Hızlandırılmış Yaşlandırma Testi Sonrasında Aspir (Carthamus tinctorius L.) Tohumlarında Hidrojen Peroksit İçeriği ve Antioksidant Enzim Aktivitelerinin Belirlenmesi

Abstract

Aspir dünyada önemli bir yağlı tohum bitkisidir. Yaşlandırma testleri, tohumların uzun süreli depolanması sırasında meydana gelen hücresel hasarın oluşturulmasını sağlayan testlerdir. Mevcut çalışmada hızlandırılmış yaşlandırma testi (AA) aspir tohumlarında antioksidant enzim aktivitelerinin incelenmesi için uygulanmıştır. Daha önceki çalışmada yaşlanmaya dirençli (Bayer-6 ve Bayer-12) yaşlanmaya hassas (Olas ve Linas) dört aspir genotipi tohum materyali olarak kullanılmıştır. AA, 43 °C 5 farklı zamanda (0, 48, 72, 96 ve 120 saat) gerçekleştirilmiştir. Sonuçlar varyans analizine tabi tutulmuş ve ortalamalar arasındaki önemlilik seviyeleri tespit edilmiştir. Ayrıca hidrojen peroksit (H2O2) içeriği ve katalaz (CAT) ve süperoksit dismutaz (SOD) aktiviteleri arasındaki korelasyon incelenmiştir. H2O2 içeriği, CAT, SOD ve peroksidaz (POD) aktiviteleri kontrol ve AA uygulanmış tohumlarda belirlenmiştir. Genotip, zaman ve genotip x zaman interaksiyonları önemli bulunmuştur. H2O2 içeriği ve SOD aktivitesi AA zamanına bağlı olarak artarken, CAT aktivitesi ise kullanılan tüm genotiplerde ise azalmıştır. POD aktivitesi düzenli bir şekilde artış veya azalış göstermemiş, aktivitedeki değişim genotip ve zamana göre farklılık göstermiştir. H2O2 içeriği ve CAT aktivitesi arasındaki korelasyon tüm genotiplerde önemli ve negatif olarak bulunurken; H2O2 içeriği ve SOD aktivitesi arasında AA uygulanmış tohumlarda pozitif korelasyon bulunmuştur.

Keywords

• Katalaz
• Peroksidaz
• Tohum yaşlanması
• Süperoksit dismutaz

Determination of Hydrogen Peroxide Content and Antioxidant Enzyme Activities in Safflower (Carthamus tinctorius L.) Seeds After Accelerated Aging Test

Abstract

Safflower is an important oleiferous crop species in the world. Aging tests are used to simulate cell damage occurring during the long term storage of seeds. In the present study, accelerated aging (AA) test was employed to investigate response of antioxidant enzymes in safflower. Four genotypes of safflower, previously classified as aging resistant (Bayer-6 and Bayer-12) and sensitive (Olas and Linas) based on AA test, were used as seed materials and AA treatments at 43 °C consisted of 5 different times (0, 48, 72, 96 and 120 h). Variance analysis were used and means were separated according to significance levels, and correlations were calculated between hydrogen peroxide (H2O2) content, superoxide dismutase (SOD) and catalase (CAT) activities. The H2O2 content, CAT, SOD and peroxidase (POD) activities were measured in control and AA treated seeds. Genotype, time and genotype x time interactions were all significant. While H2O2 content and SOD activity increased with AA time, CAT activity decreased in all genotypes throughout the experiment. POD did not show regular increase or decrease, its activity was specific to genotypes and time. Correlations between CAT activity and H2O2 content were significant negative for all genotypes, but between SOD activity and H2O2 content was positively correlated in AA treated seeds.

Keywords

• Catalase
• Peroxidase
• Seed aging
• Superoxide dismutase

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