağlıklı hallerdeki işlevi ve yapısı hakkında daha çok bilgiye sahip olunması ve ilişkili mikrobiyal toplulukların genomlarının DNA diziliminin doğru belirlenmesi şeklinde açıklanabilir. Yine de bu konuda çok fazla çalışma bulunmamaktadır. Mevcut çalışmada üç önemli otoimmün hastalık olan Psoriazis, Romatoid Artrit ve Atopik Dermatit’in, omiks araçları ile önce transkripsiyon regülasyonu metabolik ağa entegre edilmiş daha sonra ise bu hastalıkların insan bağırsak mikrobiyotası ile arasındaki bağlantı ortaya çıkarılmıştır. Elde edilen sonuçlara göre seçilmiş olan hastalıklar ile ilgili ortak mikrobiyal türler mevcuttur. Bunlar yoğun olarak Firmicutes, Deltaproteobacteria, Bacteriodetes ve Actinobacteria filumundaki türlerdir. Bu çalışmada bir başka dikkat çeken husus ise Tenericutes filumunun mikrobiyal türlerinin Atopik dermatit ve Romatoid artritte yoğun olarak görüldüğü fakat Psoriaziste çok fazla çeşitlilik göstermediğidir. Bu sonuçlar insan bağırsak mikrobiyotasının kompleks otoimmün hastalıklarda bir rolünün olduğunu göstermektedir. Yapılan bu çalışma bir hastalığın mekanizmasını anlayabilmek için sadece genler ve proteinler değil bunların yanında metabolitler, mikrobiyota ve patojen grupların da detaylıca incelenip analiz edilmesi gerektiğini ortaya koymaktadır.

Keywords

• Otoimmünite,Psoriazis,Atopik Dermatit,Romatoid Artrit,Metabolit,İnsan Bağırsak Mikrobiyatası

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