Investigation Of The Antibiofilm Effects Of Cell-free Supernatants Of Probiotic Bacteria Against The Biofilm Formed By Bacteria Obtained From The Ocular Surface

Abstract

Objective: The aim of this study was to investigate the antibiofilm activity of the acellular filtrates of probiotic lactic acid bacteria on the biofilm produced by Listeria monocytogenes, Pseudomonas putida, Pseudomonas stutzeri, Stenotrophomonas maltophilia and Staphyloccocus epidermidis, isolated from the ocular surface.
Material-Method: Lactobacillus rhamnosus 1743, Lactobacillus plantarum1771, Lactobacillus rhamnosus 3111, L. rhamnosus KA1 and L. rhamnosus 1724 were used as lactic acid bacteria. The purity of both lactic acid bacteria and pathogenic test bacteria was checked before use. Cell-free supernatant of lactic acid bacteria were passed through a 22µm filter. For the antibiofilm effect of the acellular filtrate, test pathogenic bacteria cultured in TSB medium were dispersed into the wells. After washing the wells, biofilm formation was evaluated spectrophotometrically at 570 nm according to optical density.
Results: It was observed that acellular filtrates obtained from lactic acid bacteria inhibited biofilm formation at different rates in different test bacteria. The L. rhamnosus KA1 acellular filtrate was the filtrate that reduced the biofilms of all tested bacteria at most. It was also determined that the filtrates of lactic acid bacteria reduced the biofilm of P. stutzeri CZPX 23 at most.
Conclusion: Cell-free supernatants obtained from probiotic lactic acid bacteria can help prevent biofilms formed by bacteria. The effects of acellular filtrates of different lactic acid bacteria on the biofilm formed by different test bacteria may vary. Further studies are needed for the detection and efficacy of a broad-spectrum lactic acid bacteria acellular filtrates.

Keywords

• cell-free supernatant
• lactic acid bacteria
• probiotic
• biofilm
• parabiotic

Göz Yüzeyinden Elde Edilen Bakterilerin Oluşturduğu Biyofilme Karşı Probiyotik Bakterilerin Hücresiz Filtratlarının Antibiyofilm Etkilerinin Araştırılması

Abstract

Amaç: Bu çalışmanın amacı probiyotik laktik asit bakterilerine ait hücresiz filtratların göz yüzeyinden izole edilmiş olan Listeria monocytogenes, Pseudomonas putida, Pseudomonas stutzeri, Stenotrophomonas maltophilia ve Staphyloccocus epidermidis’in ürettikleri biyofilm üzerine antibiyofilm aktivitesinin araştırılmasıdır.
Materyal-Metot: Laktik asit bakterileri olarak Lactobacillus rhamnosus 1743, Lactobacillus plantarum1771, Lactobacillus rhamnosus 3111, L. rhamnosus KA1 ve L. rhamnosus 1724 kullanıldı. Kullanılmadan önce hem laktik asit bakterilerinin hem test bakterilerinin saflıkları kontrol edildi. Laktik asit bakterileri hücresiz filtratları 22µm’lik filtreden geçirildi. Hücresiz filtratın antibiyofilm etkisi için TSB ortamında kültüre edilen test bakterileri kuyucuklara dağıtıldı. Kuyucuklar yıkandıktan sonra 570 nm’de spektrofotometrik olarak optik yoğunluğa göre biyofilm oluşumu değerlendirildi.
Bulgular: Laktik asit bakterilerinden elde edilen hücresiz filtratların farklı test bakterilerinde farklı oranlarda biyofilm oluşumunu inhibe ettiği gözlendi. L. rhamnosus KA1’in hücresiz filtratı test edilen tüm bakterilerin biyofilmlerini ortalama en fazla azaltan filtrattı. Laktik asit bakterilerinin filtratlarının biyofilmi en çok azalttıkları test bakterisi P. stutzeri 23 CZPX olarak tespit edildi.
Sonuç: Probiyotik laktik asit bakterilerinden elde edilen hücresiz filtratlar bakterilerin oluşturduğu biyofilmlerin önlenmesinde yardımcı olabilir. Farklı LAB hücresiz filtratlarının farklı test bakterilerinin oluşturduğu biyofilme etkileri değişebilmektedir. Geniş spekturumlu bir LAB hücresiz filtratın tespiti ve etkinliği için ileri çalışmalara ihtiyaç vardır.

Keywords

• Hücresiz filtrat
• laktik asit bakterisi
• probiyotik
• biyofilm
• parabiyotik
• cell-free supernatant
• lactic acid bacteria
• probiotic
• biofilm
• parabiotic

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