P. Aeruginosa Bakterisinin Vitamin B Kompleksi ve Kırmızı Lazer Kullanılarak İnaktive Edilmesi

Abstract

Son yıllarda anti-bakteriyel direnç olgusu, bakterilerin neden olduğu hastalıklarla mücadelede daha fazla sorun haline gelmiştir. Biz bu çalışmayla, açık yara ve yanıkların fırsatçı patojeni olan Pseudomonas aeruginosayı daha etkili bir yöntemle inaktive etmeyi ve fotodinamik inaktivasyon(PDI) gelişimine katkıda bulunmayı hedefledik. Pseudomonas aeruginosa bakterisini, vitamin B kompleksi ve lazer ile inaktive olduğunu spektrofotometrik ölçümler ve antimikrobiyal madde etkinliği analiz yöntemleriyle gösterdik. Ayrıca PDI'nin bakteri üremesi üzerindeki etkisi hem kalitatif hem de kantitatif olarak değerlendirilmiştir. Escherichia coli ve Pseudomonas aeruginosa suşları karşılaştırılarak ölüm oranları belirlendi. Koloni oluşturma birimi oranlarında Escherichia coli bakteri suşlarının karanlık ve ışık deneyinde vitamin B kompleksinin 0,25 mg/mL ve 0,125 mg/mL konsantrasyonlarında % 20’lik bir ölüm oranı görülmüştür. Pseudomonas aeruginosa bakteri suşlarının karanlık deneyinde vitamin B kompleksinin 0,5 mg/mL ve 0,125 mg/mL konsantrasyonlarında çok az bir ölüm oranı görülürken lazer maruziyet sonrası ölüm oranları sırasıyla % 60 ve % 50 çıktığı görülmüştür. Bu ölüm oranlarını doğrulama amaçlı akış sitometresi canlılık deneyleri yapılmış ve çıkan sonuçlar birbiriyle paralellik göstermiştir. Bu çalışmadan elde edilen veriler ışığında; kırmızı lazer diyotun, vitamin B kompleksi ile birlikte Pseudomonas aeruginosa bakterisinin inaktivasyonu için uygun bir aday olduğunu öngörmekteyiz. Yaptığımız bu çalışma, bakteriyel enfeksiyonların tedavisi için hastane, tıp ve mikrobiyoloji alanlarında yapılacak alternatif tedavi yöntemlerine ışık tutacaktır.   

Keywords

• Fotodinamik inaktivasyon
• antibiyotik direnç
• hastane enfeksiyonları
• yara patojen bakterileri
• lazer

Inactivation Of P. Aeruginosa Bacteria Using Vitamin B Complex and Red Laser

Abstract

In recent years, the phenomenon of anti-bacterial resistance has become more of a problem in combating diseases caused by bacteria. With this study, we aimed to inactivate Pseudomonas aeruginosa, an opportunistic pathogen of open wounds and burns, with a more effective method and to contribute to the development of photodynamic inactivation (PDI). We have shown that Pseudomonas aeruginosa bacteria are inactivated by vitamin B complex and laser with verifications of both spectrophotometric measurements and antimicrobial agent activity analysis. Moreover, the effect of PDI on bacterial growth was evaluated both qualitatively and quantitatively. Mortality rates were determined by comparing Escherichia coli and Pseudomonas aeruginosa strains. In the dark and light experiment of Escherichia coli bacterial strains at colony forming unit rates, a mortality rate of 20% was observed at 0.25 mg/mL and 0.125 mg/mL concentrations of vitamin B complex. In the dark experiment of Pseudomonas aeruginosa bacterial strains, a very low mortality rate was observed at 0.5 mg/mL and 0.125 mg/mL concentrations of vitamin B complex, while mortality rates after laser exposure were 60% and 50%, respectively. Flow cytometer viability experiments were performed to confirm these mortality rates and the results exhibited consistency with each other. In the light of this study; We predict that the red laser diode, together with the vitamin B complex, is a suitable candidate for the inactivation of Pseudomonas aeruginosa bacteria. This study will shed light on alternative treatment methods for the treatment of bacterial infections in the fields of hospital, medicine, and microbiology.

Keywords

• Photodynamic inactivation
• antibiotic resistance
• hospital infections
• wound pathogenic bacteria
• laser

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