Relationship of Ghrelin and Ghrelin Receptor Polymorphisms with Type 2 Diabetes

Abstract

There is compelling evidence that the gastric peptide ghrelin and its growth hormone secretagogue G protein-coupled receptor play an important role in glucose metabolism and energy homeostasis. Therefore, these genes carrying susceptibility alleles for Type 2 diabetes are considered as potential candidates. A case-control cross-sectional association study was conducted in Isparta region, including 75 Type 2 diabetes patients and 100 Turkish adult individuals, including 25 controls. Four promoters (rs26311, rs26312, rs27647, rs3755777) and an intronic (rs35683) region in the ghrelin gene and an intronic (rs509035) and a 3'UTR (rs565105) region in the ghrelin receptor are associated with type 2 diabetes by selecting single nucleotide polymorphisms Matrix Assisted Laser Desorption /Ionization was investigated using the Time of Flight Mass Spectrometer system. A significant relationship was found between type 2 diabetes and two single-nucleotide polymorphisms: rs27647 in the promoter region of the ghrelin gene and rs35683 in intron 1 (P<0.05). In conclusion, the findings suggest that ghrelin polymorphisms may predispose to disease development in the Type 2 diabetes population living in the Isparta region.

Keywords

• Type 2 diabetes
• ghrelin
• ghrelin receptor
• single-nucleotide polymorphisms
• Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometer

Grelin ve Grelin Reseptörü Polimorfizmlerinin Tip 2 Diyabetle İlişkisi

Abstract

Bir gastrik peptit olan grelin ve onun büyüme hormonu salgılatıcı G protein-bağlı reseptörünün glukoz metabolizması ve enerji homeostazında önemli bir rol oynadığına dair ciddi kanıtlar mevcuttur. Bu nedenle, Tip 2 diyabet için duyarlılık alelleri taşıyan bu genler potansiyel birer aday olarak değerlendirilmektedir. Isparta yöresinden 75 Tip 2 diyabet hastası ve 25 kontrolden oluşan 100 Türk yetişkin bireyin dahil edildiği vaka-kontrol kesitsel ilişkilendirme çalışması yapıldı. Grelin geninde dört promotör (rs26311, rs26312, rs27647, rs3755777) ve bir intronik (rs35683) bölge ve Grelin reseptöründe bir intronik (rs509035) ve bir 3'UTR (rs565105) bölge tek nükleotid polimorfizmleri seçilerek Tip 2 diyabet ile ilişkisi Matris Destekli Lazer Desorpsiyon/İyonizasyon Uçuş Süresi Kütle Spektrometresi sistemi kullanılarak araştırıldı. Tip 2 diyabet ile iki adet tek nükleotid polimorfizmi arasında anlamlı bir ilişki saptandı: Grelin geninin promotör bölgesinde yer alan rs27647 ve intron 1'de bulunan rs35683 (P<0.05). Sonuç olarak, elde edilen bulgular Isparta yöresinde yaşayan Tip 2 diyabet toplumunda grelin polimorfizmlerinin hastalık gelişimine yatkınlık oluşturabileceğini düşündürmektedir.

Keywords

• Tip 2 diyabet
• grelin
• grelin reseptörü
• tek nükleotid polimorfizmi
• Matris Destekli Lazer Desorpsiyon/İyonizasyon Uçuş Süresi Kütle Spektrometresi

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