Davutoğlan Kuş Cenneti Kilinin Kimyasal ve Mineralojik Yapısının Antimikrobiyal Etkinliği Açısından Değerlendirilmesi

Year 2019, Volume: 23 Issue: Special [en], 147 - 154, 01.03.2019

Şaziye Betül Sopacı

https://doi.org/10.19113/sdufenbed.446772

Abstract

Bu
çalışmada endüstri bölgelerine uzak bir ekolojik ortam olan Davutoğlan Kuş
Cenneti Kıztepe mevki eteklerinden alınan kil örneğinin olası antimikrobiyal
özellikleri incelenmiştir. Bölgeden alınan kil örneklerinin Gram(-) (Escherichia coli, Pseudomonas aeruginosa)
ve Gram(+) (Bacillus subtilis,
Enterococcus faecalis, Staphylococcus aureus) patojen bakteri türleri
üzerindeki antimikrobiyal etkileri araştırılmıştır. Kilin 0.5-1.5 g/mL
konsantrasyon aralığında 10⁵ cfu/mL bakteri inokülasyonlarına karşı
bakteriostatik etkide olduğu gösterilmiştir. Bu sonuçları desteklemek ve
bulguları açıklamak amacıyla antimikrobiyal aktivite çalışmasına paralel olarak
kil örneğinde X-ışınları kırınımı (XRD), X-ışınları floresans (XRF),
Termogravimetri (TG) çalışmaları ile kilin minerolojik ve kimyasal yapısı
belirlenmiştir. Ayrıca kilin gözenek yapısı ve yüzey alanını belirlemek için
taramalı elektron mikroskobu (SEM) ve Brauer-Emmet-Teller (BET) yöntemleri
kullanılmıştır. Tüm bu analizlerden elde edilen sonuçlar antimikrobiyal
aktivite değerleri ile ilişkilendirilmiştir. Sahadan alınan kil örnekleği XRD,
XRF ve TG analiz sonuçlarına göre illit, dolomit ve kalsit içerdiği tespit
edilmiş ve BET sonuçlarına göre kil 57,002 m²/g gibi geniş yüzey
alanına sahip olduğu bulunmuştur. Geniş yüzey alanı kuvvetli absorbans özellik
kazandırdığından ve bu geniş yüzey alanı oksidasyon için uygun bir ara yüzey
olduğundan bakteriyal üremeyi baskıladığı sonucuna varılmıştır.

Keywords

Doğal kil, Antimikrobiyal aktivite, Kil mineralojisi

Evaluation of Chemical and Mineralogical Structure of Davutoğlan Bird Sanctuary Clay for It’s Antimicrobial Efficiency

Abstract

In this
study we examined possible antimicrobial properties of clay specimen taken from
Davutoğlan Bird Sanctuary which is located in Kıztepe skirts as a remote
ecological environment to industrial areas. Antimicrobial effects of Gram (-) (Escherichia coli, Pseudomonas aeruginosa)
and Gram (+) (Bacillus subtilis,
Enterococcus faecalis, Staphylococcus aureus) pathogenic bacteria species
on clay samples collected from the region were investigated. Between 0.5-1.5
g/mL concentration range clay has been shown to affect bacterial growth with
the bacterial inoculations of 10⁵ cfu/mL. X-ray diffraction (XRD),
X-ray fluorescence (XRF) and thermogravimetric (TG) studies of clay minerals
and chemical structure have been determined in parallel with the study of
antimicrobial activity in order to support these results and to explain the
findings. In addition, scanning electron microscopy (SEM) and
Brunauer–Emmett–Teller (BET) methods were used to determine the pore structure
and surface area of the clay. The obtained results  were correlated with antimicrobial activity.
According to XRD, XRF and TG analysis results, illite, dolomite and calcite
were found to be major clay minerals from the samples taken from the area. It
was found that they have a large surface area of 57,002 m²/g
according to BET results. It is also concluded that since the large surface
area gives strong absorbent properties and supplies a suitable interface for
oxidation, suppression of the bacterial growth is observed.

Keywords

Natural clay, Antimicrobial activity, Clay minerology

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