Comparison of Volatile Compounds using Headspace Solid-phase Microextraction (HSSPME) and Conventional Hydro-distillation Method in Oil-bearing Rose (Rosa damascena Mill.)

Year 2016, Volume: 20 Issue: 1, 27 - 36, 15.04.2016

Hasan Baydar , Sabri Erbaş

https://doi.org/10.19113/sdufbed.14857

Cited By: 2

Abstract

Oil-bearing rose (Rosa damascena Mill.) is one of the most strongly scented rose species, having characteristic floral scent compounds, which is used in the fragrance and cosmetic industries. In this research, floral scent compounds of the fresh oil-bearing rose flower divided into sections such as sepal, petal, stamen and pistil were detected by using headspace solid phase microextraction (HS-SPME) combined with gas chromatography/mass spectrometry (GC-MS). And also scent compounds of essential oils distilled from fresh rose flowers by using a conventional Clevenger type hydro-distillation apparatus were detected by GC-MS to make comparison with HS-SPME results. The findings indicated that the HSSPME was sensitive and effective for the analysis of floral scent compounds of oilbearing rose flower that’s each section had different scent composition. The scent compositions of the rose oil obtained by hydro-distillation were different than floral scent composition of the fresh rose flowers. This result pointed out that hydro-distillation caused the loss of some floral scent compounds of oil-bearing rose flower. Other important findings were that phenyl ethyl alcohol, the main scent compound of the fresh rose flower, was found especially in the pink flower petals, and methyl eugenol, an undesirable compound of rose oil, was found especially in the flower stamens.

Keywords

Oil-bearing rose, Rosa damascena, HS-SPME, GC-MS, Hydro-distillation, Essential oil

Yağ Gülü (Rosa damascena Mill.)’nde Tepe Boşluğu Katı Faz Mikro Ekstraksiyonu (HS-SPME) ve Konvansiyonel Su Distilasyonu Yöntemleri ile Elde Edilen Uçucu Bileşenlerin Karşılaştırılması

Abstract

Yağ gülü (Rosa damascena Mill.), kendine özgü doğal koku bileşenleri nedeniyle parfüm ve kozmetik endüstrisinde değerlendirilen en önemli kokulu gül türüdür. Bu araştırmada, taze yağ gülü çiçeklerinin sepal (çanak yaprak), petal (taç yaprak), stamen (erkek organ) ve pistil (dişi organ) olarak ayrılan kısımlarının uçucu bileşenleri gaz kromatografisi/kütle spektrometresi (GC-MS) ile kombine edilmiş Tepe Boşluğu-Katı Faz Mikro Ekstraksiyon (HS-SPME) tekniği ile tespit edilmiştir. Ayrıca taze yağ gülü çiçekleri konvansiyonel hidro-Clevenger su distilasyonu yöntemi ile damıtılmış, elde edilen uçucu yağların bileşenleri GC-MS ile belirlendikten sonra HS-SPME sonuçları ile karşılaştırılmıştır. HS-SPME yönteminin yağ gülünde uçucu bileşenleri analizinde oldukça hassas ve etkili olduğu anlaşılmış, bir yağ gülü çiçeğinin farklı kısımların farklı koku bileşenlerine sahip olduğu belirlenmiştir. Ayrıca su distilasyonu ile elde edilen gül yağının uçucu yağ bileşenlerinin çiçeğin doğal koku bileşenlerinden farklı olduğu ve distilasyon sürecinde önemli bazı bileşenlerde değişimler ve kayıplar olduğu tespit edilmiştir. Taze gül çiçeğinin en önemli bileşeni olan feniletil alkol çiçeğin en fazla pembe renkli petallerinde (taç yapraklarında), gül yağında varlığı istenmeyen metil öjenol ise çiçeğin en fazla stamenlerinde (erkek organlarında) bulunduğu belirlenmiştir.

Keywords

Yağ gülü, Rosa damascena, HS-SPME, GC-MS, Su distilasyonu, Uçucu yağ

References

• Lawrence, B.M. 1991. Progress in Essential Oils: Rose Oil and Extracts. Perfumer & Flavorist, 16, 43-77.
• Baydar, H. 2006. Oil-Bearing Rose (Rosa damascena Mill.) Cultivation and Rose Oil Industry in Turkey. Euro Cosmetics, 14, 13-17.
• Kovacheva, N., Rusanov, K., Atanassov, I. 2010. Industrial Cultivation of Oil Bearing Rose and Rose Oil Production in Bulgaria during 21st Century Directions and Challenges. Biotechnology & Biotechnology Equipment, 24 (2), 1793-1789.
• Aydınlı, M., Tutaş, M. 2003. Production of Rose Absolute from Rose Concrete. Flavour And Fragrance Journal, 18(1), 26–31.
• Kürkçüoğlu, M., Başer, K. H.C. 2003. Studies on Turkish Rose Concrete, Absolute and Hydrosol. Chemistry of Natural Compounds, 39(5), 457-464.
• Aycı, F., Aydınlı, M., Bozdemir, O.A., Tutaş, M. 2005. Gas Chromotographic Investigation of Rose Concrete, Absolute and Solid Residue. Flavour and Fragrance Journal, 20, 481-486.
• Baydar, H., Kazaz, S. 2013. Yağ Gülü & Isparta Gülcülüğü. Gülbirlik Yayınları. No:1. Isparta.
• Dudareva, N., Pichersky, E. 2000. Biochemical and Molecular Genetic Aspects of Floral Scents. Plant Physiology, 122, 627-633.
• Baydar, H., Schulz, H., Kruger, H., Erbaş. S., Kineci, S. 2008. Influences of Fermentation Time, Hydro-distillation Time and Fractions on Essential Oil Composition of Damask Rose (Rosa damascena Mill.). Journal of Essential Oil Bearing Plants, 11 (3), 224-232.
• Anaç, O. 1984. Gas Chromatographic Analysis on Turkish Rose Oil, Absulute and Concrete. Perfumer & Flavorist, 9, 1-14.
• Başer, K.H.C. 1992. Turkish Rose Oil. Perfumer & Flavorist, 17, 45-52.
• Bayrak, A., Akgül, A. 1994. Volatile Oil Composition of Turkish Rose (Rosa damascena). Journal of the Science of Food and Agriculture, 64, 441-448.
• Başer, K.H.C., Kürkçüoğlu, M., Özek, T. 2003. Turkish Rose Oil: Recent Results. Perfumer & Flavorist, 28(2), 34-42.
• Cherry-Martin, M., Jullien, F., Heizmann, P., Baudino, S. 2007. Fragrance Heritability in Hybrid Tea Roses. Scientia Horticulturae, 113, 177-181.
• Oka, N., Ohishi, H., Hatona, T., Hornberger, M., Sakata, K., Watanabe, N. 1999. Aroma Evolation During Flower Opening in Rosa damascena Mill. Zeitschrift für Naturforschung, 54, 889-895.
• Spiller, M., Berger, R.G., Debener., T., 2010. Genetic Dissection of Scent Metabolic Profiles in Diploid Rose Populations. Theoretical and Applied Genetics, 120, 1461-1471
• Garnero, J., Buil, P. 1976. Evolution of the Composition of the Rose Essential Oils and Concrete During the Production Campaign. Riv. Ital Ess Prof Peante Offic Aromic Sap., Aerosol, 58, 537-540.
• Babu, K.G., Singh, B.S., Joshi, P.J., Singh, V. 2002. Essential Oil Composition of Damask Rose (Rosa damascena Mill.) Distilled under Different Pressures and Temperatures. Flavour and Fragrance Journal, 17, 136-140.
• Jirovetz, L., Buchbauer, G., Stoyanova, A., Balinova, A., Guangjiun, Z., Xihan, M. 2005. Solid Phase Microextraction/Gas Chromatographic and Olfactory Analysis of the Scent and Fixative Properties of The Essential Oil of Rosa damascena L. from China. Flavour and Fragrance Journal, 20, 7-12.
• Héthelyi, É.B., Szarka, S., Lemberkovics, É., Szőke, É. 2010. SPME-GC/MS Identification of Aroma Compounds in Rose Flowers. Acta Agronomica Hungarica, 58 (3), 283-287.
• Dobreva, A. 2013. Dynamics of the Headspace Chemical Components of Rosa damascena Mill. Flowers. Journal of Essential Oil Bearing Plants, 16, 404-411.
• European Pharmacopoeia 1975. Maissonneuve Sainteruffine, 3, 68.
• David, F., De Clercq, C., Sandra, P. 2006.GC/MS/MS Analysis of β-damascenone in Rose Oil. Varian GC/MS App., Note 52.
• Baydar, H., Erbaş, S., Kineci, S., Kazaz, S. 2007. Yağ Gülü (Rosa damascena Mill.) Damıtma Suyuna Katılan Tween-20'nin Taze ve Fermente Olmuş Çiçeklerin Gül Yağı Verimi ve Kalitesi Üzerine Etkisi. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 2(1), 15-20.
• Eikani, M.H., Golmohammad, F., Rowshanzamir, S., Mirza, M. 2005. Recovery of Water-Soluble Constituents of Rose Oil Using Simultaneous Distillation–Extraction. Flavour And Fragrance Journal, 20(6), 555–558.
• Kineci, S., Baydar, H., Cengiz, M. 2007. Production of Essential Oil using Adsorbent Resins from Waste Water that Remained While Rose Oil Production. 38th International Symposium on Essential Oils. Book of Abstracts, No: SL-29, September 9-12, 2007. Graz, Austria.
• Mihailova, J., Atanasouva, R., Balinova-Tsvetkova, A. 1977. Direct Gas Chromatographs of the Essential Oil in Separate Flower Parts of the Flower of Kazanlik Rose. In Proc. 7th Int. Cong. Ess. Oils. Kyoto. Japan., 219-221.
• Özkan, G., Sağdıç, O., Baydar, N.G., Baydar, H. 2004. Antioxidant and Antibacterial Activities of Rosa damascena flower extracts. Food Science and Technology, 10(4), 277-281.
• Baydar, H., Erbaş, S., Kaya, Y. 2011. Ayçiçeğinde Feromon Olarak Gül Suyunun Kullanılması Üzerine Bir Araştırma. 9. Tarla Bitkileri Kongresi, 12-14 Eylül, Bursa, 2, 964-997.
• Baydar, H., Baydar Göktürk, N. 2005. The Effects of Harvest Date, Fermentation Duration and Tween 20 Treatment on Essential Oil Content and Composition of Industrial Oil Rose (Rosa damascena Mill.). Industrial Crops and Products, 21, 251-255.
• Baydar, H., Kazaz, S., Erbaş, S. 2013. Yağ Gülünde (Rosa damascena Mill.) Morfogenetik, Ontogenetik ve Diurnal Varyabiliteler. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 8(1), 1-11.
• MESC, 2000. Methyl Eugenol Steering Committee Meeting. International Workshop on P-Alkoxyallylbenzene Derivatives - Methyl Eugenol and Estragole. May 1-2, 2000, Virginia.
• Harris, B. 2002. Methyl Eugenol-The Current Bete Noire of Aromatherapy. International Journal of Aromatherapy, 12(4), 193-201.
• Rusanov, K., Kovacheva, N., Rusanova, M., Atanassov, I. 2012. Reducing Methyl Eugenol Content in Rosa damascena Mill. Rose Oil by Changing the Traditional Rose Flower Harvesting Practices. The journal European Food Research and Technology, 234, 921–926.
• NTP 2000. Toxicology and Carcinogenesis Studies of Methyl eugenol (CAS No. 93–15–2) in F344/N Rats and B6C3F1 Mice. National Toxicology Program Technical Report Series, 491, 1–412.
• SCCNFP 2000. Opinion Concerning Methyl eugenol Adopted By The SCCNFP During The 14th Plenary Meeting Of 24 October 2000. Executive Summary. Scientific Committee On Cosmetic Products And Non-Food Products Intended For Consumers. http://ec.europa.eu/health/scientific_committees/consumer_safety (Erişim Tarihi 25.09.2013).
• Health Canada (2010). List of Prohibited And Restricted Cosmetic Ingredients (The Cosmetic Ingredient Hotlist). http://www.hc-sc.gc.ca/hotlist-liste_2010-eng.pdf (Erişim Tarihi 20.10.2013).
• Göktürk Baydar, N., Baydar, H. 2013. Phenolic Compounds, Antiradical Activity and Antioxidant Capacity of Oil-Bearing Rose (Rosa damascena Mill.). Industrial Crops and Products, 41, 375-380.