Echinacea purpurea Ekstraktı Kullanılarak Selenyum-Gümüş Bimetalik Nanopartiküllerin Fitofabrikasyonu: Karakterizasyon ve Antioksidan Aktivite

Abstract

Bu çalışmada Echinacea purpurea sulu ekstraktı kullanılarak selenyum-gümüş bimetalik nanopartiküllerin (Se-Ag BNP'ler) fitofabrikasyonu araştırılmaktadır. Sentez süreci, başarılı nanoparçacık oluşumunu gösteren bir işaret olan koyu kırmızımsı kahverengiye gözle görülür bir renk değişimi ile karakterize edildi. UV-görünür spektrum analizi, sırasıyla 268 nm ve 325 nm'de maksimum absorpsiyonla SeNP'lerin ve AgNP'lerin varlığını doğruladı. X-ışını kırınımı (XRD) desenleri, sentezlenen Se-Ag BNP'lerin kristal yapısını ortaya çıkardı ve metalik gümüş-selenyum nanopartikülleri ile tutarlı karakteristik tepe noktaları sergiledi. Transmisyon elektron mikroskobu (TEM) analizi, Se-Ag BNP'lerin ağırlıklı olarak küresel olan ancak aynı zamanda altıgen ve oval şekillere sahip olan çeşitli morfolojik yapılarını sergilemektedir. Ortalama parçacık boyutunun 33.38 nm olduğu belirlendi, bu da tekdüzelik ve stabiliteyi göstermektedir. Ayrıca Se-Ag BNP'lerin antioksidan özellikleri, sırasıyla 264.78 µg/mL ve 344.19 µg/mL IC50 değerleriyle doza bağlı temizleme yeteneklerini gösteren DPPH ve ABTS radikal temizleme analizleri yoluyla değerlendirildi. Bu bulgular, biyotıp ve çevresel iyileştirme gibi çeşitli alanlarda umut verici uygulamalar sunan Se-Ag BNP'lerin etkili antioksidanlar olarak potansiyelini vurgulamaktadır. Önceki çalışmalarla yapılan karşılaştırmalar, üstün antioksidan aktiviteye sahip Se-Ag BNP'lerin üretiminde Echinacea purpurea ekstraktının kullanıldığı biyosentez yönteminin etkinliğini vurgulamaktadır.

Keywords

• Selenyum
• Gümüş
• Bimetalik
• Nanopartiküller
• Ekinezya
• Antioksidan

Phytofabrication of Selenium-Silver Bimetallic Nanoparticles Using Echinacea purpurea Extract: Characterization and Antioxidant Activity

Abstract

The phytofabrication of selenium-silver bimetallic nanoparticles (Se-Ag BNPs) utilizing Echinacea purpurea aqueous extract is investigated in this study. The synthesis process is characterized by a visible color change to dark reddish brown, a sign indicating successful nanoparticle formation. UV-visible spectrum analysis confirms the presence of SeNPs and AgNPs with absorption maxima at 268 nm and 325 nm, respectively. X-ray diffraction (XRD) patterns reveal the crystalline structure of the synthesized Se-Ag BNPs, exhibiting characteristic peaks consistent with metallic silver and selenium nanoparticles. Transmission electron microscopy (TEM) analysis showcases the diverse morphological structures of the Se-Ag BNPs, predominantly spherical but also featuring hexagonal and oval shapes. The average particle size is determined to be 33.38 nm, indicating uniformity and stability. Furthermore, the antioxidant properties of Se-Ag BNPs are evaluated through DPPH and ABTS radical scavenging assays, demonstrating dose-dependent scavenging capabilities with IC50 values of 264.78 µg/mL and 344.19 µg/mL, respectively. These findings underscore the potential of Se-Ag BNPs as effective antioxidants, offering promising applications in various fields such as biomedicine and environmental remediation. Comparisons with previous studies highlight the efficacy of the biosynthesis method using Echinacea purpurea extract in producing Se-Ag BNPs with superior antioxidant activity.

Keywords

• Selenium
• Silver
• Bimetallic
• Nanoparticles
• Echinacea purpurea
• Antioxidant

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