The Evaluation of Salivary and Serum PTX-3 and CRP Levels in Periodontal Diseases of Different Severity

Abstract

Objective: The aim of this study is to evaluate the salivary and serum levels of Pentraxin (PTX)-3 and C-reactive protein (CRP) of individuals who are periodontally healthy or have periodontal diseases with different severity (gingivitis, periodontitis – Stage I and Stage III).
Material-Method: Salivary and serum CRP and PTX-3 levels of 80 non-smoker systemically healthy individuals (periodontally healthy (Group 1), gingivitis (Group 2), Stage I periodontitis (Group 3) and Stage III periodontitis (Group 4) were determined by ELISA, compared among the groups and their correlations were analyzed.
Results: While the highest serum CRP level was determined in Group 4, Group 1 showed significantly lower serum CRP levels from all groups (p<0.05). Group 2 and 3 had similar serum CRP levels (p>0.05), but statistically significantly higher serum CRP levels were found in both groups than Group 1 and lower than Group 4 (p<0.05). Serum PTX-3 was ranked increasing from the healthy group to the groups with severe periodontitis; however, Group 2 presented significantly higher serum PTX-3 level than Group 3 (p<0.05).While salivary CRP and PTX-3 levels were significantly lower in Group 1 than the other groups, and significantly higher in Groups 3 and 4 than the other groups (p<0.05); Group 2 and Group 3 presented similar levels (p>0.05). When the correlations between periodontal parameters and serum and saliva parameters were examined, both CRP and PTX-3 showed strong correlations with all periodontal parameters (p<0.001).
Conclusion: The significant differences between the salivary and serum parameters’ levels among the study groups and the correlations between the parameters provided important data to clarify the periodontal disease-systemic disease relationship.

Keywords

• gingivitis
• periodontitis
• inflammation
• CRP
• PTX3

Farklı Şiddetteki Periodontal Hastalıklarda Salya ve Serumdaki PTX-3 ve CRP Düzeylerinin Değerlendirilmesi

Abstract

Amaç: Bu çalışmanın amacı periodontal açıdan sağlıklı ve farklı şiddetlerde periodontal hastalığı olan (gingivitis, periodontitis –Evre I ve Evre III) bireylerin salya ve serum örneklerinde Pentraksin (PTX)-3 ve C-reaktif protein (CRP) düzeylerinin belirlenmesidir.
Materyal-metot: Sağlıklı (Grup 1, n=20), gingivitis (Grup 2, n=20), Evre I periodontitisli (Grup 3) ve Evre III periodontitisli (Grup 4, n=20) olmak üzere 80 sigara içmeyen sistemik sağlıklı bireyin salya ve serum CRP ve PTX-3 düzeyleri ELISA yöntemiyle belirlenerek gruplar arasında karşılaştırıldı ve klinik parametrelerle korelasyonları incelendi.
Bulgular: En yüksek serum CRP düzeyi Grup 4’te belirlenirken, Grup 1 tüm gruplardan anlamlı düzeyde düşük serum CRP düzeyi sergiledi (p<0,05). Serum CRP düzeylerinin Grup 2 ve Grup 3’te istatistiksel olarak birbirine benzer (p>0,05); ancak Grup 1’den anlamlı yüksek (p<0,05) ve Grup 4’ten anlamlı düşük düzeyde (p<0,05)olduğu belirlendi. Serum PTX-3 sağlıklı gruptan şiddetli periodontitisli gruplarına doğru artan bir düzeyde sıralanmaktaydı; ancak bu sıralamada Grup 2 Grup 3’ten daha yüksek serum PTX-3 düzeyi sergiledi (p<0,05).
Salya CRP ve PTX-3 düzeyleri Grup 1’de diğer gruplardan anlamlı düşük, Grup 3 ve 4’te diğer gruplardan anlamlı yüksek düzeyde iken (p<0,05); Grup 2 ve Grup 3 birbirine benzer düzeyler sergiledi (p>0,05). Periodontal parametreler ve serum ve salya parametreleri arasındaki korelasyonlar incelendiğinde, hem CRP hem de PTX-3’ün tüm periodontal parametreler ile güçlü korelasyonlar sergilediği belirlendi (p<0,001).
Sonuç: İncelenen salya ve serum parametreleri (CRP ve PTX-3) ve çalışma grupları arasında belirlenen düzeysel farklılık ve parametreler arasındaki korelasyonlar periodontal hastalık-sistemik hastalık ilişkisini açıklamak için önemli veriler sunmuştur. 

Keywords

• Gingivitis
• periodontitis
• enflamasyon
• CRP
• PTX-3

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