BibTex RIS Cite

Bakır Asetoarsenit'in İn Vivo Genotoksik Etkilerinin Değerlendirilmesi

Year 2009, Volume: 13 Issue: 2, 132 - 136, 26.02.2014

Hasan Türkez Abdulgani Tatar Fatime Geyikoğlu Başak Togar Mevlüt Keleş

Abstract

Mevcut çalışmada, Sprague-Dawley sıçanlarının kemik iliği hücreleri üzerinde Bakır asetoarsenit (BAA) in genotoksik etkileri araştırıldı. Bu amaçla, sıçanlar rastgele altı gruba ayrıldı ve her bir grupta yedi hayvan yer aldı. Kontrol grubu hariç, BAA dozları (5, 10, 15, 25 ve 30 mgkg-1) deney hayvanlarına beş gün boyunca intraperitonal (i.p) yolla verildi. BAA enjeksiyonlarından sonra sıçanlar anestezi edildi ve bu sürenin sonunda kemik iliği hücreleri alındı. Kromozom aberasyonlarını (KA) tespit etmek için preparatlar Giemsa ile boyandı ve ışık mikroskobu ile incelendi. Bunun yanı sıra, mikroçekirdek (MÇ) frekansı May-Grünwald ve Giemsa ile boyanmış preparatlarda değerlendirildi. Kontrol grubuyla karşılaştırıldığında, BAA ile muameleden sonra yapısal KA sayısı ve MÇ frekansları belirgin bir şekilde arttı. Aynı zamanda, kemik iliği hücrelerinde BAA in genotoksik etkilerinin doza bağlı olarak arttığı tespit edildi. Anahtar Kelimeler: Bakır Asetoarsenit, Kromozom Aberasyonları, Mikroçekirdek, Genotoksisite.

Keywords

Bakır Asetoarsenit Kromozom Aberasyonları Mikroçekirdek Genotoksisite

References

  • Agarwal, K., Sharma, A., Talukder, G. 1990. Clastogenic Effects of Copper Sulphate on the Bone Marrow Chromosomes of Mice in Vivo. Mutation Research, 243(1), 1-6.
  • Andrewes, P., Kitchin, K.T., Wallace, K. 2003. Dimethylarsine and Trimethylarsine are Potent Genotoxins in vitro. Chem Res Toxicol, 16(8), 994-1003.
  • Baldwin, D.R., Marshall, W.J. 1999. Heavy Metal Poisoning and ıts Laboratory Investigation. Ann Clin Biochem, 36, 267-300.
  • Bhunya, S.P., Jena, G.B. 1996. Clastogenic Effects of Copper Sulphate in Chick in vivo Test System. Mutation Research, 367(2), 57-63.
  • Bukowska, B. 2004. Effects of 2,4-D and ıts Metabolite 2,4-dichlorophenol on Antioxidant Enzymes and Level of Glutathione in Guman Erythrocytes. Comparative Biochemistry and Physiology. Toxicology and Pharmacology, 135, 435-441.
  • Chatterjee, A., Das, D., Chakraborti, D. 1993. A Study of Ground Water Contamination by Arsenic in The Residential Area of Behala, Calcutta Due to Industrial Pollution. Environmental Pollution, 80, 57-65.
  • Colognato, R., Coppede, F., Ponti, J., Sabbioni, E., Migliore, L. 2007. Genotoxicity Induced by Arsenic Compounds in Peripheral Human Lymphocytes Analysed by Cytokinesis-Block Micronucleus Assay. Mutagenesis, 22(4), 255-261.
  • D’Angelo, D., Norton, S.A., Loiselle, M.C. 1996. Historical Uses and Fate of Arsenic in Maine. Water Research Institute Completion Report, University of Maine, Orono, Maine.
  • De Roos, A.J., Zahm, S.H., Cantor, K.P., Weisenburger, D.D., Holmes, F.F., Burmeister, L.F., Blair, A. 2003. Integrative Assessment of Multiple Pesticides as Risk Factors for Non-Hodgkin's Lymphoma Among Men. Occup Environ Med, 60(9), 11.
  • Devlet Planlama Teskilatı, (DPT). 2001. Kimya Sanayi Özel htisas Komisyonu Raporu, Tarım laçları Alt Komisyonu Raporu, Ankara.
  • Dulout, F.N., Grillo, C.A., Seoane, A.I., Maderna, C.R., Nilsson,R., Vahter, M., Darroudi, F., Natarajan, A.T. 1996. Chromosomal Aberrations in Peripheral Blood Iymphocytes from Native Andrean Women And Children from Northwestern Argentian Exposed to Arsenic in Drinking Water. Mutation Research, 370 (3-4), 151-158.
  • Ergene, S., Çelik, A., Cavas, T., Kaya, F. 2007. Genotoxic Biomonitoring Study of Population Residing in Pesticide Contaminated Regions in Göksu Delta: Micronucleus, Chromosomal Aberrations and Sister Chromatid Exchanges. Environmental International, 33(7), 877-885.
  • Gebel, T.W. 2001. Genotoxicity of Arsenical Compounds. International Journal of Hygiene and Environmental Health, 203(3), 249-262.
  • Graham-Evans, B., Cohly, H.H., Yu, H., Tchounwou, P.B. 2004. Arsenic-induced Genotoxic and Cytotoxic Effects in Human Keratinocytes, Melanocytes and Dendritic Cells. Int J Environ Res Public Health, 1(2), 83-89.
  • Guillamet, E., Creus, A., Ponti, J., Sabbioni, E., Fortaner, S., Marcos, R. 2004. In Vitro DNA Damage by Arsenic Compounds in A Human Lymphoblastoid Cell Line (TK6) Assessed By The Alkaline Comet Assay. Mutagenesis, 19(2): 129-135.
  • Güler, Ç., Çobanoglu, Z. 1994. Bireyin s ve Çevresel Zararlara Cevabını Degistiren Durumlar. Saglık Bakanlıgı Temel Saglık Hizmetleri Genel Müdürlügü ve Saglık Projesi Genel Koordinatörlügü, Çevre Saglıgı Temel Kaynak Dizisi, No:4, Ankara.
  • Hei, T.K., Filipic, M. 2004. Role of Oxidative Damage in the Genotoxicity of Arsenic. Free Radic Biol Med, 37(5), 574-581.
  • Hooiveld, M., Heederick, D.J.J., Kogevinas, M., Boffetta, P., Needham, L. L., Patterson, D.G., Bas BuenodeMesquita, H. 1998. Second Follow-up of a Dutch Cohort Occupationally Exposed to Phenoxy Herbicides, Chlorophenols, and Contaminants. Am. J. Epidemiol, 147, 891-901.
  • Hunt, C.D., Idso, J.P. 1999. Dietary Boron as a Physiological Regulator of The nflammatory Response: A Review and Current Research Progress. J. Trace Element Experimental Medicine, 12, 221-233.
  • Kessel, M., Liu, S.X., Xu, A., Santella, R., Hei, T.K. 2002. Arsenic Induces Oxidative DNA Damage in Mammalian Cells. Mol. Cell. Biochem, 234, 301– 308.
  • Lee, T.C., Ho, I.C. 1994. Differential Cytotoxic Effects of Arsenic in Human and Animal Cells. Environmental Health Perspect, 102(3), 101-105.
  • Lin, A., Zhang, X., Zhu, Y.G., Zhao, F.J. 2008. ArsenateInduced Toxicity: Effects on Antioxidative Enzymes and DNA Damage in Vicia Faba. Environ Toxicol Chem, 27(2), 413-419.
  • Mahata, J., Basu, A., Ghoshal, S., Sarkar, J.N., Roy, A.K., Poddar, G., Nandy A.K., Banerjee, A., Ray, K. 2003. Chromosomal Aberrations and Sister Chromatid Exchanges in Individuals Exposed to Arsenic Through Drinking Water in West Bengal, India. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 534(1-2), 133-143.
  • Mazumder, D.N., Das Gupta, J., Chakraborty, A.K., Chatterjee, A., Das, D., Chakraborti, D. 1992. Environmental Pollution and Chronic Arsenicosis in South Calcutta, Bull World Health Organ, 70, 481-485.
  • Natarajan, A.T., Obe, G., Hayashi, M. 2008. Chromosomal Aberrations. Mutation Research/ Genetic Toxicology and Environmental Mutagenesis, 657(1), 1-2.
  • Patlolla, A.K., Tchounwou, P.B. 2005. Cytogenetic Evaluation of Arsenic Trioxide Toxicity in Sprague-Dawley Rats. Mutation Research, 587(12), 126-133.
  • Peryea F.J. 1998. Historical Use of Lead Arsenate Insecticides, Resulting Soil Contamination and Implications for Soil Remediation. 16th World Congress of Soil Science, Montpellier, France.
  • Rahman, I., Biswas, S.K., Jimenez, L.A., Torres, M., Jay Forman, H. 2005. Glutathione, Stres Responses, and Redox Signaling in Lung Inflammation. Antioxidant Redox Signaling, 7(1-2), 42-59.
  • Roy, L.D., Mazumdar, M., Giri, S. 2008. Effects of LowDose Radiation and Vitamin C Treatment on Chloroquine-Induced Genotoxicity in Mice. Environmental and Molecular Mutagenesis, 49, 488-495.
  • Rudel, R., Slayton T. M., Beck B. D. 1996. Implications of Arsenic Genotoxicty for Dose Response of Carcinogenic Effects. Regul Toxicol Pharmacol, 23 (2), 87-105.
  • Schmid, W. 1976. The Micronucleus Test for Cytogenetic Analysis. Pp. 31-53. In: Hollaender, A. (editor). Chemical Mutagens: Principles and Methods for Their Detection, Vol. 4, New York.
  • Shimizu, M., Hochadel, J. F., Fulmer, B. A. and Waalkes, M.P. 1998. Effect of Glutathione Depletion and Metallothionein Gene Expression on Arsenicnduced Cytotoxicity and c-myc Expression in vitro. Toxicological Sciences, 45(2), 204-211
  • Shubber, E., Amin N.S., El-Adhami, B.H. 1998. Cytogenetic Effects of Copper-Containing Intrauterine Contraceptive Device (IUCD) on Blood Iymphocytes. Mutation Research, 417(2-3), 57-63.
  • Svistunenko, D.A. 2005. Reaction of Haem Containing Proteins and Enzymes with Hudroperoxides: The Radical View. Biochimica Biophysica Acta, 1707, 127-155.
  • Sanlı, Y. 2002. Veteriner Klinik Toksikoloji. Güngör Matbaacılık, stanbul, 808 pp.
  • Tekbas, Ö.F., Ogur, R. 2008. Arsenik, çme Suları ve Saglık. TAF Preventive Medicine Bulletin, 7, 4.
  • Türkez, H., Topal, A., Geyikoglu, F. 2006. Bakır Asetoarsenitin nsan Eritrositlerinde Antioksidan Enzimler ve Glutatyon Seviyesi Üzerine Etkileri. VI. Ulusal Ekoloji ve Çevre Kongresi, 18-21 Eylül, Diyarbakır, 135-142.
  • Yagmur, F., Hancı, Y.H. 2002. Arsenik. Sürekli Tıp Egitimi Dergisi, 11(7), 250-251.
  • Yüzbasıoglu, D., Celik, M., Yılmaz, S., Unal, F., Aksoy, H. 2006. Clastogenicity of The Fungicide Afugan in Cultured Human lymphocytes. Mutation Research, 604, 53.

Year 2009, Volume: 13 Issue: 2, 132 - 136, 26.02.2014

Hasan Türkez Abdulgani Tatar Fatime Geyikoğlu Başak Togar Mevlüt Keleş

Abstract

References

  • Agarwal, K., Sharma, A., Talukder, G. 1990. Clastogenic Effects of Copper Sulphate on the Bone Marrow Chromosomes of Mice in Vivo. Mutation Research, 243(1), 1-6.
  • Andrewes, P., Kitchin, K.T., Wallace, K. 2003. Dimethylarsine and Trimethylarsine are Potent Genotoxins in vitro. Chem Res Toxicol, 16(8), 994-1003.
  • Baldwin, D.R., Marshall, W.J. 1999. Heavy Metal Poisoning and ıts Laboratory Investigation. Ann Clin Biochem, 36, 267-300.
  • Bhunya, S.P., Jena, G.B. 1996. Clastogenic Effects of Copper Sulphate in Chick in vivo Test System. Mutation Research, 367(2), 57-63.
  • Bukowska, B. 2004. Effects of 2,4-D and ıts Metabolite 2,4-dichlorophenol on Antioxidant Enzymes and Level of Glutathione in Guman Erythrocytes. Comparative Biochemistry and Physiology. Toxicology and Pharmacology, 135, 435-441.
  • Chatterjee, A., Das, D., Chakraborti, D. 1993. A Study of Ground Water Contamination by Arsenic in The Residential Area of Behala, Calcutta Due to Industrial Pollution. Environmental Pollution, 80, 57-65.
  • Colognato, R., Coppede, F., Ponti, J., Sabbioni, E., Migliore, L. 2007. Genotoxicity Induced by Arsenic Compounds in Peripheral Human Lymphocytes Analysed by Cytokinesis-Block Micronucleus Assay. Mutagenesis, 22(4), 255-261.
  • D’Angelo, D., Norton, S.A., Loiselle, M.C. 1996. Historical Uses and Fate of Arsenic in Maine. Water Research Institute Completion Report, University of Maine, Orono, Maine.
  • De Roos, A.J., Zahm, S.H., Cantor, K.P., Weisenburger, D.D., Holmes, F.F., Burmeister, L.F., Blair, A. 2003. Integrative Assessment of Multiple Pesticides as Risk Factors for Non-Hodgkin's Lymphoma Among Men. Occup Environ Med, 60(9), 11.
  • Devlet Planlama Teskilatı, (DPT). 2001. Kimya Sanayi Özel htisas Komisyonu Raporu, Tarım laçları Alt Komisyonu Raporu, Ankara.
  • Dulout, F.N., Grillo, C.A., Seoane, A.I., Maderna, C.R., Nilsson,R., Vahter, M., Darroudi, F., Natarajan, A.T. 1996. Chromosomal Aberrations in Peripheral Blood Iymphocytes from Native Andrean Women And Children from Northwestern Argentian Exposed to Arsenic in Drinking Water. Mutation Research, 370 (3-4), 151-158.
  • Ergene, S., Çelik, A., Cavas, T., Kaya, F. 2007. Genotoxic Biomonitoring Study of Population Residing in Pesticide Contaminated Regions in Göksu Delta: Micronucleus, Chromosomal Aberrations and Sister Chromatid Exchanges. Environmental International, 33(7), 877-885.
  • Gebel, T.W. 2001. Genotoxicity of Arsenical Compounds. International Journal of Hygiene and Environmental Health, 203(3), 249-262.
  • Graham-Evans, B., Cohly, H.H., Yu, H., Tchounwou, P.B. 2004. Arsenic-induced Genotoxic and Cytotoxic Effects in Human Keratinocytes, Melanocytes and Dendritic Cells. Int J Environ Res Public Health, 1(2), 83-89.
  • Guillamet, E., Creus, A., Ponti, J., Sabbioni, E., Fortaner, S., Marcos, R. 2004. In Vitro DNA Damage by Arsenic Compounds in A Human Lymphoblastoid Cell Line (TK6) Assessed By The Alkaline Comet Assay. Mutagenesis, 19(2): 129-135.
  • Güler, Ç., Çobanoglu, Z. 1994. Bireyin s ve Çevresel Zararlara Cevabını Degistiren Durumlar. Saglık Bakanlıgı Temel Saglık Hizmetleri Genel Müdürlügü ve Saglık Projesi Genel Koordinatörlügü, Çevre Saglıgı Temel Kaynak Dizisi, No:4, Ankara.
  • Hei, T.K., Filipic, M. 2004. Role of Oxidative Damage in the Genotoxicity of Arsenic. Free Radic Biol Med, 37(5), 574-581.
  • Hooiveld, M., Heederick, D.J.J., Kogevinas, M., Boffetta, P., Needham, L. L., Patterson, D.G., Bas BuenodeMesquita, H. 1998. Second Follow-up of a Dutch Cohort Occupationally Exposed to Phenoxy Herbicides, Chlorophenols, and Contaminants. Am. J. Epidemiol, 147, 891-901.
  • Hunt, C.D., Idso, J.P. 1999. Dietary Boron as a Physiological Regulator of The nflammatory Response: A Review and Current Research Progress. J. Trace Element Experimental Medicine, 12, 221-233.
  • Kessel, M., Liu, S.X., Xu, A., Santella, R., Hei, T.K. 2002. Arsenic Induces Oxidative DNA Damage in Mammalian Cells. Mol. Cell. Biochem, 234, 301– 308.
  • Lee, T.C., Ho, I.C. 1994. Differential Cytotoxic Effects of Arsenic in Human and Animal Cells. Environmental Health Perspect, 102(3), 101-105.
  • Lin, A., Zhang, X., Zhu, Y.G., Zhao, F.J. 2008. ArsenateInduced Toxicity: Effects on Antioxidative Enzymes and DNA Damage in Vicia Faba. Environ Toxicol Chem, 27(2), 413-419.
  • Mahata, J., Basu, A., Ghoshal, S., Sarkar, J.N., Roy, A.K., Poddar, G., Nandy A.K., Banerjee, A., Ray, K. 2003. Chromosomal Aberrations and Sister Chromatid Exchanges in Individuals Exposed to Arsenic Through Drinking Water in West Bengal, India. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 534(1-2), 133-143.
  • Mazumder, D.N., Das Gupta, J., Chakraborty, A.K., Chatterjee, A., Das, D., Chakraborti, D. 1992. Environmental Pollution and Chronic Arsenicosis in South Calcutta, Bull World Health Organ, 70, 481-485.
  • Natarajan, A.T., Obe, G., Hayashi, M. 2008. Chromosomal Aberrations. Mutation Research/ Genetic Toxicology and Environmental Mutagenesis, 657(1), 1-2.
  • Patlolla, A.K., Tchounwou, P.B. 2005. Cytogenetic Evaluation of Arsenic Trioxide Toxicity in Sprague-Dawley Rats. Mutation Research, 587(12), 126-133.
  • Peryea F.J. 1998. Historical Use of Lead Arsenate Insecticides, Resulting Soil Contamination and Implications for Soil Remediation. 16th World Congress of Soil Science, Montpellier, France.
  • Rahman, I., Biswas, S.K., Jimenez, L.A., Torres, M., Jay Forman, H. 2005. Glutathione, Stres Responses, and Redox Signaling in Lung Inflammation. Antioxidant Redox Signaling, 7(1-2), 42-59.
  • Roy, L.D., Mazumdar, M., Giri, S. 2008. Effects of LowDose Radiation and Vitamin C Treatment on Chloroquine-Induced Genotoxicity in Mice. Environmental and Molecular Mutagenesis, 49, 488-495.
  • Rudel, R., Slayton T. M., Beck B. D. 1996. Implications of Arsenic Genotoxicty for Dose Response of Carcinogenic Effects. Regul Toxicol Pharmacol, 23 (2), 87-105.
  • Schmid, W. 1976. The Micronucleus Test for Cytogenetic Analysis. Pp. 31-53. In: Hollaender, A. (editor). Chemical Mutagens: Principles and Methods for Their Detection, Vol. 4, New York.
  • Shimizu, M., Hochadel, J. F., Fulmer, B. A. and Waalkes, M.P. 1998. Effect of Glutathione Depletion and Metallothionein Gene Expression on Arsenicnduced Cytotoxicity and c-myc Expression in vitro. Toxicological Sciences, 45(2), 204-211
  • Shubber, E., Amin N.S., El-Adhami, B.H. 1998. Cytogenetic Effects of Copper-Containing Intrauterine Contraceptive Device (IUCD) on Blood Iymphocytes. Mutation Research, 417(2-3), 57-63.
  • Svistunenko, D.A. 2005. Reaction of Haem Containing Proteins and Enzymes with Hudroperoxides: The Radical View. Biochimica Biophysica Acta, 1707, 127-155.
  • Sanlı, Y. 2002. Veteriner Klinik Toksikoloji. Güngör Matbaacılık, stanbul, 808 pp.
  • Tekbas, Ö.F., Ogur, R. 2008. Arsenik, çme Suları ve Saglık. TAF Preventive Medicine Bulletin, 7, 4.
  • Türkez, H., Topal, A., Geyikoglu, F. 2006. Bakır Asetoarsenitin nsan Eritrositlerinde Antioksidan Enzimler ve Glutatyon Seviyesi Üzerine Etkileri. VI. Ulusal Ekoloji ve Çevre Kongresi, 18-21 Eylül, Diyarbakır, 135-142.
  • Yagmur, F., Hancı, Y.H. 2002. Arsenik. Sürekli Tıp Egitimi Dergisi, 11(7), 250-251.
  • Yüzbasıoglu, D., Celik, M., Yılmaz, S., Unal, F., Aksoy, H. 2006. Clastogenicity of The Fungicide Afugan in Cultured Human lymphocytes. Mutation Research, 604, 53.
There are 39 citations in total.

Details

Primary Language English
Journal Section TEMEL BİLİMLER
Authors

Hasan Türkez This is me

Abdulgani Tatar This is me

Fatime Geyikoğlu This is me

Başak Togar This is me

Mevlüt Keleş This is me

Publication Date February 26, 2014
Published in Issue Year 2009 Volume: 13 Issue: 2

Cite

APA Türkez, H., Tatar, A., Geyikoğlu, F., Togar, B., et al. (2014). Bakır Asetoarsenit’in İn Vivo Genotoksik Etkilerinin Değerlendirilmesi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 13(2), 132-136. https://doi.org/10.19113/sdufbed.58846
AMA Türkez H, Tatar A, Geyikoğlu F, Togar B, Keleş M. Bakır Asetoarsenit’in İn Vivo Genotoksik Etkilerinin Değerlendirilmesi. J. Nat. Appl. Sci. February 2014;13(2):132-136. doi:10.19113/sdufbed.58846
Chicago Türkez, Hasan, Abdulgani Tatar, Fatime Geyikoğlu, Başak Togar, and Mevlüt Keleş. “Bakır Asetoarsenit’in İn Vivo Genotoksik Etkilerinin Değerlendirilmesi”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 13, no. 2 (February 2014): 132-36. https://doi.org/10.19113/sdufbed.58846.
EndNote Türkez H, Tatar A, Geyikoğlu F, Togar B, Keleş M (February 1, 2014) Bakır Asetoarsenit’in İn Vivo Genotoksik Etkilerinin Değerlendirilmesi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 13 2 132–136.
IEEE H. Türkez, A. Tatar, F. Geyikoğlu, B. Togar, and M. Keleş, “Bakır Asetoarsenit’in İn Vivo Genotoksik Etkilerinin Değerlendirilmesi”, J. Nat. Appl. Sci., vol. 13, no. 2, pp. 132–136, 2014, doi: 10.19113/sdufbed.58846.
ISNAD Türkez, Hasan et al. “Bakır Asetoarsenit’in İn Vivo Genotoksik Etkilerinin Değerlendirilmesi”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 13/2 (February 2014), 132-136. https://doi.org/10.19113/sdufbed.58846.
JAMA Türkez H, Tatar A, Geyikoğlu F, Togar B, Keleş M. Bakır Asetoarsenit’in İn Vivo Genotoksik Etkilerinin Değerlendirilmesi. J. Nat. Appl. Sci. 2014;13:132–136.
MLA Türkez, Hasan et al. “Bakır Asetoarsenit’in İn Vivo Genotoksik Etkilerinin Değerlendirilmesi”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 13, no. 2, 2014, pp. 132-6, doi:10.19113/sdufbed.58846.
Vancouver Türkez H, Tatar A, Geyikoğlu F, Togar B, Keleş M. Bakır Asetoarsenit’in İn Vivo Genotoksik Etkilerinin Değerlendirilmesi. J. Nat. Appl. Sci. 2014;13(2):132-6.

e-ISSN :1308-6529
Linking ISSN (ISSN-L): 1300-7688

All published articles in the journal can be accessed free of charge and are open access under the Creative Commons CC BY-NC (Attribution-NonCommercial) license. All authors and other journal users are deemed to have accepted this situation. Click here to access detailed information about the CC BY-NC license.