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Su Sıcaklığının Doktor Balık (Garra rufa) Türünün Kas, Ağız ve Bağırsak Dokularındaki Katalaz Aktivitesi ve Malondialdehit Düzeyleri Üzerine Etkileri

Year 2019, Volume 23, Issue 1, 191 - 194, 01.04.2019
https://doi.org/10.19113/sdufenbed.483082

Abstract

Bu çalışmada farklı su sıcaklıklarında (18°C ve 34°C) beslenen Garra rufa (doktor balık) türü balıkların bazı dokularında (kas, ağız ve bağırsak) katalaz (CAT) aktiviteleri ve malondialdehit (MDA) düzeyleri incelenmiştir. Deney süresince hiç balık ölümü görülmemiştir. Önemlilik dereceleri farklı olmakla birlikte, 34°C de beslenen doktor balıkların oransal boy artışı, oransal ağırlık artışı ve yem dönüşüm etkinlikleri 18°C de beslenen balıklardan daha düşük bulunmuştur. Bu durum, 34°C su sıcaklığında yaşayan doktor balıkların metabolizmalarının daha yüksek, daha hareketli ve terapiye daha istekli olmalarından kaynaklanmış olabilir. İncelenen dokuların CAT aktiviteleri ve MDA seviyelerinin önemli derecede farklı olduğu tespit edilmiştir. En yüksek CAT aktiviteleri ve MDA seviyelerinin bağırsak dokularında olduğu görülmüştür. Fakat 34°C su sıcaklığında beslenen balıkların incelenen tüm dokularındaki CAT aktiviteleri ve MDA seviyelerinin 18°C su sıcaklıklarında beslenen balıklardan önemli derecede farklı olmadığı tespit edilmemiştir.    

References

  • [1] Ündar, L., Akpınar, M. A., Yanıkoğlu, A. 1990. Doctor fish and psoriasis. The Lancet, 335(1990), 470 - 471.
  • [2] Karahan A. 2007. Comparative investigation of Garra rufa and Garra variabilis by account of sitogenetic and morphometric. Mersin University, Institute of Science, Doctorate Thesis, 123s, Mersin.
  • [3] Sayili, M., Akça, H., Duman, T., Esengün, K. 2007. Psoriasis treatment via doctor fishes as part of healthtourism: A casestudy of Kangal Fish Spring, Turkey. Tourism Management, 28(2007), 625-629.
  • [4] Karaaslan M. 2010. The effects of different stress sources on antioxidant enzyme activities in fishes (Cyprinidae) from Sivas Kangal fish spring. Cumhuriyet University, Institute of Science, Master Thesis, 87s, Sivas.
  • [5] Bhat, M. K. 2000. Cellulases and Related Enzymes in Biotechnology. Biotechnology Advances, 18(2000), 355-383.
  • [6] Mawadza, C., Kaul, R. H., Zvauya, R., Mattiason, B. 2000. Purification and characterization of cellulases produced by two Bacillus strains. Journal of Biotecnology, 83(2000), 177-187.
  • [7] Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1976), 248.
  • [8] Draper, H. H., Hadley, M. 1990. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology 180(1990), 421-431.
  • [9] Aebi, H. 1974. Catalase Methods of Enzymatic analysis, B. HU. New York and London, Academic, Press Inc (1974), 673-677.
  • [10] Akköse, F. 2012. The effects of different temperature on sexual differentation and development in sea bass (Dicentrarchus labrax L., 1758). Çukurova University, Institute of Science, Doctorate Thesis, 110s, Adana.
  • [11] Duman, S. 2010. Cyprinion macrostomus Heckel, 1843 and Garra rufa Heckel, 1843 species of fishes that is living in Kangal (Sivas) Balıklı Çermik Thermal Hot Spring with Topardıç Stream (Sivas) defines some histological parameters and non-specific immune respond. Çukurova University, Institute of Science, Doctorate Thesis, 89s, Adana.
  • [12] Bozkale, Ş. 2010. Isolation of Hsp70 under different stress factors from Sivas Kangal Hot Spring fish species (Cyprinidae). Cumhuriyet University, Institute of Science, Master Thesis, 89s,Sivas.

Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa)

Year 2019, Volume 23, Issue 1, 191 - 194, 01.04.2019
https://doi.org/10.19113/sdufenbed.483082

Abstract

In this study, the catalase (CAT) activities and malondialdehyde (MDA) levels of some tissues (muscle, mouth and intestine) of Garra rufa (doctor fish) fed in different water temperatures (18°C and 34°C) were studied. No fish death was observed during the experiments. Despite the different significance levels, the proportional length increase, the proportional weight increase, food conversion efficiency of doctor fishes fed at 34°C were found to be lower than in fishes fed at 18°C. This can be due to the fact that doctor fishes living in a water temperature of 34°C have a higher metabolism, are more motile, and are more ready to the therapy. The CAT activities and the MDA levels of the tissues studied were found to be significantly different. The highest CAT activities and MDA levels were observed in intestine tissues. But the CAT activities and the MDA levels of the tissues studied for the fishes fed in 34°C water temperature were not significantly different from those of fishes fed in 18°C water temperature.    

References

  • [1] Ündar, L., Akpınar, M. A., Yanıkoğlu, A. 1990. Doctor fish and psoriasis. The Lancet, 335(1990), 470 - 471.
  • [2] Karahan A. 2007. Comparative investigation of Garra rufa and Garra variabilis by account of sitogenetic and morphometric. Mersin University, Institute of Science, Doctorate Thesis, 123s, Mersin.
  • [3] Sayili, M., Akça, H., Duman, T., Esengün, K. 2007. Psoriasis treatment via doctor fishes as part of healthtourism: A casestudy of Kangal Fish Spring, Turkey. Tourism Management, 28(2007), 625-629.
  • [4] Karaaslan M. 2010. The effects of different stress sources on antioxidant enzyme activities in fishes (Cyprinidae) from Sivas Kangal fish spring. Cumhuriyet University, Institute of Science, Master Thesis, 87s, Sivas.
  • [5] Bhat, M. K. 2000. Cellulases and Related Enzymes in Biotechnology. Biotechnology Advances, 18(2000), 355-383.
  • [6] Mawadza, C., Kaul, R. H., Zvauya, R., Mattiason, B. 2000. Purification and characterization of cellulases produced by two Bacillus strains. Journal of Biotecnology, 83(2000), 177-187.
  • [7] Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1976), 248.
  • [8] Draper, H. H., Hadley, M. 1990. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology 180(1990), 421-431.
  • [9] Aebi, H. 1974. Catalase Methods of Enzymatic analysis, B. HU. New York and London, Academic, Press Inc (1974), 673-677.
  • [10] Akköse, F. 2012. The effects of different temperature on sexual differentation and development in sea bass (Dicentrarchus labrax L., 1758). Çukurova University, Institute of Science, Doctorate Thesis, 110s, Adana.
  • [11] Duman, S. 2010. Cyprinion macrostomus Heckel, 1843 and Garra rufa Heckel, 1843 species of fishes that is living in Kangal (Sivas) Balıklı Çermik Thermal Hot Spring with Topardıç Stream (Sivas) defines some histological parameters and non-specific immune respond. Çukurova University, Institute of Science, Doctorate Thesis, 89s, Adana.
  • [12] Bozkale, Ş. 2010. Isolation of Hsp70 under different stress factors from Sivas Kangal Hot Spring fish species (Cyprinidae). Cumhuriyet University, Institute of Science, Master Thesis, 89s,Sivas.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Kazım UYSAL
DUMLUPINAR ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ
0000-0001-6399-6616
Türkiye


Yıldız ÇOLAK This is me
DUMLUPINAR ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ
0000-0001-8753-5873
Türkiye


Mustafa KAVASOĞLU (Primary Author)
DUMLUPINAR ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ
0000-0002-4136-6397
Türkiye

Publication Date April 1, 2019
Published in Issue Year 2019, Volume 23, Issue 1

Cite

Bibtex @research article { sdufenbed483082, journal = {Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi}, eissn = {1308-6529}, address = {}, publisher = {Süleyman Demirel University}, year = {2019}, volume = {23}, number = {1}, pages = {191 - 194}, doi = {10.19113/sdufenbed.483082}, title = {Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa)}, key = {cite}, author = {Uysal, Kazım and Çolak, Yıldız and Kavasoğlu, Mustafa} }
APA Uysal, K. , Çolak, Y. & Kavasoğlu, M. (2019). Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa) . Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi , 23 (1) , 191-194 . DOI: 10.19113/sdufenbed.483082
MLA Uysal, K. , Çolak, Y. , Kavasoğlu, M. "Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa)" . Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 23 (2019 ): 191-194 <https://dergipark.org.tr/en/pub/sdufenbed/issue/39838/483082>
Chicago Uysal, K. , Çolak, Y. , Kavasoğlu, M. "Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa)". Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 23 (2019 ): 191-194
RIS TY - JOUR T1 - Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa) AU - Kazım Uysal , Yıldız Çolak , Mustafa Kavasoğlu Y1 - 2019 PY - 2019 N1 - doi: 10.19113/sdufenbed.483082 DO - 10.19113/sdufenbed.483082 T2 - Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi JF - Journal JO - JOR SP - 191 EP - 194 VL - 23 IS - 1 SN - -1308-6529 M3 - doi: 10.19113/sdufenbed.483082 UR - https://doi.org/10.19113/sdufenbed.483082 Y2 - 2019 ER -
EndNote %0 Süleyman Demirel University Journal of Natural and Applied Sciences Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa) %A Kazım Uysal , Yıldız Çolak , Mustafa Kavasoğlu %T Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa) %D 2019 %J Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi %P -1308-6529 %V 23 %N 1 %R doi: 10.19113/sdufenbed.483082 %U 10.19113/sdufenbed.483082
ISNAD Uysal, Kazım , Çolak, Yıldız , Kavasoğlu, Mustafa . "Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa)". Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 23 / 1 (April 2019): 191-194 . https://doi.org/10.19113/sdufenbed.483082
AMA Uysal K. , Çolak Y. , Kavasoğlu M. Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa). SDÜ Fen Bil Enst Der. 2019; 23(1): 191-194.
Vancouver Uysal K. , Çolak Y. , Kavasoğlu M. Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa). Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2019; 23(1): 191-194.
IEEE K. Uysal , Y. Çolak and M. Kavasoğlu , "Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa)", Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 23, no. 1, pp. 191-194, Apr. 2019, doi:10.19113/sdufenbed.483082

e-ISSN: 1308-6529