Sıçanlarda D-Galaktoz ile Uyarılmış Yaşlanma Modelinde Kefir’in Beyin Dokusundaki Oksidatif Stres Üzerine Etkisi

Year 2018, Volume: 22 Issue: Special, 221 - 228, 05.10.2018

Emel Serdaroğlu Kaşıkçı , Hatice Kübra Gökalp

Abstract

D-Galaktoz (D-GAL), oksidatif stresi arttırarak doğal yaşlanma sürecini uyaran bir monosakkarittir. Bu çalışmada, yaşlanma ile ilişkili hasarlı modelde oksidatif stres üzerine günlük kefir tüketiminin etkisi araştırıldı. Sıçanlara 8 hafta boyunca D-GAL (300 mg/kg/5 gün), Düşük doz Kefir (DKFR) (0.7 ml/kg/5 gün) ve Yüksek doz Kefir (YKFR) (3.5 ml/kg/5 gün) uygulandı. Bu süre sonunda elde edilen beyin dokusu örneklerinde Glutatyon (GSH) ve Lipid peroksidasyonu (LPO) düzeyleri ile Süperoksit dismutaz (SOD) ve Asetilkolin esteraz (AChE) aktiviteleri ölçüldü. Kontrol grubuna kıyasla D-GAL grubunda GSH düzeyleri ve SOD aktivitelerinde anlamlı bir azalma gözlendi, ancak LPO düzeyleri ve AChE aktiviteleri arttı (p <0.05). Kefirin her iki dozunda da D-GAL verilen gruba kıyasla LPO düzeyleri azaldı (p <0.05). D-GAL grubuna kıyasla, D-GAL verilen düşük doz kefir grubunda SOD aktivitesi arttı, AChE aktivitesi azaldı (p <0.05). Aynı zamanda histopatolojik bulgular da kefirin taklitçi yaşlanma modelinde koruyucu bir etki göstererek oksidatif stresi azalttığını destekledi. Bu sonuçlar kefirin özellikle beyin dokusunda yaşlanma ile uyarılmış oksidatif streste faydalı etkilere sahip olabileceğini gösterdi.

Keywords

D-Galaktoz, Kefir; Yaşlanma; Sıçan

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