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Gelişme Dönemi ve Kurutma Şeklinin Çörtük Otu (Echinophora tenuifolia subsp. sibthorpiana Tutin)’nun Uçucu Yağ Oranı ve Bileşenleri Üzerine Etkilerinin Belirlenmesi

Year 2016, Volume: 20 Issue: 1, 43 - 49, 15.04.2016
https://doi.org/10.19113/sdufbed.59206

Abstract

Araştırma, Isparta florasında yaygın olarak yetişen çörtük otu (Echinophora tenuifolia subsp. sibthorpiana Tutin) bitkisinin gelişme dönemi boyunca uçucu yağ oran ve bileşenlerinin belirlenmesi amacıyla 2012 yılında yürütülmüştür. Bitkiler fide, sapa kalkma, tam çiçeklenme ve tohum bağlama dönemlerinde toplanmıştır. Uçucu yağ analizleri hem taze olarak hem de gölge ve güneşte kurutulduktan sonra yapılmıştır. Örneklerdeki uçucu yağ miktarları clavenger tipi hidro-distilasyon cihazında, uçucu yağ bileşenleri ise GC/MS cihazında belirlenmiştir. Bitkilerin uçucu yağ oranlarının gelişme dönemlerine göre önemli varyasyonlar gösterdiği, taze örneklerde fide döneminde % 0.76 olan uçucu yağ oranının tohum bağlama döneminde % 1.06’ya yükseldiği belirlenmiştir. Gölgede kurutulan örneklerin uçucu yağ oranları taze ve güneşte kurutulan örneklere göre daha yüksek bulunmuştur. Uçucu yağ bileşenleri bitki gelişme dönemine ve kurutma yöntemine bağlı olarak önemli değişimler göstermiştir. Bitkinin tüm gelişme dönemlerinde de uçucu yağın yaklaşık 20 farklı bileşenden oluştuğu ve ana bileşenlerin αphellandrene (% 47.43 - 66.39) ve methyl eugenol (% 21.29 – 38.72) olduğu belirlenmiştir. Hem taze hem de kurutulan örneklerde gelişme dönemi ilerledikçe methtl eugenol azalırken, α-phellandrene artış göstermiştir. Çalışmada, çörtük otu bitkisinde gelişme dönemi ve kurutma yönteminin uçucu yağ oranı ve bileşenleri üzerine önemli etkiler gösterdiği ve bitkinin kullanım amacına bağlı olarak hasat zamanı ve kurutma yönteminin dikkate alınması gerektiği sonucuna varılmıştır. 

References

  • Mabberley, D. J. 1997. The Plant Book (2nd ed.), Cambridge University Press, Cambridge.
  • Akgul, A., Chialva, F. 1989. Constituents of the Essential Oil of Echinophora tenuifolia L. subsp. sibthorpiana (Guss.) Tutin from Turkey, Flavour and Fragrance Journal, 4, 67.
  • Tutin, T. G. 1968. Echinophora L.in Flora Europaea, T.G. Tutin, V.H. Heywood, N.A. Burges, D.M. Moore, D.H. Valentine, S.M. Waltersand D.A. Webb, Cambridge University Press, 2, 324.
  • Baytop, T. 1984. Therapy with Medicinal Plants in Turkey (Pastand Present), Istanbul Univ. Publ. No. 3255, Istanbul.
  • Değirmencioğlu, N., Göçmen, D., Dağdelen, A., Dağdelen, F. 2005. Influence of Tarhana Herb (Echinophora sibthorpiana) on Fermentation of Tarhana Turkish Traditional Fermented Food. Food Technology and Biotechnology, 43, 2175–2179.
  • Telci İ., Hışıl, Y. 2008. Essential Oil Composition of the spice plant Echinophora tenuifolia L. subsp. sibthorpiana Tutin from Turkey. Acta Botanica Hungarica, 45(1), 163-167.
  • Akşit, H., Demrbas, İ., Telci, İ., Tarimcilar, G. 2013. Chemical Diversity in Essential Oil Composition of Mentha longifolia (L.) hudson subsp. typhoides (Briq.) Harley var. typhoides from Turkey. The Journal of Essential Oil Research, 25(5), 430–437.
  • Alwani, S., El Omari, K., Sukarieh, İ., Soufi, H., Jama, C., Zreika, S. 2014. Effect of Organ Type, Drying Methods and Extraction Time on Yield and Essential Oil Components of Micromeria barbata L. before Flowering. International Journal of Research in Medical and Health Sciences, 4(5), 43-47.
  • Hamrouni-Sellami, I., Wannes, W. A., Bettaieb, I., Berrima, S., Chahed, T., Marzouk, B., Limam, F. 2011. Qualitative and Quantitative Changes in the Essential Oil of Laurus nobilis L. leaves as Affected by Different Drying Methods. Food Chemistry, 126, 691–769.
  • Asekun, O. T., Grierson, D. S., Afolayan, A. J. 2007. Effects of drying Methods on the Quality and Quantity of the Essential Oil of Mentha longifolia L. subsp. capensis. Food Chemistry, 107, 995–998.
  • Ahmadi, K., Sefidkon, F., Osareh, M.H. 2008. Effect of Drying Methods on Quantity and Quality of Essential Oil Three Genotype of Rosa damascene Mill. Iranian Journal of Medicinal and Aromatic Plants, 24(2), 162-176.
  • Diana, L., Ronicely, R., Evandro, M., Evan, V., Antonio, P. 2008. Influence of Drying Air Temperature on the Essential Oil Content from Melaleuca alternifolia Cheel. International Conference of Agricultural Engineering, August 31 -September 4, Brazil.
  • Okoh, O. O., Sadimenko, A. P., Asekun, O. T., Afolayan, A. J. 2008. The Effects of Drying on the Chemical Components of Essential Oils of Calendula officinalis L. African Journal of Biotechnology, 7(10), 1500-1502.
  • Ronicely, R., Evandro, M., Lauril, R., Luiz, B., Jose, M. 2008. Effect of Drying Air Temperature upon the Essential oil Content from Guaco. International Conference of Agricultural Engineering, August 31 -September 4, Brazil.
  • Başer, K. H. C., Erdemgil, F. Z., Özek, T. 1994. Essential oil of Echinophora tenuifolia L. subsp. sibthorpiana (Guss.) Tutin. Journal of Essential Oil Research, 6, 399-400.
  • Baser, K. H. C., Özek, T., Demircakmak, B., Bicakci, A., Malyer, H. 1996. Essential Oil of Echinophora chrysantha Freyn et Sint. Journal of Essential Oil Research, 8(4), 4333-434.
  • Baser, K. H. C., Kurkcuoglu, M., Malyer, H., Bicakci, A. 1998. Essential Oils of Six Echinophora species from Turkey. Journal of Essential Oil Research, 10(3), 345-351.
  • Ahmadi, L., Mirza, M., Khorram, M. T. 2001. Essntial oil of Echinophora cinerea (Boiss.) Hedge and Lamond from İran. Journal of Essential Oil Research, 13, 82-83.
  • Özcan, M., Akgül, A. 2005. Essential Oil Composition of Turkish Pickling Herb (Echinophora tenuifolia L. subsp. sibthorpiana (Guss.) Tutin). Acta Botanica Hungarica, 45(1/2), 163-167.
  • Stein, S. E. 1990. National Institute of Standards and Technology (NIST) Mass Spectral Database and Software, Version 3.02, Juen USA.
  • SAS Institute. 2009. INC SAS/STAT Users’ Guide release 7.0, Cary, NC, USA.
  • Braga, N. P., Cremasco, M. A., Valle, R. C. C. R. 2005. The effects of Fixed-Bed Drying on the Yield and Composition of Essential Oil from Long Pepper (Piper hispidinervium C. DC) Leaves. Brazilian Journal of Chemical Engineering, 22, 257–262.
  • Moyler, D. A. 1994. Spices Recent Advances. in G. Charalambous Ed., Spices, Herbs and Edible Fungi, Amsterdam: Elsevier, 1-70 pp.
  • Khalid A. K., Hu, W., Cai, W. 2008. The Effects of Harvesting and Different Drying Methods on the Essential Oil Composition of Lemon Balm (Melissa officinalis L.). Journal of Essential Oil Bearing Plants, 11(4), 342 - 349.
  • Blanco, M. C. S. G., Ming, L. C., Marques, M. O. M., Bovi, O.A. 2002. Drying Temperature Effects in Peppermint Essential Oil content and Composition. Acta Horticulturae, 569, 95.
  • Şekeroglu, N., Özgüven, M., Erden, U. 2007. Effects of the Drying Temperature on Essential Oil Content of Bay Leaf (Laurus nobilis L.) Harvested at Different Times. Acta Horticulturae, 756, 315.
  • Agah, M., Najafian, S. 2012. Essential Oil Content and Composition of Lippa citriodora as Affected by Drying Method before Flowering Stages. European Journal of Experimental Biology, 2 (5), 1771-1777.
  • Chalchat, J. C., Özcan, M. M., Dağdelen, A., Akgül, A. 2007. Variability of Essential Oil Composition of Echinophora tenuifolia subsp. sibthorpiana Tutin by Harvest Location and Year and Oil Storage. Chemistry of Natural Compounds, 43(2), 225-227.
  • Gokbulut, İ., Bilenler, T., Karabulut, İ. 2013. Determination of Chemical Composition, Total Phenolic, Antimicrobial, and Antioxidant Activities of Echinophora tenuifolia Essential Oil. International Journal of Food Properties, 16, 1442–1451.
  • Ghani, A., Saharkhiz, M. J., Hassanzadeh, M., Msaada, K. 2009. Changes in the Essential Oil Content and Chemical Compositions of Echinophora platyloba DC. during Three Different Growth and Developmental Stages. Journal of Essential Oil Bearing Plants, 12(2), 162-171.
  • Rahimmalek, M., Goli, S. A. H. 2013. Evaluation of Six Drying Treatments with Respect to Essential oil Yield, Composition and Color Characteristics of Thymys daenensis subsp. daenensis. Celak Leaves. Industrial Crops and Products, 42, 613–619.
  • Fraternale, D., Ricci, D., Epifano, F., Curini, M. 2004. Composition and Antifungal Activity of two Essential Oils of Hyssop (Hyssopus officinalis L.). Journal of Essential Oil Research, 16, 617–622.
  • Góra, J., Lis, A. 2002. Olejek Hyzopowy. Aromaterapia, 1(27), 29–32.
  • Wolski T., Baj T. 2006. Hyzop Lekarski (Hyssopus officinalis L.) Aromatyczna Roślina lecznicza. Aromaterapia, 4(46), 10–18.
  • Wesołowska, A., Jadczak, D., Grzeszczuk, M. 2010. Essential Oil Composition of (Hyssopus officinalis L.) Cultivated in North-Western Poland. Herba Polonica, 56(1), 57–65.
  • Özgüven, M., Tansı, S. 1998. Drug Yield and Essential Oil of Thymus vulgaris L. as in Influenced by Ecological and on to Genetical Variation. Turkish Journal of Agriculture and Forestry, 22, 537–542.
  • Mirjalili, M. H., Salehi, P., Sonboli, A., Mohammadi, M. 2006. Essential oil Variation of Salvia officinalis during its Phenological Cycle. Chemistry of Natural Compounds, 42(1), 19-23.
  • Sefidkon, F., Abbasi, Kh., Bakhshi Khaniki Gh. 2006. Influence of Drying and Extraction Method on Yield and Chemical Composition of the Essential Oil of Saturea hortensis. Food Chemistry, 99(1), 19-23.
  • Kizil, S., Toncer, O., Ipek, A., Arslan, N., Saglam, S., Khawar, K. M. 2008. Blooming Stages of Turkish Hyssop (Hyssopus officinalis L.) Affect Essential Oil Composition. Acta Agriculturae Scandinavica, Section B, Soil and Plant Science, 58(3), 273–279.
  • Nurzyńska-Wierdak, R., Dzida, K. 2009. Influence of Plant Density and Term of Harvest on Yield and Chemical Composition of Sweet Marjoram (Origanum majorana L.). Acta Scientiarum Polonorum Hortorum Cultus, 8(1), 51–61.
  • Kaškonienie,V., Kaškonas, P., Maruška, A., Ragažinskiene, O. 2011. Chemical Composition and Chemometric Analysis of Essentials Oils Variation of Bidens tripatita L. during Vegetation Stages. Acta Physiologiae Plantarum, 33, 2377–2385.
  • Georgiou, C., Tzakou, O., Koutsaviti, A., Bazos, I. 2010. Chemical Composition of Echinophora tenuifolia subsp. sibthorpiana Essential Oil from Greece. Records of Natural Products 4(3), 167-170.
  • Özcan, M., Akgül, A., Chalchat, J. C. 2002. Composition of the Essential Oil of Echinophora tenuifolia L. ssp. sibthorpiana (Guss.) Tutin from Turkey. Journal of Essential Oil Research, 14(1), 23-24.

Growth Stage and Drying Methods Affect Essential Oil Content and Composition of Pickling Herb (Echinophora tenuifolia subsp. sibthorpiana Tutin)

Year 2016, Volume: 20 Issue: 1, 43 - 49, 15.04.2016
https://doi.org/10.19113/sdufbed.59206

Abstract

The present research was conducted during 2012 in order to determine the essential oil content and composition of Echinophora tenuifolia subsp. sibthorpiana Tutin. Plants were collected during rosette, vegetative growth, full flowering and fruit-ripening stages. Oil was extracted using Clavenger hydrodistillation apparatus from either fresh, shade dried or sun dried samples. Oil composition was determined with a GC/MS. Oil content of samples showed significant variation during the vegetative stages of development. Oil contents of fresh samples were found to be 0.76% at seedling stage whereas oil content has risen to 1.06% at seed set. The shade-dried samples had higher oil contents than the fresh and sun dried samples. The oil composition of pickling herb changed with drying method and growth stage. Throughout the growth stage of the plant, the oil was composed of 21 components and the main components were found to be αphellandrene (47.43 - 66.39%) and methyl eugenol (21.29 – 38.72%). While methyl eugenol content decreased during vegetation period for both fresh and dried samples, α-phellandrene level increased. Attention should be given to the collection time and drying method of pickling herb for different uses since vegetative stage and drying method influence oil content and composition.

References

  • Mabberley, D. J. 1997. The Plant Book (2nd ed.), Cambridge University Press, Cambridge.
  • Akgul, A., Chialva, F. 1989. Constituents of the Essential Oil of Echinophora tenuifolia L. subsp. sibthorpiana (Guss.) Tutin from Turkey, Flavour and Fragrance Journal, 4, 67.
  • Tutin, T. G. 1968. Echinophora L.in Flora Europaea, T.G. Tutin, V.H. Heywood, N.A. Burges, D.M. Moore, D.H. Valentine, S.M. Waltersand D.A. Webb, Cambridge University Press, 2, 324.
  • Baytop, T. 1984. Therapy with Medicinal Plants in Turkey (Pastand Present), Istanbul Univ. Publ. No. 3255, Istanbul.
  • Değirmencioğlu, N., Göçmen, D., Dağdelen, A., Dağdelen, F. 2005. Influence of Tarhana Herb (Echinophora sibthorpiana) on Fermentation of Tarhana Turkish Traditional Fermented Food. Food Technology and Biotechnology, 43, 2175–2179.
  • Telci İ., Hışıl, Y. 2008. Essential Oil Composition of the spice plant Echinophora tenuifolia L. subsp. sibthorpiana Tutin from Turkey. Acta Botanica Hungarica, 45(1), 163-167.
  • Akşit, H., Demrbas, İ., Telci, İ., Tarimcilar, G. 2013. Chemical Diversity in Essential Oil Composition of Mentha longifolia (L.) hudson subsp. typhoides (Briq.) Harley var. typhoides from Turkey. The Journal of Essential Oil Research, 25(5), 430–437.
  • Alwani, S., El Omari, K., Sukarieh, İ., Soufi, H., Jama, C., Zreika, S. 2014. Effect of Organ Type, Drying Methods and Extraction Time on Yield and Essential Oil Components of Micromeria barbata L. before Flowering. International Journal of Research in Medical and Health Sciences, 4(5), 43-47.
  • Hamrouni-Sellami, I., Wannes, W. A., Bettaieb, I., Berrima, S., Chahed, T., Marzouk, B., Limam, F. 2011. Qualitative and Quantitative Changes in the Essential Oil of Laurus nobilis L. leaves as Affected by Different Drying Methods. Food Chemistry, 126, 691–769.
  • Asekun, O. T., Grierson, D. S., Afolayan, A. J. 2007. Effects of drying Methods on the Quality and Quantity of the Essential Oil of Mentha longifolia L. subsp. capensis. Food Chemistry, 107, 995–998.
  • Ahmadi, K., Sefidkon, F., Osareh, M.H. 2008. Effect of Drying Methods on Quantity and Quality of Essential Oil Three Genotype of Rosa damascene Mill. Iranian Journal of Medicinal and Aromatic Plants, 24(2), 162-176.
  • Diana, L., Ronicely, R., Evandro, M., Evan, V., Antonio, P. 2008. Influence of Drying Air Temperature on the Essential Oil Content from Melaleuca alternifolia Cheel. International Conference of Agricultural Engineering, August 31 -September 4, Brazil.
  • Okoh, O. O., Sadimenko, A. P., Asekun, O. T., Afolayan, A. J. 2008. The Effects of Drying on the Chemical Components of Essential Oils of Calendula officinalis L. African Journal of Biotechnology, 7(10), 1500-1502.
  • Ronicely, R., Evandro, M., Lauril, R., Luiz, B., Jose, M. 2008. Effect of Drying Air Temperature upon the Essential oil Content from Guaco. International Conference of Agricultural Engineering, August 31 -September 4, Brazil.
  • Başer, K. H. C., Erdemgil, F. Z., Özek, T. 1994. Essential oil of Echinophora tenuifolia L. subsp. sibthorpiana (Guss.) Tutin. Journal of Essential Oil Research, 6, 399-400.
  • Baser, K. H. C., Özek, T., Demircakmak, B., Bicakci, A., Malyer, H. 1996. Essential Oil of Echinophora chrysantha Freyn et Sint. Journal of Essential Oil Research, 8(4), 4333-434.
  • Baser, K. H. C., Kurkcuoglu, M., Malyer, H., Bicakci, A. 1998. Essential Oils of Six Echinophora species from Turkey. Journal of Essential Oil Research, 10(3), 345-351.
  • Ahmadi, L., Mirza, M., Khorram, M. T. 2001. Essntial oil of Echinophora cinerea (Boiss.) Hedge and Lamond from İran. Journal of Essential Oil Research, 13, 82-83.
  • Özcan, M., Akgül, A. 2005. Essential Oil Composition of Turkish Pickling Herb (Echinophora tenuifolia L. subsp. sibthorpiana (Guss.) Tutin). Acta Botanica Hungarica, 45(1/2), 163-167.
  • Stein, S. E. 1990. National Institute of Standards and Technology (NIST) Mass Spectral Database and Software, Version 3.02, Juen USA.
  • SAS Institute. 2009. INC SAS/STAT Users’ Guide release 7.0, Cary, NC, USA.
  • Braga, N. P., Cremasco, M. A., Valle, R. C. C. R. 2005. The effects of Fixed-Bed Drying on the Yield and Composition of Essential Oil from Long Pepper (Piper hispidinervium C. DC) Leaves. Brazilian Journal of Chemical Engineering, 22, 257–262.
  • Moyler, D. A. 1994. Spices Recent Advances. in G. Charalambous Ed., Spices, Herbs and Edible Fungi, Amsterdam: Elsevier, 1-70 pp.
  • Khalid A. K., Hu, W., Cai, W. 2008. The Effects of Harvesting and Different Drying Methods on the Essential Oil Composition of Lemon Balm (Melissa officinalis L.). Journal of Essential Oil Bearing Plants, 11(4), 342 - 349.
  • Blanco, M. C. S. G., Ming, L. C., Marques, M. O. M., Bovi, O.A. 2002. Drying Temperature Effects in Peppermint Essential Oil content and Composition. Acta Horticulturae, 569, 95.
  • Şekeroglu, N., Özgüven, M., Erden, U. 2007. Effects of the Drying Temperature on Essential Oil Content of Bay Leaf (Laurus nobilis L.) Harvested at Different Times. Acta Horticulturae, 756, 315.
  • Agah, M., Najafian, S. 2012. Essential Oil Content and Composition of Lippa citriodora as Affected by Drying Method before Flowering Stages. European Journal of Experimental Biology, 2 (5), 1771-1777.
  • Chalchat, J. C., Özcan, M. M., Dağdelen, A., Akgül, A. 2007. Variability of Essential Oil Composition of Echinophora tenuifolia subsp. sibthorpiana Tutin by Harvest Location and Year and Oil Storage. Chemistry of Natural Compounds, 43(2), 225-227.
  • Gokbulut, İ., Bilenler, T., Karabulut, İ. 2013. Determination of Chemical Composition, Total Phenolic, Antimicrobial, and Antioxidant Activities of Echinophora tenuifolia Essential Oil. International Journal of Food Properties, 16, 1442–1451.
  • Ghani, A., Saharkhiz, M. J., Hassanzadeh, M., Msaada, K. 2009. Changes in the Essential Oil Content and Chemical Compositions of Echinophora platyloba DC. during Three Different Growth and Developmental Stages. Journal of Essential Oil Bearing Plants, 12(2), 162-171.
  • Rahimmalek, M., Goli, S. A. H. 2013. Evaluation of Six Drying Treatments with Respect to Essential oil Yield, Composition and Color Characteristics of Thymys daenensis subsp. daenensis. Celak Leaves. Industrial Crops and Products, 42, 613–619.
  • Fraternale, D., Ricci, D., Epifano, F., Curini, M. 2004. Composition and Antifungal Activity of two Essential Oils of Hyssop (Hyssopus officinalis L.). Journal of Essential Oil Research, 16, 617–622.
  • Góra, J., Lis, A. 2002. Olejek Hyzopowy. Aromaterapia, 1(27), 29–32.
  • Wolski T., Baj T. 2006. Hyzop Lekarski (Hyssopus officinalis L.) Aromatyczna Roślina lecznicza. Aromaterapia, 4(46), 10–18.
  • Wesołowska, A., Jadczak, D., Grzeszczuk, M. 2010. Essential Oil Composition of (Hyssopus officinalis L.) Cultivated in North-Western Poland. Herba Polonica, 56(1), 57–65.
  • Özgüven, M., Tansı, S. 1998. Drug Yield and Essential Oil of Thymus vulgaris L. as in Influenced by Ecological and on to Genetical Variation. Turkish Journal of Agriculture and Forestry, 22, 537–542.
  • Mirjalili, M. H., Salehi, P., Sonboli, A., Mohammadi, M. 2006. Essential oil Variation of Salvia officinalis during its Phenological Cycle. Chemistry of Natural Compounds, 42(1), 19-23.
  • Sefidkon, F., Abbasi, Kh., Bakhshi Khaniki Gh. 2006. Influence of Drying and Extraction Method on Yield and Chemical Composition of the Essential Oil of Saturea hortensis. Food Chemistry, 99(1), 19-23.
  • Kizil, S., Toncer, O., Ipek, A., Arslan, N., Saglam, S., Khawar, K. M. 2008. Blooming Stages of Turkish Hyssop (Hyssopus officinalis L.) Affect Essential Oil Composition. Acta Agriculturae Scandinavica, Section B, Soil and Plant Science, 58(3), 273–279.
  • Nurzyńska-Wierdak, R., Dzida, K. 2009. Influence of Plant Density and Term of Harvest on Yield and Chemical Composition of Sweet Marjoram (Origanum majorana L.). Acta Scientiarum Polonorum Hortorum Cultus, 8(1), 51–61.
  • Kaškonienie,V., Kaškonas, P., Maruška, A., Ragažinskiene, O. 2011. Chemical Composition and Chemometric Analysis of Essentials Oils Variation of Bidens tripatita L. during Vegetation Stages. Acta Physiologiae Plantarum, 33, 2377–2385.
  • Georgiou, C., Tzakou, O., Koutsaviti, A., Bazos, I. 2010. Chemical Composition of Echinophora tenuifolia subsp. sibthorpiana Essential Oil from Greece. Records of Natural Products 4(3), 167-170.
  • Özcan, M., Akgül, A., Chalchat, J. C. 2002. Composition of the Essential Oil of Echinophora tenuifolia L. ssp. sibthorpiana (Guss.) Tutin from Turkey. Journal of Essential Oil Research, 14(1), 23-24.
There are 43 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Makaleler
Authors

Arif Şanlı

Tahsin Karadoğan This is me

Bekir Tosun

Muhammet Tonguç This is me

Sabri Erbaş

Publication Date April 15, 2016
Published in Issue Year 2016 Volume: 20 Issue: 1

Cite

APA Şanlı, A., Karadoğan, T., Tosun, B., Tonguç, M., et al. (2016). Growth Stage and Drying Methods Affect Essential Oil Content and Composition of Pickling Herb (Echinophora tenuifolia subsp. sibthorpiana Tutin). Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 20(1), 43-49. https://doi.org/10.19113/sdufbed.59206
AMA Şanlı A, Karadoğan T, Tosun B, Tonguç M, Erbaş S. Growth Stage and Drying Methods Affect Essential Oil Content and Composition of Pickling Herb (Echinophora tenuifolia subsp. sibthorpiana Tutin). SDÜ Fen Bil Enst Der. April 2016;20(1):43-49. doi:10.19113/sdufbed.59206
Chicago Şanlı, Arif, Tahsin Karadoğan, Bekir Tosun, Muhammet Tonguç, and Sabri Erbaş. “Growth Stage and Drying Methods Affect Essential Oil Content and Composition of Pickling Herb (Echinophora Tenuifolia Subsp. Sibthorpiana Tutin)”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 20, no. 1 (April 2016): 43-49. https://doi.org/10.19113/sdufbed.59206.
EndNote Şanlı A, Karadoğan T, Tosun B, Tonguç M, Erbaş S (April 1, 2016) Growth Stage and Drying Methods Affect Essential Oil Content and Composition of Pickling Herb (Echinophora tenuifolia subsp. sibthorpiana Tutin). Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 20 1 43–49.
IEEE A. Şanlı, T. Karadoğan, B. Tosun, M. Tonguç, and S. Erbaş, “Growth Stage and Drying Methods Affect Essential Oil Content and Composition of Pickling Herb (Echinophora tenuifolia subsp. sibthorpiana Tutin)”, SDÜ Fen Bil Enst Der, vol. 20, no. 1, pp. 43–49, 2016, doi: 10.19113/sdufbed.59206.
ISNAD Şanlı, Arif et al. “Growth Stage and Drying Methods Affect Essential Oil Content and Composition of Pickling Herb (Echinophora Tenuifolia Subsp. Sibthorpiana Tutin)”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 20/1 (April 2016), 43-49. https://doi.org/10.19113/sdufbed.59206.
JAMA Şanlı A, Karadoğan T, Tosun B, Tonguç M, Erbaş S. Growth Stage and Drying Methods Affect Essential Oil Content and Composition of Pickling Herb (Echinophora tenuifolia subsp. sibthorpiana Tutin). SDÜ Fen Bil Enst Der. 2016;20:43–49.
MLA Şanlı, Arif et al. “Growth Stage and Drying Methods Affect Essential Oil Content and Composition of Pickling Herb (Echinophora Tenuifolia Subsp. Sibthorpiana Tutin)”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 20, no. 1, 2016, pp. 43-49, doi:10.19113/sdufbed.59206.
Vancouver Şanlı A, Karadoğan T, Tosun B, Tonguç M, Erbaş S. Growth Stage and Drying Methods Affect Essential Oil Content and Composition of Pickling Herb (Echinophora tenuifolia subsp. sibthorpiana Tutin). SDÜ Fen Bil Enst Der. 2016;20(1):43-9.

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