The Effects of Exogenous Application of Ascorbate and Glutathione on Antioxidant System in Cultivated Cicer arietinum and Wild Type C. reticulatum under Drought Stress

Year 2015, Volume: 19 Issue: 1, 91 - 97, 21.03.2015

Sertan Çevik , Serpil Ünyayar

Abstract

The roles of ascorbate and glutathione as key antioxidant molecules involves in environmental stress responses have already been well indicated. We conducted experiments in order to study the effects of exogenous ascorbate (ASC) and glutathione (GSH) treatments on antioxidant enzyme activities and ASC and GSH levels of cultivated and wild type chickpea plants under drought stress. We determined that ASC and GSH accumulation, antioxidant enzyme activities increased due to drought stress, except for CAT activity, in both species. ASC treatment increased ASC level and APX activity in C. arietinum under drought stress, except high concentration of ASC treatment. Antioxidant treatments increased antioxidant accumulation of C. reticulatum under drought stress. Antioxidant treatments did not lead to significantly changes in GR activity, in both species. However, exogenous ASC and GSH via eliminating of reactive oxygen species decreased SOD, CAT and APX activities in C. reticulatum. The results of present study indicate that ASC and GSH may contribute to the improvement of tolerance against drought stress in chickpea. Also, drought tolerant chickpea C. reticulatum showed a better protection mechanism against oxidative damage than the sensitive chickpea C. arietinum

Keywords

Drought stress, ascorbate, glutathione, antioxidants, chickpea

Kuraklığa Maruz Bırakılan Kültür Nohut Cicer arietinum ve Yabani Nohut C. reticulatum’un Antioksidan Sistemi Üzerine Dışsal Askorbat ve Glutatyon Uygulamalarının Etkisi

Abstract

Askorbat ve glutatyonun çevresel streste anahtar moleküller oldukları iyi bilinmektedir. Kültür nohut Cicer arietinum ve yabani Cicer reticulatum’un antioksidan enzimler, askorbat (ASC) ve glutatyon (GSH) birikimi üzerine kuraklık, ASC ve GSH’ın etkisini çalışmak üzere bir deney hazırlandı. Her iki türde kuraklığa bağlı olarak ASC ve GSH birikiminin ve antioksidan enzim aktivitelerinin katalaz (CAT) aktivitesi hariç arttığını belirledik. ASC uygulaması kuraklık stresi altındaki C. arietinum’da ASC seviyesini ve askorbat peroksidaz (APX) aktivitesini (askorbatın yüksek konsantrasyonu hariç) arttırdı. C. reticulatum’da kuraklık stresi altında antioksidan uygulamaları antioksidan içeriklerini arttırdı. Antioksidan uygulamaları her iki türde de, GR aktivitesinde önemli bir değişikliğe yol açmamıştır. Bununla birlikte, her iki türde ASC ve GSH uygulamaları reaktif oksijen türlerini azaltarak superoxide dismutase (SOD), katalaz (CAT) ve askorbat peroksidaz (APX) aktivitelerini azalttı. Çalışmamız, yapraklara ASC ve GSH uygulamalarının kuraklık stresinin zararlı etkisini azalttığı ve nohutun kuraklığa direnci arttığı hipotezini desteklemektedir. Ayrıca sonuçlarımızdan yola çıkarak C. reticulatum türünün C. arietinum türüne göre oksidatif stres altında daha iyi bir koruma mekanizmasına sahip olduğunu söyleyebiliriz.

Keywords

Kuraklık stresi, askorbat, glutatyon, antioksidan, nohut

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