Quorum Sensing Inhibitory Activities of Alpha-Pinene, Terpineol, Cineole, Linalool and Camphor

Year 2016, Volume: 20 Issue: 3, 558 - 565, 25.07.2016

Seyhan Ulusoy , Erdi Doğru

https://doi.org/10.19113/sdufbed.16945

Abstract

Many pathogenic Gram negative bacteria often use N-Acyl homoserine lactone mediated cell to cell communication (quorum sensing) system to control the gene expression of various virulence factors. Thus blocking or manipulating this system makes it an attractive target for discovery of new therapeutics. There are a number of plant-based natural products as quorum sensing inhibitors.. In this study, QS inhibitory potential of laurus, salvia, myrtus and juniperus essential oils were screened and compositions of oils were analysed by GC-MS. The anti-quorum sensing activity of alpha pinene, terpineol, cineole, linalool and camphor (major components of oils) tested against using Chromobacterium violaceum strain CV026, C. violaceum strain VIR07, Pseudomonas aeruginosa PAO1, P. aeruginosa PAC-1. Results showed that alpha pinene, terpineol, cineole, linalool and camphor significantly inhibited violacein production in C. violaceum 026, C. violaceum VIR07, as well as the elastase, pyocyanin production, swarming motilty in P. aeruginosa PAO1, P. aeruginosa PAC-1. Our findings demonstrated that alpha pinene, terpineol, cineole, linalool and camphor have potential as inhibitors of quorum sensing system in C violaceum and P. aeruginosa.

Keywords

Pseudomonas aeruginosa, Quorum sensing, Alpha pinene, Terpineol, Cineole, Linalool

Alfa-Pinen, Terpineol, Sineol, Linelol ve Kamforun Bakteriyel Hücreler Arası İletişim Sistemi Üzerine İnhibitör Etkileri

Abstract

Pek çok Gram negatif patojen bakteri çeşitli virülens faktörlerinin üretiminin genetik seviyede kontrolünü N-Açil homoserin lakton aracılı hücreler arası iletişim sistemini kullanarak sağlar. Bu nedenle bu hücreler arası iletişim sisteminin engellenmesi veya modifiye edilmesi, bu sistemi yeni terapötiklerin keşfi için cazip bir hedef haline getirmektedir. Hücreler arası iletişim sistemi inhibitörü, bitkisel kökenli pek çok doğal ürün bulunmaktadır. Bu çalışmada defne, adaçayı, mersin ve ardıç uçucu yağlarının bakterilerdeki hücreler arası iletişim sistemi üzerine engelleyici özellikleri incelenmiş ve bu yağ örneklerinin majör bileşenleri GC-MS ile belirlenmiştir. Alfa pinen, terpineol, sineol, linelol ve kamforun (yağ örneklerinin majör bileşenleri) hücreler arası iletişim sistemi üzerine inhibitör etkileri C. violaceum, 026, C. violaceum VIR07, Pseudomonas aeruginosa PAO1, P. aeruginosa PAC-1 kullanılarak araştırılmıştır. Sonuçlar alfa pinen, terpineol, sineol, linelol ve kamforun C. violaceum, 026, C. violaceum VIR07 suşunda viyolasin üretiminin hem de, P. aeruginosa PAO1 ve P. aeruginosa PAC-1 klinik izolatında piyosiyanin, elastaz üretimi ve kayma hareketini önemli derecede inhibe ettiğini göstermiştir. Çalışmanın sonuçları alfa pinen, terpieneol, sineol, linelol ve kamforun, C violaceum ve P. aeruginosa için hücreler arası iletişim sistemi üzerine inhibitör etkileri bulunduğu tespit edilmiştir.

Keywords

Pseudomonas aeruginosa, Hücreler arası iletişim, Alfa pinen, Terpineol, Sineol, Linelol

References

• [1] Rasmussen, T. B., Givskov, M. 2006. Quorum sensing inhibitors: a bargain of effects. Microbiology 152, 895-904.
• [2] Fuqua, C., Winans, S.C., Greenberg, E.P. 1996. Census and consensus in bacterial ecosystems: the LuxR-LuxI family of quorum-sensing transcriptional regulators. Annual Reviews in Microbiology 50, 727-751.
• [3] McGowan, S.J., Barnard, A.M., Bosgelmez, G., Sebaihia, M., Simpson, N.J., Thomson, N.R., Todd, D.E., Welch, M., Whitehead, N.A., Salmond, G.P. 2005. Carbapenem antibiotic biosynthesis in Erwinia carotovora is regulated by physiological and genetic factors modulating the quorum sensing‐dependent control pathway. Molecular microbiology 55, 526-545.
• [4] Hentzer, M., Givskov, M., Parsek, M.R. 2002. Targeting quorum sensing for treatment of chronic bacterial biofilm infections. Laboratory Medicine 33, 295-306.
• [5] Parsek, M.R., Greenberg, E.P. 2000. Acyl-homoserine lactone quorum sensing in gram-negative bacteria: a signaling mechanism involved in associations with higher organisms. Proceedings of the National Academy of Sciences 97, 8789-8793.
• [6] Parsons, J.F., Greenhagen, B.T., Shi, K., Calabrese, K., Robinson, H., Ladner, J.E. 2007. Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa. Biochemistry 46, 1821-1828.
• [7] Van Delden, C., Iglewski, B.H. 1998. Cell-to-cell signaling and Pseudomonas aeruginosa infections. Emerging infectious diseases 4, 551.
• [8] Williams, P., Camara, M., Hardman, A., Swift, S., Milton, D., Hope, V.J., Winzer, K., Middleton, B., Pritchard, D.I., Bycroft, B.W. 2000. Quorum sensing and the population-dependent control of virulence. Philosophical Transactions of the Royal Society of London B: Biological Sciences 355, 667-680.
• [9] McClean, K.H., Winson, M.K., Fish, L., Taylor, A., Chhabra, S.R., Camara, M., Daykin, M., Lamb, J.H., Swift, S., Bycroft, B.W. 1997. Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiology 143, 3703-3711.
• [10] Morohoshi, T., Kato, M., Fukamachi, K., Kato, N., Ikeda, T. 2008. N-acylhomoserine lactone regulates violacein production in Chromobacterium violaceum type strain ATCC 12472. FEMS microbiology letters 279, 124-130.
• [11] Bjarnsholt, T., Jensen, P.Ø., Rasmussen, T.B., Christophersen, L., Calum, H., Hentzer, M., Hougen, H.-P., Rygaard, J., Moser, C., Eberl, L. 2005. Garlic blocks quorum sensing and promotes rapid clearing of pulmonary Pseudomonas aeruginosa infections. Microbiology 151, 3873-3880.
• [12] Cavanagh, H., Wilkinson, J. 2002. Biological activities of lavender essential oil. Phytotherapy Research 16, 301-308.
• [13] Hammer, K.A., Carson, C., Riley, T. 1999. Antimicrobial activity of essential oils and other plant extracts. Journal of applied microbiology 86, 985-990.
• [14] Choo, J., Rukayadi, Y., Hwang, J.K. 2006. Inhibition of bacterial quorum sensing by vanilla extract. Letters in Applied Microbiology 42, 637-641.
• [15] Rashid, M.H., Kornberg, A. 2000. Inorganic polyphosphate is needed for swimming, swarming, and twitching motilities of Pseudomonas aeruginosa. Proceedings of the National Academy of Sciences 97, 4885-4890.
• [16] Ohman, D., Cryz, S., Iglewski, B. 1980. Isolation and characterization of Pseudomonas aeruginosa PAO mutant that produces altered elastase. Journal of Bacteriology 142, 836-842.
• [17] Essar, D., Eberly, L., Crawford, I. 1990. Evolutionary differences in chromosomal locations of four early genes of the tryptophan pathway in fluorescent pseudomonads: DNA sequences and characterization of Pseudomonas putida trpE and trpGDC. Journal of bacteriology 172, 867-883.
• [18] Zhang, X.-S., García-Contreras, R., Wood, T.K. 2008. Escherichia coli transcription factor YncC (McbR) regulates colanic acid and biofilm formation by repressing expression of periplasmic protein YbiM (McbA). The ISME journal 2, 615-631.
• [19] Bosgelmez-Tinaz, G., Ulusoy, S., Ugur, A., Ceylan, O. 2007. Inhibition of quorum sensing–regulated behaviors by Scorzonera sandrasica. Current microbiology 55, 114-118.
• [20] Khan, M.S.A., Zahin, M., Hasan, S., Husain, F.M., Ahmad, I. 2009. Inhibition of quorum sensing regulated bacterial functions by plant essential oils with special reference to clove oil. Letters in applied microbiology 49, 354-360.
• [21] Salini, R., Sindhulakshmi, M., Poongothai, T., Pandian, S.K. 2015. Inhibition of quorum sensing mediated biofilm development and virulence in uropathogens by Hyptis suaveolens. Antonie van Leeuwenhoek 107, 1095-1106.
• [22] Szabó, M.Á., Varga, G.Z., Hohmann, J., Schelz, Z., Szegedi, E., Amaral, L., Molnár, J. 2010. Inhibition of quorum‐sensing signals by essential oils. Phytotherapy research 24, 782-786.
• [23] Zhu, H., Sun, S. 2008. Inhibition of bacterial quorum sensing-regulated behaviors by Tremella fuciformis extract. Current microbiology 57, 418-422.
• [24] Zhu, H., Liu, W., Tian, B., Liu, H., Ning, S. 2011. Inhibition of quorum sensing in the opportunistic pathogenic bacterium Chromobacterium violaceum by an extract from fruiting bodies of lingzhi or reishi medicinal mushroom, Ganoderma lucidum (w. Curt.: Fr.) p. Karst.(higher basidiomycetes). International journal of medicinal mushrooms 13.
• [25] Hamood, A.N., Griswold, J., Colmer, J. 1996. Characterization of elastase-deficient clinical isolates of Pseudomonas aeruginosa. Infection and immunity 64, 3154-3160.
• [26] Passador, L., Cook, J.M., Gambello, M.J., Rust, L., Iglewski, B.H. 1993. Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication. Science 260, 1127-1130.
• [27] De Kievit, T.R., Iglewski, B.H. 2000. Bacterial quorum sensing in pathogenic relationships. Infection and immunity 68, 4839-4849.
• [28] Rumbaugh, K.P., Griswold, J.A., Hamood, A.N. 2000. The role of quorum sensing in the in vivo virulence of Pseudomonas aeruginosa. Microbes and infection 2, 1721-1731.
• [29] Anwar, H., Costerton, J. 1990. Enhanced activity of combination of tobramycin and piperacillin for eradication of sessile biofilm cells of Pseudomonas aeruginosa. Antimicrobial agents and chemotherapy 34, 1666-1671.
• [30] Moskowitz, S.M., Foster, J.M., Emerson, J., Burns, J.L. 2004. Clinically feasible biofilm susceptibility assay for isolates of Pseudomonas aeruginosa from patients with cystic fibrosis. Journal of clinical microbiology 42, 1915-1922.