EFFECT OF MELATONIN ON SPINAL CORD INJURY INDUCED LIPID PEROXIDATION IN RATS: PROGNOSTIC VALUE OF MALONDIALDEHYDE

Abstract

Melatonin is a potent antioxidant agent produced by the pineal gland. In this study we aimed to investigate the neuro-protective effect of melatonin on ischemic spinal cord in the rats. Aortic clamp was used for the occlusion of the abdominal aorta for 30 minutes. Twenty four Winstar male rats were used for this study and rats were divided randomly into 4 groups: control group (sham group, n: 5), sham+melatonin treated group (receiving 10mg/kg melatonin intraperitoneally, n: 5), ischemia/reperfusion group (undergoing occlusion but receiving no pharmacologic intervention, n: 7), melatonin + ischemia/reperfusion group (undergoing occlusion and receiving 10mg/kg melatonin intraperitoneally 10 minutes after ischemia, n: 7). Spinal cord samples were taken for the malondialdehyde (MDA) analyses. In the aort occlusion group, the MDA levels, indicating the extend of lipid peroxidation, were significantly higher than the other groups (p<0.05). Also in the aort occlusion + melatonin group the levels of MDA was significantly reduced compared to the aort occlusion group (p<0.05). In this study, we showed that injection of melatonin reduces the effects of secondary injury and facilitate the recovery of the damaged spinal cord.

Keywords

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RATLARDA LİPİD PEROKSİDASYONUNUN İNDÜKLEDİĞİ SPİNAL KORD HASARINDA MELATONİNİN ETKİSİ: MALONDİALDEHİTİN TANISAL ÖNEMİ

Abstract

Melatonin, pineal bez tarafından üretilen güçlü bir antioksidan ajandır. Bu çalışmada biz, ratlarda spinal kord iskemisinde melatoninin nöroprotektif etkisini araştırmayı amaçladık. Abdominal aortun oklüzyonu için 30dk süre ile aortik klemp kullanıldı. Çalışmada 24 Winstar erkek rat kullanıldı ve ratlar randomize olarak 4 gruba ayrıldı: kontrol grubu (sham grup, n:5), sham + melatonin verilen grup (10mg/kg melatonin intraperitoneal olarak verildi, n:5), iskemi/reperfüzyon grubu (oklüzyon yapılan ancak herhangi bir farmakolojik ajan verilmeyen grup, n:5), melatonin + iskemi/reperfüzyon grubu (oklüzyon yapılan ve iskemiden 10 dk sonra intraperitoneal olarak 10mg/kg melatonin verilen grup). Spinal kord örnekleri malondialdehit (MDA) analizi için alındı. Aort oklüzyonu yapılan grupta, lipid peroksidasyonunun olduğunu gösteren MDA düzeyleri diğer gruplardan belirgin olarak daha yüksekti (p<0.05). Aort oklüzyonu+melatonin verilen grupta da aort oklüzyonu yapılan grup ile karşılaştırıldığında MDA düzeylerinin belirgin olarak azaldığı görüldü (p<0.05). Bu çalışmada biz, melatonin enjeksiyonunun sekonder hasarın etkilerini azalttığını ve hasarlı spinal kordun iyileşmesini kolaylaştırdığını gösterdik.

Keywords

• melatonin, iskemi/reperfüzyon, spinal kord, oksidatif stress, malondialdehit

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