Demir ve Alüminyumun Doğal Sularda UV Spektrofotometrik Tayini İçin Yöntem Geliştirilmesi ve Elde Edilen Verilerin Kemometrik Kalibrasyon Yöntemleriyle Değerlendirilmesi

Year 2014, Volume: 18 Issue: 3, 52 - 59, 07.01.2015

A. Hakan Aktaş Bengisu Mutlutürk

Abstract

Bu çalışmada, kemometrik kalibrasyon yöntemlerinden temel bileşen analizi yöntemi (PCA), temel bileşen regresyonu yöntemi (PCR) ve kısmi en küçük kareler yöntemi (PLS), doğal sularda bulunan Fe³âº ve Al³âº'ün miktar tayininde başarı ile uygulanmıştır. UV- Görünür Alan Spektroskopisinden elde edilen veriler kemometrik olarak değerlendirilmiştir. Uygulamada iki farklı ligand chrome azurol S (CAS) ve pyrocatechol violet (PCV) denenmiş ve daha kararlı sonuçlar elde edildiğinden CAS ile çalışmalara devam edilmiştir. Fe³âº ve Al³âº CAS ile komplekslerinin spektrofotometrik yöntemlerle uygun parametreleri belirlenmiş (ligand miktarı, pH, bekleme süreleri, metal derişimi ve absorbans arasındaki ilişki ve yabancı iyon etkisi) ve optimum koşullar sağlanmıştır. Optimum koşullar sağlandıktan sonra Fe³âº ve Al³âº içeren doğal su örneğinin spektrofotometrik tayinleri gerçekleştirilmiş ve elde edilen veriler kemometrik olarak değerlendirilmiştir.

Keywords

PCA, PCR, PLS, UV-Görünür Alan, Spektroskopisi, Kemometri, CAS, PCV

UV Spectrophotometric Method Development for the Determination of Iron and Aluminum in Natural Waters and Evaluation of the data Obtained from the Calibration of Chemometric Methods

Abstract

In this study, principal component analysis method (PCA), principal component regression method (PCR), partial least squares method (PLS) of chemometry calibration methods, are successfully realized on quantitation of Fe³⁺ and Al³⁺ present at natural water. The data gathered from UV- Visible Spectroscopy are evaluated chemometrically. In the application two distinctive ligand chrome azurol S (CAS) and pyrocatechol violet (PCV) are tested and studies are proceeded with CAS due to more stable results that are obtained. Optimum parameters (ligand amount, pH, waiting period, relation between metal concentration and absorbance, foreign ion effect) and conditions of Fe³⁺ and Al³⁺ CAS and their complexes are determined with spectrophotometric methods. After the optimum conditions are provided, spectrophotometric determinations of natural water including Fe³⁺ and Al³⁺ are realized and obtained data are evaluated as chemometry

Keywords

PCA, PCR, PLS, UV-Visible, Spectrophotometer, Chemometry, CAS, PCV

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