Effect of Electromagnetic Radiation (2.45 GHz) on Lymphocyte DNA Damage and Hematological Parameters: The Protective Role of Vitamin C

Abstract

Objective: The aim of this study was to investigate the effects of 2.45 GHz electromagnetic radiation (EMR), which may cause hematological and biochemical changes in blood. We preferred Vitamin C (Vit C) for its protective properties effects against the effects of EMR exposure. Material and Method: For this study, 18 female Sprague Dawley rats were randomly divided into three groups with six animals in each: Sham, EMR and EMR+Vit C groups. Sham group: 0.1ml/day saline for 30 days, by oral gavage; EMR group: EMR, 1h/day for 30 days; EMR+Vit C group: EMR, 1h/day for 30 days+Vit C 250 mg/kg/daily, by oral gavage. White Blood Cell (WBC), Neutrophil, Lymphocyte, Monocyte, Eosinophil, Basophil, Red Blood Cell (RBC), Hemoglobin (Hb), Hematocrit (Htc), Mean Erythrocyte Volume (MCV), Red Cell Distribution Width-SD (RDW-SD), Red Cell Distribution Width-CV (RDW-CV), Thrombocyte (PLT), Mean Platelet Volume (MPV), Platelet Distribution Width (PDW), Platelet Crit (PCT) and Platelet Large Cell Ratio (P-LCR) counts were measured. Lymphocyte DNA damage was assessed by comet assay, additionally, malondialdehyde (MDA) level and catalase (CAT) activity were evaluated. Results: Comet analysis score and P-LCR counts were increased in EMR group compared to Sham group (p<0.05). We observed a decrease in comet analysis score and P-LCR counts after Vit C treatment compared to the finding in EMR group (p<0.05). Conclusions: The results suggest that EMR at the frequency generated by a cell phone causes lymphocyte DNA break and increases P-LCR level. Vit C seems to reduce lymphocyte DNA damage and P-LCR level caused by EMF exposure.

Keywords

• Comet assay
• DNA damage
• electromagnetic radiation
• oxidative stress
• Vitamin C

Elektromanyetik Radyasyonun (2.45 GHz) Lenfosit DNA Hasarına ve Hematolojik Parametrelere Etkisi: Vitamin C'nin Koruyucu Rolü

Abstract

Amaç: Bu çalışmanın amacı, kanda hematolojik ve biyokimyasal değişikliklere neden olabilen 2.45 GHz elektromanyetik radyasyonun (EMR) etkilerini araştırmaktır. EMR maruziyetinin etkilerine karşı koruyucu özelliği olan Vitamin C'yi (Vit C) tercih ettik. Materyal-Metot: Bu çalışma için 18 dişi Sprague Dawley sıçanı rastgele her birinde altı hayvan bulunan üç gruba ayrıldı: Kontrol, EMR ve EMR+Vit C grupları. Kontrol grubu: gavaj ile 30 gün boyunca 0.1 ml/gün salin; EMR grubu: EMR, 30 gün boyunca 1 saat/gün; EMR+Vit C grubu: EMR, 30 gün boyunca 1 saat/gün C vitamini 250 mg/kg/gün, gavaj ile. Beyaz Kan Hücresi (WBC), Nötrofil, Lenfosit, Monosit, Eozinofil, Bazofil, Kırmızı Kan Hücresi (RBC), Hemoglobin (Hb), Hematokrit (Htc), Ortalama Eritrosit Hacmi (MCV), Kırmızı Hücre Dağılım Genişliği-SD (RDW- SD), Kırmızı Hücre Dağılım Genişliği-CV (RDW-CV), Trombosit (PLT), Ortalama Trombosit Hacmi (MPV), Trombosit Dağılım Genişliği (PDW), Trombosit Krit (PCT) ve Trombosit Büyük Hücre Oranı (P-LCR) sayıları ölçülmüştür. Comet testi ile lenfosit DNA hasarı değerlendirildi, ayrıca malondialdehit (MDA) seviyesi ve katalaz (CAT) aktivitesi değerlendirildi. Bulgular: Comet analiz skoru ve P-LCR sayıları EMR grubunda Kontrol grubuna göre arttı (p<0,05). C vitamini tedavisi sonrası comet analiz skorunda ve P-LCR sayılarında EMR grubuna göre azalma gözlemledik (p<0,05). Sonuç: Sonuçlar, bir cep telefonu tarafından üretilen frekansta EMR'nin lenfosit DNA kırılmasına neden olduğunu ve P-LCR seviyesini artırdığını göstermektedir. C vitamini, EMF maruziyetinin neden olduğu lenfosit DNA hasarını ve P-LCR seviyesini azaltıyor gibi görünmektedir.

Keywords

• Comet Analizi
• DNA hasarı
• Elektromanyetik radyasyon
• oksidatif stres
• C vitamini.

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